FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61284
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG678311.06814176620325No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG621910.147836303113374No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT619110.102147379413877No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA25044.085895176555055No Hit
CCTCAGCTTGCCATCAGGAGACCTGTGGGAAGCGAAATGTAGAACCATGT18633.0399451732915606No Hit
GGCACTTTTCTTCCCCGCCACTCTTCCTTTTGCCTGGGTCAGTTGATATG18212.9714117877423147No Hit
GGCAAGGACAGGGAAGGGCAGCTTTCTGTGGTGTCCTTTGCATTCAGTAT17342.829449774818876No Hit
CCAGACACTAAGAGCAGGAATGTGTCATCGGGGCTATCATCTTGTTTGGA16582.7054369819202404No Hit
GGAATCTCTGTGCTACCTTCCATCAATACAGTCGCATACCATGTAGAACC16502.6923830037203835No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA15182.4769923634227533No Hit
TGGGTTGCTTAACTCCACTGAGATAACAGCCCTGTGAAGGCCGTATCAAC14182.313817635924548No Hit
GAGGGGGTCGTGGGACTGCCTCCAAGATCCTCCCCAAATCTCTCTGGGCG12101.9744142027282814No Hit
TTCTGGGGCTGTTTTTGTCTCCACACTGCCCAACAACACCAGTGTAGAAC10611.7312838587559558No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT8561.3967756673846354No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT8381.3674042164349585No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT7021.1454865870373996No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA6491.059003981463351No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC5800.9464134194895896No Hit
AGTTACAGCTACTGCTTGATTGGACACTGACTCCAAAGAGGAATCTAAGC5760.9398864303896612No Hit
CCAGTACTAATGAAGTGGGCTCCCATCTGGCCTTTAGCTGGCAGACCACA5420.8844070230402715No Hit
TGTGAGTGATAAAGGTGTCACTGGGAAAACCTATCGGAAGAAGGTGTAGA5160.8419815938907381No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT4300.7016513282422818No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4070.6641211409176947No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3930.6412766790679459No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3880.6331179426930357No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG3620.5906925135435024No Hit
GCTTTCAAAACGAAAGCTGAACCATGTCGTCAGTGTCCCACCTAATTGTA3600.5874290189935383No Hit
TTGACATACCTCAGCACACACACACACACACACTTCCCATCATCTTTCTT3500.5711115462437178No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG3440.5613210625938255No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3400.5547940734938973No Hit
ACAAGTCAACAACCCCCACACCCCACCTTAGCCAAGAGAGTCAAGGGTGG3380.5515305789439331No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3300.5384766007440768No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA3190.5205273807192742No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT3110.5074734025194179No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG3090.5042099079694538No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT3020.4927876770445793No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT3010.4911559297695973No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3010.4911559297695973No Hit
CATTCCTTGTTGTGCTCAGTACTACACTGACGACCATAAATAACTTACTT2810.45852098426995624No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2640.4307812805952614No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT2470.4030415769205665No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2340.3818288623457999No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA2320.37856536779583577No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2120.3459304222961948No Hit
CACTCCACCCCCAAGAATATCATCCTGCTATTCTTGCAACAATAGCAGGT2120.3459304222961948No Hit
TCTGGATCAGAGTCAGTGGTGGATTTAGCTTTGGGACATTTTGTAGAACC2050.33450819137132043No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1750.2855557731218589No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT1660.2708700476470204No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1540.25128908034723585No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1530.24965733307225377No Hit
CGGGTTGCTTAACTCCACTGAGATAACAGCCCTGTGAAGGCCGTATCAAC1280.2088636511977025No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1260.2056001566477384No Hit
TTGACATACCTCAGCACACACACACACACACTTCCCATCATCTTTCTTTG1210.19744142027282816No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1160.18928268389791789No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1110.18112394752300762No Hit
GAGGGGGTCGTGGGACTCTGTAGAACCATGTCGTCAGTATTGGCTATAGG1040.16970171659813327No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1030.1680699693231512No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA1010.16480647477318713No Hit
CTCTGGGGCTGTTTTTGTCTCCACACTGCCCAACAACACCAGTGTAGAAC900.14685725474838457No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT790.128908034723582No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG770.12564454017361792No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT770.12564454017361792No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC740.12074929834867175No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG730.11911755107368971No Hit
GAGGGGGTCGTGGGACTCTGTAGAACCATGTCGTCAGTGTTGGCTATAGG720.11748580379870766No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC700.11422230924874356No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT620.10116833104888716No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT620.10116833104888716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)