FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70133
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG944413.465843468837779No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC849512.11270015541899No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC753410.74244649451756No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC64219.155461765502688No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT30984.417321375101593No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT19852.830336646086721No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT15362.1901244777779363No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA13671.9491537507307544No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA12171.7352744071977528No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTA11331.6155019748192718No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT10801.5399312734376114No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT10521.500007129311451No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA9701.3830864215134102No Hit
ACACAGATTCTGAAATGAGAGCTACCGTATGGATGATGATGTTGAAATAT7641.089358789728088No Hit
TTGTGGCACCAAATACGAAACACTGGCATGTTACAGCGGGAGAGACAACC7131.0166398129268677No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTACAC6110.8712018593244265No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTACACG6090.8683501347439865No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA6000.8555173741320063No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA6000.8555173741320063No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC5930.8455363381004662No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5310.7571328761068256No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA5170.7371708040437455No Hit
TGCTAATTGGGCAGATAGAGCTTCTGGATTGAAAACTCCTCAGCCATTTT5160.7357449417535254No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5030.7172087319806653No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTAC4920.7015242467882452No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT4490.6402121683087848No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA4380.6245276831163646No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT4320.6159725093750446No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTA4020.5731966406684442No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3670.5232914605107439No Hit
TGACAGTTTGACAGTTAAAGGCATACACTGACGACATGTTTCTTCTATAA3450.4919224901259036No Hit
GCCCTCTCAGAAACATCTGCTACCGTAACTTCGAGGGCTAAACCACAAGA3020.43061041164644315No Hit
TCAGAAGCTGACATTTAAGGGAGGAGAAGCAGAGAGGGAGACCAAGTCTC2800.39924144126160294No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2760.3935379921007229No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTAC2570.3664466085865427No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT2470.3521879856843426No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA2390.3407810873625825No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTACA2340.3336517759114825No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT2260.32224487758972237No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG2100.29943108094620224No Hit
GGTAGGATGTGATATTCCTTCTAGTGGTACGGTAGCAGAAAGTCAGTCCC2040.29087590720488216No Hit
CATCTCCATGAGTAGGAAGAAGGAGGAGAAGCAGAGAGGGAGACCAAGTC1820.2595069368200419No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA1700.24239658933740182No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA1650.23526727788630175No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1570.2238603795645417No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTACAC1540.21958279269388162No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTACA1490.21245348124278154No Hit
CTGAAAGCTGTACCATACCTGTCTGGTTTTGGCTGAGGTTTCAATGAATG1460.20817589437212153No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC1430.2038983075014615No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTACACGTTCATC1200.1711034748264013No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT1130.16112243879486118No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA1100.15684485192420117No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATT1020.14543795360244108No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA990.14116036673178103No Hit
ACACTTGGCCCTGTCACTTTTTTACGGTAGCAGAGCTGCAGAAACACAAT970.13830864215134103No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTATTA970.13830864215134103No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA940.134031055280681No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATTACACGT880.12547588153936093No Hit
CAGCATCCTGCCCTTACCTGGTTTTCACAGACAGGGGCCAGGAGGAGACC880.12547588153936093No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA860.1226241569589209No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGGAGACCAAGTCT820.11692070779804085No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG820.11692070779804085No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTATT790.11264312092738084No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTATTACA780.11121725863716082No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC760.10836553405672081No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTATTACAC730.10408794718606076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)