FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139098
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA97366.999381730866008No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA79535.717551654229392No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC74145.330055069087981No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC64864.662899538454902No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC59564.281873211692476No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA58224.185538253605372No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC51393.6945175344002075No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC48713.501847618225999No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT46263.3257128067980846No Hit
TCAAGCAATTTAGCAGTTTCAGGACACTGACGACATGGTTCTACATCAAT40822.934621633668349No Hit
TTGCATAAAAATTAACAGCAAAAATGCCTGGTAGAAGACTTCCTCCAGAC40162.887173072222462No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA35672.5643790708708964No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA34392.4723576183697826No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC24221.7412184215445226No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA23841.7138995528332541No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA17801.279673323843621No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAATG17301.2437274439603732No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA13430.9655063336640355No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC13080.9403442177457619No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC12710.9137442666321586No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC12690.9123064314368287No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAATGGAGC10990.7900904398337862No Hit
GAAGTTCATCCTGTTTTAAGTCACACTGACGACATGGTTCTACAGGGAAG10790.7757120878804871No Hit
GGGGTCAGAGGCAAGCAGCTGGGGAGATGTGGGGGTTGTTGACTTGTAGA10750.7728364174898273No Hit
AAGGGAAAAGGATGGATTAGGGTACGGTAGCACAGCAAGTGGAAAATCTG10330.7426418783878992No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCAAGGTAATTAAA10260.7376094552042445No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA10250.7368905376065795No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10240.7361716200089146No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAATGG10100.7261067736416051No Hit
TTTCCTACTGTGGTTGCTTCCAACACTGACGACATGGTTCTACATGGGTT9690.6966311521373421No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAT9300.6685933658284088No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAATGGAG9220.6628420250470891No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCGTTTGCT9170.6592474370587643No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA9060.6513393434844498No Hit
TGTAGATACCTCTGAAGAAGATAGTTTTTCCCAGTTCCAGTTCAAGACCA8590.6175502163941968No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA8220.5909502652805936No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT7920.5693827373506449No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT7300.5248098462954176No Hit
TCAATCATTCTCTACAGCCCAGACCAAGTCTCTGCTACCGTAATGGAGCA7180.5161828351234381No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC6950.4996477303771442No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG5750.4133776186573495No Hit
CTAAATACGTTTCACAGGTAGAAAAACCCAGTACGTCACAGTGTAGACCA5740.41265870105968455No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC5570.40043710189938025No Hit
GTGCTCAAAAGAAGTGCTGAAGGTACGGTAGCAGAAGCCTTTTTAGCCCT5210.3745560683834419No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAATG5110.3673668924067923No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT4500.32351291894923007No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG4040.2904427094566421No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG4020.28900487426131216No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3900.2803778630893327No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT3860.2775021926986729No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAATGG3490.2509022415850695No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA3470.2494644063897396No Hit
CTCCTCCACAGCTCGCTACCGTAAAATCGGACATCTCCTTGAATATAGAC3370.24227523041309004No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC3360.2415563128154251No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTCCTCAGA3100.22286445527613624No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTGAGAC2960.21279960890882685No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAAAATGAAATACTTTGAGA2880.2070482681275072No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA2790.2005780097485226No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAATGGAGCACTATC2790.2005780097485226No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG2670.19195099857654316No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATTCCTGCCATCA2500.17972939941623892No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG2440.17541589383024916No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAATGGAG2380.17110238824425944No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAT2260.16247537707227996No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT2200.15816187148629024No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA2170.15600511869329536No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAATGGA1860.13371867316568176No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAAT1860.13371867316568176No Hit
CCTACTTCCAAGGATGTTCTGTCTTAGCAGAGCTGTTGCCACCTGAAAAA1840.13228083797035184No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT1820.13084300277502192No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC1790.12868624998202707No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAATGGA1770.12724841478669716No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCAAGGTAATTAAATG1680.12077815640771256No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC1620.11646465082172282No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT1600.1150268156263929No Hit
TTACCCAGTACCATCAATGCTGTTAATGAGAGAAGTTTCTGAGAGGTAGA1560.11215114523573308No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG1490.10711872205207838No Hit
CTGACTTATGGTATCAAATAATAAAATTAGAGGAGACCAAGTCTCTGCTA1450.10424305166141856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)