FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175699
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT151448.619286393206565No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG139947.964757909834433No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT129397.364299170740869No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT104185.929458904148572No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG80214.565193882719879No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA75014.2692331771950895No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC64863.6915406462188174No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG63563.61755046983762No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG54953.1275078401129206No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA44652.541277981092664No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC44182.514527686554847No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA32811.8673982208208355No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC27631.5725758257019107No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA21451.2208379102897569No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT21231.2083164958252466No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT20491.1661990108082574No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT17440.9926066739139097No Hit
GGATCATTTTCACACTGTCCTTCCTATGTGTGATCCATGGGAGCCAGCCT16990.9669946897819566No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG14950.85088702838377No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT14700.8366581483104627No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG14550.8281208202664784No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG14030.7985247497139994No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA13870.7894182664670828No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA13610.7746202311908434No Hit
GTGTACACAGTGTCCACCAAGCTGCCATGTGTGGGTAGAATCTGGCTGAG12620.7182738661005469No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA10900.6203791711961935No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG10250.5833840830055949No Hit
GGCAAGGACAGGGAAGGGCTTTAAACAGGAAAGCTTCGGGGGCATGTTTC8840.5031331993921423No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT8710.4957341817540225No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT8300.47239881843379866No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG7720.439387816663726No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT7500.42686640219921573No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA7170.4080842805024502No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC6820.38816384839982015No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT6780.38588722758809096No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA6570.37393496832651296No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG6420.36539764028252864No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT6420.36539764028252864No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG6230.3545836914268152No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG6100.34718467378869544No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5980.340354811353508No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA5820.3312483281065914No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG5680.3232801552655394No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT5420.3084821199892999No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG5410.3079129647863676No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA4900.27888604943682094No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA4860.27660942862509175No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4490.25555068611659715No Hit
ACTTTCAAGTTTCCCCACCCTTTCAAGTGTCTTCCAGTTGGTTTAAGACT4300.24473673726088369No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT4120.2344919436081025No Hit
GTGTACTGCCCTCCTCCCACCCTACCCTTCAGTGCAGGTCTCGGATTCAA3960.2253854603611859No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA3840.21855559792599843No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC3530.20091178663509754No Hit
GTTGATACAAAACAAGCCCACGAACTAGCCAAGAGTTACGGGATTCCATT3430.19522023460577465No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT3330.18952868257645178No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC3270.18611375135885805No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG3190.18156050973539975No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGAATCCAGGGTGTCCACCCAATT3150.1792838889236706No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT3130.17814557851780602No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT3070.17473064730021232No Hit
CGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG3000.1707465608796863No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT2970.16903909527088942No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC2740.15594852560344682No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2680.1525335943858531No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT2610.14854950796532707No Hit
TGAGATTGCCTTGAAGTTGTGCCTGTTACTTGGGAGGTGAGTGGGTCGGA2530.14399626634186877No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT2340.1331823174861553No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG2180.12407583423923871No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA2130.12123005822457726No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA2060.11724597180405125No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT1990.11326188538352522No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT1980.11269273018059295No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACTCAATT1980.11269273018059295No Hit
CAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC1950.11098526457179608No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTTCACCCAATT1940.1104161093688638No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAATTGGTGGTG1880.10700117815127007No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1860.10586286774540549No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC1850.10529371254247322No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1790.10187878132487949No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1780.10130962612194719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)