FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411940
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG854896.05471903905265No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC776415.498888054733204No Hit
AAGACATCACTGAAATCATAGAACCCTTACGTTCACTGACTGGACCCATG608464.309389917418587No Hit
AGAAATGATTGGGGAGCTCTTATTCTAGTGGATGATCGCTTTAGGAATAA587924.163916313724379No Hit
CAGGTGGCCCTACCTCAAAATTATTACTATTAGATATGGACAATTTAATG490103.471110670425089No Hit
CCCCTCTCATTCTTTACTACTTCCCTAAAGAGAAAACACACACCTTGATC442163.131577829086222No Hit
CCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCA420612.97895094692409No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC414862.9382268368344264No Hit
GTATGAGATTCAAGATGCTGCTCTTCATCTCTCTTATTTACCACTGTTTC413732.9302236638950663No Hit
TGTTAGGGAGAACTTACATCTAAATCTACTTTAACTGTTTTAAAGAAATC399082.8264657138405314No Hit
TCGTCTAATGTTTTTAACACTGGTATCTCCTTTTATATTAACAGATGAAC328062.323469835828718No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG305132.161069167245067No Hit
AGCGGTAGTAGTCAGCACCTTGAACACATTCCTCCTAAAGGTAGCAGTGA295542.0931484340694366No Hit
GATTGGCTAACAATTCAGATTGCATTTTAGCTAAAGACCTTTTTGAAATT284152.012479283822259No Hit
AAGATGTATCCTATATCCTTCACTCTTTTCAGTATACTTTCTTTTGGATT185971.317123957108659No Hit
TGTTAGGTACTAAATTTTCATTTTGATTATGTTTATGTACATCACAGTCA175191.24077510375795No Hit
CTGCACCCAGCCATAAGTAAAGTTTTACCTCTTGCAATAACTTTGTCTTG133910.9484114055838067No Hit
TGGTTAAGATCATTTGGTAACATTTGTATGTCAAATACATAGTGAATATA114600.8116492202218224No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGCCTGGGTGTTC103420.7324673852996586No Hit
AAGACATCACTGAAATCATAGAACCCTGTCGTCAGTGTAACAACTTGGAT94790.6713458078955197No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTAACAACTTGGAT64910.4597220845078403No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTAGTGAGGAAACA58030.4109948014788164No Hit
AGCGGTAGTAGTCAGCACCTTTGGTTCTGGAGAGTCTGGTGTAGAACCAT57460.40695780273949317No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTACAGCAAACAGG50820.35993030865334225No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGAGGATTTTGAA50200.35553918721758715No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTTGAGGATTTTGA42700.30242078275280815No Hit
AAGACATCACTGAAATCATAGAACCCTTATGTTCACTGACCGGACCCACG38750.2744450897346913No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGGCCTGGGTGTT36220.2565264812952392No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTCACATGAAGAAA30420.21544824850914346No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA29670.21013640806266556No Hit
CCCTGCTTCCAACACTTGTTATTTGGTGTAGAACCATGTCGTCAGTGTAG29640.20992393444480645No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTATGTGAGGTATT27700.19618397382325028No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG25640.18159411873025766No Hit
GTATGAGATTCAAGATGCTGCTACCGTACACATGAAGAAATATGCAATAG24330.17231610408374293No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC23250.1646670538408148No Hit
GGAAACTTGTAAGGGCTTCGAGGAATGTGAGGTATTGGTGACAAGGTTGT22610.16013428332648696No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT22040.15609728458716376No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT21830.15460996926214995No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGTGTAGA21600.1529810048585634No Hit
AAGACATCACTGAAATCATAGAACCCTGTCGTCAGTGTAGTGAGGAAACA21600.1529810048585634No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT21040.14901483065852655No Hit
AAGACATCACTGAAATCATAGAACCCTTATGTTCACTGACCGGACCCACA20780.14717339263708087No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA19420.13754125529413433No Hit
TCATTTACAGTTTGTGGGTATGCATTTGCATCTTTTACATTGGATATTAC19360.13711630805841607No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG18980.13442497556553396No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG18960.1342833264869612No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG18310.12967973143334702No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA18030.1276966443333286No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA17930.1269883989404649No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT17900.1267759253226058No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC17790.1259968553904557No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA17460.12365964559400541No Hit
CACACACAGGTAACGGCTGGGTTCAACTCCTGTAGAACCATGTCGTCAGT17220.12195985665113247No Hit
TCCGTGCACTCCTGTTCTGACCTCGCCTGGGTGTTCGAGGTGTGTAGAAC17110.12118078671898239No Hit
GGCTAGAAATACGTGGCAAAGAATTCTCTGAAGTAAGAAAATCTTTCTTT16700.11827698060824116No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG16610.11763955975466381No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG16580.11742708613680468No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA16490.11678966528322733No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA16490.11678966528322733No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT16230.11494822726178167No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG16180.11459410456534981No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC15770.11169029845460857No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC15640.11076957944388573No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA15210.10772412425457173No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA14960.10595351077241243No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT14790.1047494936045441No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA14460.10241228380809383No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT14400.10198733657237559No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT14160.10028754762950268No Hit
GTATGAGATTCAAGATGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTC14150.1002167230902163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)