FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175699
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC153418.731409968184224No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG150278.552695234463485No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA128847.332995634579594No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT124337.076306638057132No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT80574.585683470025441No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC80484.580561073199051No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA67383.8349677573577536No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA65043.701785439871599No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA60133.4223302352318457No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC44922.556645171571836No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT44762.547538688324919No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA32731.8628449791973773No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT27851.585097240166421No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA23181.3193017603970427No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT21721.2362051007689288No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC20551.169613942025851No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT17550.9988673811461648No Hit
GTTAGAAGGCTGGCTCCCATGGATCACACATAGGAAGGACAGTGTGAAAA17180.9778086386376702No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG17040.9698404657966181No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA15560.8856054957626395No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG15100.8594243564277543No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA14770.8406422347309888No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC13990.7962481289022704No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAT13520.7694978343644528No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG12330.7017683652155107No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC10380.5907831006437145No Hit
GCCGGAACAGCCCCAGCCTTCCTTAGAAGACAGAAACATGCCCCCGAAGC9000.5122396826390588No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA8680.4940267161452256No Hit
GCGTGGACAACCCCCACCCCCAACACACAGTCAGCTCAGCCAGATTCTAC8390.4775212152601893No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA8290.4718296632308664No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC7760.4416644374754552No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC7640.43483457504026773No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGATCCTG6910.39328624522621075No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGATCCT6790.3864563827910233No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC6600.3756424339353098No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA6430.3659667954854609No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGATC6330.36027524345613804No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC6200.35287622581801836No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC6170.35116876020922144No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC5990.34092396655644025No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG5850.33295579371538825No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC5780.32897170729486225No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT5590.31815775843914873No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG4890.27831689423388867No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA4860.27660942862509175No Hit
AGATGATACTGCTGCCCTCGGTCTGTGTGAAGTCTTAAACCAACTGGAAG4650.26465716936351374No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAT4510.2566889965224617No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG4210.23961434043449306No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA3950.2248163051582536No Hit
CTTTCCCAAAACATGGCACTCACTGTTGAATCCGAGACCTGCACTGAAGG3900.22197052914359217No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG3660.20831080427321727No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA3580.20375756264975897No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC3350.19066699298231635No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGATCC3190.18156050973539975No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT3130.17814557851780602No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT3120.17757642331487372No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGTTGAGGTTTCAATGAATG3060.17416149209728No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC3000.1707465608796863No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT2950.16790078486502485No Hit
CACATCCTCCTAAACACACAGGCTCTTTCAGAATGTTAGTACTGTTTGAA2780.15822514641517596No Hit
CCAATTTTGGTAAGCTGCCCATCTCATGTCCGACCCACTCACCTCCCAAG2770.1576559912122437No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGATC2680.1525335943858531No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT2630.14968781837119163No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA2580.1468420423565302No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG2570.14627288715359793No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTAAGGTTTCAATGAATG2210.12578329984803557No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG2140.12179921342750953No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA2120.12066090302164498No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA2020.11496935099232208No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT1990.11326188538352522No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT1900.10813948855713464No Hit
CTGAAAGCTGTACCATACCTGTCTGGTTTTGGCTGAGGTTTCAATGAATG1870.10643202294833777No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1860.10586286774540549No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGATCC1790.10187878132487949No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1790.10187878132487949No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT1780.10130962612194719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)