FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70133
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT930313.264796885916757No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG796811.361270728473045No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG750710.703948212681619No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA63959.118389345956967No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC30894.404488614489613No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT17142.4439279654370982No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC15102.153052058232216No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT13621.9420244392796544No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT12411.769495102163033No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA11311.612650250238832No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT10321.471489883507051No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA10291.4672122966363907No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG9591.36740193632099No Hit
GGAACATATTTCAACATCATCATCCATACGGTAGCTCTCATTTCAGAATC7811.1135984486618282No Hit
TTTTTCAGTGGTTTAGGAGGGTTGTCTCTCCCGCTGTAACATGCCAGTGT6230.8883122068070666No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC6100.8697759970342065No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT6100.8697759970342065No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT5960.8498139249711262No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA5930.8455363381004662No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG5850.8341294397787061No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5300.7557070138166055No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT5110.7286156303024254No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG4970.7086535582393453No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4850.6915432107567051No Hit
ACTGACTACACAAAAATGGCTGAGGAGTTTTCAATCCAGAAGCTCTATCT4670.665877689532745No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4440.6330828568576847No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4310.6145466470848245No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA4290.6116949225043845No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT4250.6059914733435044No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA3870.5518087063151441No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3670.5232914605107439No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTATGCCTTTAACT3300.4705345557726035No Hit
TGTGGTTTAGCCCTCGAAGTTACGGTAGCAGATGTTTCTGAGAGGGCTGT3030.43203627393666316No Hit
CCCTCTCTGCTTCTCCTCCCTTAAATGTCAGCTTCTGATGTAGAACCATG2850.406370752712703No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2740.3906862675202829No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT2510.3578914348452227No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2510.3578914348452227No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG2400.3422069496528026No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2370.3379293627821425No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT2230.3179672907190624No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT2070.2951534940755422No Hit
CCATGGGACTGACTTTCTGCTACCGTACCACTAGAAGGAATATCACATCC2020.2880241826244421No Hit
CCCTCTCTGCTTCTCCTCCTTCTTCCTACTCATGGAGATGTGTAGAACCA1790.2552293499493819No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT1630.23241555330586172No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC1570.2238603795645417No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1570.2238603795645417No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1540.21958279269388162No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1490.21245348124278154No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1450.20675003208190154No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTTCATT1250.1782327862775013No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1190.16967761253618127No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC1130.16112243879486118No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT1090.15541898963398115No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG1030.14686381589266106No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG980.13973450444156105No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT970.13830864215134103No Hit
GTCAAATTGTGTTTCTGCAGCTCTGCTACCGTAAAAAAGTGACAGGGCCA950.135456917570901No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA950.135456917570901No Hit
CCTCCTGGCCCCTGTCTGTGAAAACCAGGTAAGGGCAGGATGCTGTGTAG880.12547588153936093No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG870.12405001924914091No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT850.12119829466870091No Hit
CCTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT820.11692070779804085No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG790.11264312092738084No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC790.11264312092738084No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG760.10836553405672081No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG730.10408794718606076No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT720.10266208489584078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)