FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209405
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG140296.699457988109167No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC136466.516558821422603No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA112935.392898927914806No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT100104.780210596690623No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC88724.236766075308612No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC82003.9158568324538576No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA63463.0304911535063632No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCACAGCATAAATAACTTACAG61992.9602922566318854No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT61552.9392803419211577No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT55662.658007210907094No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT48912.33566533750388No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA46942.241589264821757No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA41471.9803729614861154No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA39371.8800888230940043No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT38331.830424297414102No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA34201.6331988252429503No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA31651.5114252286239584No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA31071.4837277046870896No Hit
CTGTGCCTCTGTGTGCTGACTAGCAGAGACAGGTATTGCACATGGTATAT29041.386786370908049No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA26641.2721759270313506No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG25981.2406580549652588No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA21931.0472529309233303No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA21211.012869797760321No Hit
GGATACAGTAGCCAGAATGACGACATGGTTTCTACAGGTGCCAGTTTGCT20350.9718010553711708No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC19220.9178386380458919No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG17530.8371337838160502No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA16640.7946324108784413No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT16480.7869917146199947No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC14690.701511425228624No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC14100.6733363577756023No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC12440.5940641340942193No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG12300.5873785248680786No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC12100.5778276545450204No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGAACTAT11750.5611136314796686No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTACGGGTAGCAAATACTGAGCATC11560.5520403046727633No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGAACTA10590.505718583605931No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT10300.49186982163749676No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10260.489959647572885No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT9930.47420071153983906No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAA9760.4660824717652396No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC9360.4469807311191233No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA8680.41450777202072536No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC8560.40877724982689045No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC8490.40543444521382005No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA8450.40352427114920847No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGAAC8090.38633270456770374No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC7610.3634106157923641No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG7600.36293307227621113No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC6900.3295050261455075No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA6520.31135837253169696No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAA6260.2989422411117213No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG5490.2621713903679473No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG5460.2607387598194886No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG5380.2569184116902653No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG5320.2540531505933478No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT4840.2311310618180082No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT4670.2230128220434087No Hit
CTCTCTAGGGGGTCTGTCTTCTCATTTGTGCTAAAACTTTAAGGTGAGAG4600.21967001743033837No Hit
AGCAAGCTGTGACGGGTAAGATAGACCAAGTCTTTGAGACACGCCTTACA4500.21489458226880923No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG4460.21298440820419762No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT4140.19770301568730453No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG4020.1919724934934696No Hit
TGGTGATTTGCTGATATACCATGTCTCTGCTACCGTATTATCCTATTCCT3910.18671951481578758No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG3870.18480934075117594No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT3870.18480934075117594No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGAACT3780.18051144910579978No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT3550.16952794823428285No Hit
TCTTGGTTGTCAGTGCTTCAGTATACGGTACCAGTCAAATGTCCATGGAT3440.16427496955660084No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3420.16331988252429502No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT3410.1628423390081421No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT3330.15902199087891883No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGAACTA3150.1504262075881665No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGAAC3130.14947112055586065No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT3080.14708340297509612No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC3070.1466058594589432No Hit
GTTGTATCCGCTGCTTTGTCCCGCGTAGGCAACCACTGACACAATGGCAA3070.1466058594589432No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT3000.14326305484587284No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC2960.1413528807812612No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA2880.13753253265203794No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG2870.13705498913588499No Hit
GTGCACATTCAAAAAAGAAATTATCCACACTGACGACATGGTTCTTCAGT2730.1303693799097443No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG2570.12272868365129773No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGAACTATCACATC2500.11938587903822737No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA2440.1165206179413099No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGAACT2420.1155655309090041No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC2340.11174518277978082No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT2160.10314939948902843No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC2130.1017167689405697No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT2120.1012392254244168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)