FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212478
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT173178.150020237389283No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG151497.129679307975414No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT142216.692928209038112No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT117815.544574026487449No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG98714.64565743276951No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA73233.4464744585321774No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG71353.357994710040569No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC68493.223392539462909No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG67213.1631510085750056No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC56322.6506273590677623No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA45402.1366917986803338No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA40971.9281996253729796No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT35951.6919398714219824No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTGGCTCTGAGG32011.506508909157654No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG30031.413322791065428No Hit
TCCTCAGCAGTCTGGAACCCAGGAAGTGGTGATGAGGGAGAAGGGGGTAC26561.2500117659240015No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT25951.221302911360235No Hit
AGAGACATCTTAAAGAGGGAAGCTGATGAGAGAGGAAGAGAGTGACAACA23101.087171377742637No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG18870.8880919436365176No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT18700.880091115315468No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA17480.8226734061879348No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC16590.7807867167424393No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTGGTGTCCTTTGCATTCAGTATG15560.7323111098560793No Hit
TCCCTGCTTCTGTCTCCTACCGTAGGAAAAAGAAAATCAGCCTGCACTGT15200.7153681792938563No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA15120.7116030836133623No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG14850.6988958856916951No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA14580.686188687770028No Hit
GGAGAGCTGTAAATTCTGGCTTTTTTGTGTGCCTCATGGAGGGTATGTAG13920.6551266484059526No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT13720.6457139092047177No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG13120.6174756916010128No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA13060.6146518698406424No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG12950.6094748632799631No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC12230.5755890021555173No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT10120.4762846035824885No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT10120.4762846035824885No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC8840.41604307269458485No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA8800.4141605248543378No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA8620.40568905957322643No Hit
GAAAGCCAGGGAGTTGGGGCAGTAGGGGGGCTTTCTCTGTAGAACCATGT8530.40145332693267066No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT8150.38356912245032426No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT7650.3600372744472369No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT7620.35862536356705166No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT6920.32568077636272935No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA6780.3190918589218648No Hit
AGGAGCCTTTCATCCGAAGGCAATCTGAGTTATCTGTTGCTGTAGAACCA6390.3007370174794567No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT6300.296501284838901No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5850.2753226216361223No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT5580.2626154237144551No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG5500.25885032803396113No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5240.24661376707235572No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG5220.24567249315223225No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4910.23108274739031806No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG4850.22825892562994757No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA4750.22355255602933008No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG4650.21884618642871262No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG4600.2164930016284039No Hit
CTTGGCCATAAGAAGCTCCCTTAAATGTCAGCTTCTGATGTAGAACCATG4500.21178663202778641No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC4430.20849217330735414No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT4290.2019032558664897No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTACCCACCTTAGTTTTAACC4140.19484370146556348No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4060.19107860578506952No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG3950.18590159922439028No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA3890.18307777746401982No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA3870.18213650354389632No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC3810.17931268178352583No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA3650.17178249042253785No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA3630.17084121650241438No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC3240.1524863750600062No Hit
CGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG3070.1444855467389565No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA3040.14307363585877125No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2970.13977917713833904No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA2960.13930854017827726No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2930.13789662929809204No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG2900.1364847184179068No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG2790.13130771185722756No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA2580.12142433569593088No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG2570.12095369873586913No Hit
CTGGGATAAAAGTTGCAGCAAGGCCGTGTCTCGGGTAATTCTCTCCGAAG2540.11954178785568388No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT2450.11530605521512816No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA2380.11201159649469593No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG2350.11059968561451068No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2270.1068345899340167No Hit
AATTCTGCTGAATGGGTTGCATGTGTGGAAGCTCTAAGGGATGTAGAACC2220.10448140513370796No Hit
CCCTGTCCCTCTCTCTTCCTCCTCCTCCTCCTCCTGTGTGTTTAGGAGGA2190.10306949425352271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)