FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411940
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA884106.261597518308143No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA804425.697267589274332No Hit
CATGTGAGTGGAGGAGGATCCATAGATTTGTCTGACACAGACTCCCTACA677764.8002039746731455No Hit
CATGTTATGTGTTTTTCACCACAATAAAAATATGAAGATTGTTACTAGTT603214.272207034293242No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAAATGCATACCCACAAACTGT490353.4728812839072485No Hit
ACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTCCTAG457453.2398685496550845No Hit
GCAGAACATTTTGTTTCCTCACTAAGGTGATGTTCCTGAGATGCCTTTGC422922.995311415499242No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG419302.9696729322775757No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAATATTTCAGAAAAAGACCT411452.9140756689377736No Hit
CACCTCGAACACCCAGGCGAGGTCAGAACAGGAGTGCACGGATAGCAAAA401012.8401348499228014No Hit
CTCCCAAAATTCAAAATCCTCACCTGTACTGTCAAACTCAGTATATTTTT342182.423474085301075No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC308352.183874668895279No Hit
GGGCAGTTGGCCACTTTTACTTATAGCTTTATTTACAAGGAGGTTATCTG293132.0760797201014207No Hit
TTCCAGTTCATTTATCCAAGTTGTTTTTACATTACCATCAATGTCATCAA287772.0381177670439254No Hit
AGTTGTGGTTACCTAGTTATTATGGAAGTGTTTCCACATTTTTATGAACA174881.2385795430400726No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGCTGTGAATTCTCAACAGA167411.1856736121931526No Hit
AAAATTTGTTTATAGTCATCCAAAGTTAAAGAAATTAGAAGAAGTTGTAA153721.0887148179101094No Hit
CACCTCGAACACCCAGGCACACTGACGACATGTTTCTTCTATAACCCACA105570.7476946612462285No Hit
ACTGTGTTTGGTGTTCTTTAGGTAATATTAAATTTTATAAAAGCCAAAAG102200.7238267915067212No Hit
TTCCAGTTCATTTATCCAAGTTGTTACACTGACGACAGGGTTCTATGATT97080.6875646273920988No Hit
TTCCAGTTCATTTATCCAAGTTGTTACACTGACGACATGTTTCTTCTATA67030.47473688683655113No Hit
GCAGAACATTTTGTTTCCTCACTACACTGACGACATGTTTCTTCTATAAC59700.4228224995396405No Hit
CCAGACTCTCCAGAACCAAAGGTGCTGACTACTACCGCTAGACCAAGTCT58120.4116322223323937No Hit
TGATAAATGCACCTGTTTGCTGTACACTGACGACATGTTTCTTCTATAAC52250.3700582177712934No Hit
CTCCCAAAATTCAAAATCCTCACACTGACGACATGTTTCTTCTATAACCC51700.36616286811054294No Hit
CTCCCAAAATTCAAAATCCTCAACACTGACGACATGTTTCTTCTATAACC44200.313044463645764No Hit
CACCTCGAACACCCAGGCCACACTGACGACATGTTTCTTCTATAACCCAC37260.26389223338102186No Hit
CCCCTATTGCATATTTCTTCATGTGACACTGACGACATGTTTCTTCTATA31160.22068926441633496No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC29820.21119877615196114No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAGACCAAGTCTCTGCTACCGTATC29770.21084465345552927No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA28770.2037621995268921No Hit
ACCTTGTCACCAATACCTCACATACACTGACGACATGTTTCTTCTATAAC28240.20000849894471437No Hit
GCCTACCAAAATCAGAGAAGCCATTAAATGAGGAAACAGTGGTAAATAAG27610.1955465529696729No Hit
CCCCTATTGCATATTTCTTCATGTGTACGGTAGCAGCATCTTGAATCTCA24310.1721744550051702No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT23090.16353386121223282No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG22750.16112582687649615No Hit
ACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTCCAGA22700.1607717041800643No Hit
GCAGAACATTTTGTTTCCTCACTACACTGACGACAGGGTTCTATGATTTC22180.15708882813717295No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCAGACCA21700.1536892502514271No Hit
CATGTGAGTGGAGGAGGATCCATAGATCTGTCTGACACAGACTCCCTACA21540.15255605762284516No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT21220.15028967236568125No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA19780.14009093870844372No Hit
GCTTTCACTTCTCTGGCTGAATCTACTTCTTTTATAGTTCTAATTTCAAA19590.13874527246200263No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT19570.13860362338342988No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC18960.1342833264869612No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC18800.13315013385837923No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG18670.1322294148476564No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG18620.13187529215122454No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG18250.12925478419762879No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA18180.1287590124226242No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA17640.12493448730116011No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC17570.12443871552615549No Hit
GGAGTTGAACCCAGCCGTTACCTGTGTGTGAGACCAAGTCTCTGCTACCG17480.12380129467257815No Hit
CTGCACAGTGAATCCAAAAGAAAGTATACTGAAAAGAGTGAAGGATATAG17380.12309304927971443No Hit
CACCTCGAACACCCAGGCGAGGTCAGAACAGGAGTGCACGGAAGACCAAG17230.12203068119041886No Hit
TCAGCTCACTTCAAGAAATCTCCATGTATGCACAAGTAACTCATCAGAAG17210.12188903211184611No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA17120.12125161125826876No Hit
CCCAGCTGAGATCTTACCAGATATATAGCGACTTGGGTTATTCCTAAAGC17020.12054336586540505No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT16880.11955182231539585No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT16850.11933934869753672No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG16830.11919769961896398No Hit
TGTGCCTCCAAACTTACAGGTGAAGTAAATCTAATGTTTTTTAGGTCGTG16560.11728543705823193No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA16460.11657719166536822No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG16340.11572729719393174No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA16190.11466492910463617No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC16000.11331926285819512No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT15990.11324843831890874No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTAG15750.11154864937603581No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG15240.10793659787243083No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT15000.10623680892955792No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA14960.10595351077241243No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT14810.10489114268311685No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT14660.10382877459382125No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT14280.10113744210093914No Hit
AGCACCAAACGTAAAGCAGCATCTTGAATCTCATACAGACCAAGTCTCTG14250.10092496848308002No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC14230.10078331940450728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)