FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61284
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC680811.108935448077801No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC679211.082827491678088No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG639110.428496834410286No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC25154.1038443965798574No Hit
TTTCGCTTCCCACAGGTCTCCTGATGGCAAGCTGAGGAGACCAAGTCTCT18563.028522942366686No Hit
TATCAACTGACCCAGGCAAAAGGAAGAGTGGCGGGGAAGAAAAGTGCCAG18192.96814829319235No Hit
TACTGAATGCAAAGGACACCACAGAAAGCTGCCCTTCCCTGTCCTTGCCA17542.862084720318517No Hit
GCTCCAAACAAGATGATAGCCCCGATGACACATTCCTGCTCTTAGTGTCT16822.7445989165198097No Hit
TGGTATGCGACTGTATTGATGGAAGGTAGCACAGAGATTCCAGACCAAGT16462.6858560146204558No Hit
CTTTCTAGTTGATACGGCCTTCACAGGGCTGTTATCTCAGTGGAGTTAAG15612.547157496246981No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC15342.5031003198224657No Hit
GATTCCCGCGCCCAGAGAGATTTGGGGAGGATCTTGGAGGCAGTCCCACG12161.984204686378174No Hit
CTGGTGTTGTTGGGCAGTGTGGAGACAAAAACAGCCCCAGAAAGACCAAG10851.770445793355525No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA8651.411461392859474No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA8601.4033026564845636No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA7151.1666993016121663No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGT6481.057372234188369No Hit
TGGAGCCACATAACACATTCAAAGAAAGATGATGGGAAGTGTGTGTGTGT6241.0182102995887998No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT5890.9610991449644279No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTCCAATCAAGCAGTAGCTGTAACT5820.9496769140395536No Hit
TGTGGTCTGCCAGCTAAAGGCCAGATGGGAGCCCACTTCATTAGTACTGG5460.8909340121401997No Hit
CCTTCTTCCGATAGGTTTTCCCAGTGACACCTTTATCACTCACAAGACCA5220.8517720775406304No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA4300.7016513282422818No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATG4060.6624893936427126No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATGTCAG3870.6314861954180536No Hit
ATATATGTCTTCCACCCTTGACTCTCTTGGCTAAGGTGGGGTGTGGGGGT3860.6298544481430716No Hit
TGCAATTCAGTACAATTAGGTGGGACACTGACGACATGGTTCAGCTTTCG3790.6184322172181973No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC3610.5890607662685203No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3500.5711115462437178No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATGTCAGC3410.5564258207688794No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3360.5482670843939691No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC3170.5172638861693101No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA3160.515632138894328No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT3080.5025781606944717No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA3080.5025781606944717No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATGTC3070.5009464134194895No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3070.5009464134194895No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTCAGTGTAGTACTGAGCACAAC2880.4699432151948306No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT2690.4389400169701717No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT2590.42262254422035117No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT2570.41935904967038706No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATGT2320.37856536779583577No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2180.3557209059460871No Hit
CTTCACCTGCTATTGTTGCAAGAATAGCAGGATGATATTCTTGGGGGTGG2110.3442986750212127No Hit
AAATGTCCCAAAGCTAAATCCACCACTGACTCTGATCCAGAAGACCAAGT2090.3410351804712486No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATGTC1710.27902878402193065No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT1660.2708700476470204No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1570.25618432217218196No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATGTCA1540.25128908034723585No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1240.20233666209777432No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATGTCA1160.18928268389791789No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1160.18928268389791789No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC1020.1664382220481692No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATGTCAG1020.1664382220481692No Hit
CTGGTGTTGTTGGGCAGTGTGGAGACAAAAACAGCCCCAGAGAGACCAAG910.14848900202336662No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA860.14033026564845638No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT790.128908034723582No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATGTCAGCT770.12564454017361792No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATGTCAGCTTAATC760.12401279289863587No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATG750.1223810456236538No Hit
CTGGTGCTGGAACTCTGGGGCCCTCTGGCTGTTCAATCCAACCCTCCTAA750.1223810456236538No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT740.12074929834867175No Hit
GCCCTGCTCCTATAGCCAATACTGACGACATGGTTCTACAGAGTCCCACG720.11748580379870766No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA670.1093270674237974No Hit
GCCCTGCTCCTATAGCCAACACTGACGACATGGTTCTACAGAGTCCCACG660.10769532014881535No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA640.10443182559885125No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA620.10116833104888716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)