FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139098
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA93566.726193043753324No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG74605.3631252785805685No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG67094.823218162734187No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG64474.634861752145969No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA59344.266057024543847No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG58514.206386863937656No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA51123.675106759263253No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA48313.473090914319401No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC45843.295518267696157No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTCCTGAAAC40482.9101784353477407No Hit
GGAGGAAGTCTTCTACCAGGCATTTTTGCTGTTAATTTTTATGCAATGTA39882.867043379487843No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT35092.5226818502063293No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT34222.4601360192094783No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG23771.7088671296495994No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT21701.5600511869329539No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT17731.2746409006599664No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA17081.2279112568117443No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT13480.9691009216523602No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG13030.9367496297574373No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG12720.9144631842298235No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG12660.9101496786438339No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC10780.7749931702828222No Hit
ACAAGTCAACAACCCCCACATCTCCCCAGCTGCTTGCCTCTGACCCCTGT10750.7728364174898273No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTGACTTAA10350.7440797135832291No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA10270.7383283728019094No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10190.7325770320205898No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT10070.7239500208486104No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTTGGA9550.6865663057700326No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG9300.6685933658284088No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT9210.6621231074494242No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTACCCTAATCCATCCTTTTCCCT9160.6585285194610994No Hit
TGCACGTACCACATTTAATTACCTTGAGACTTGGTCTTCTCTTGGTATTA9140.6570906842657696No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT8970.6448690851054653No Hit
GAGTCCTCCTTCTGTGAGCAAACGAGACTTGGTCTGAGTCCTCCTTCTGT8740.6283339803591712No Hit
TGAACTGGAACTGGGAAAAACTATCTTCTTCAGAGGTATCTACATGTAGA8460.6082042876245525No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA8240.5923881004759235No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT7900.5679449021553149No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT7260.5219341759047578No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTAGATCTCG7200.5176206703187681No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT6310.453637004126587No Hit
ACACTGTGACGTACTGGGTTTTTCTACCTGTGAAACGTATTTAGTGTAGA5730.41193978346201965No Hit
CGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG5610.4033127722900401No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT5560.39971818430171535No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG5550.3989992667040504No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTACCTTCAGCACTTCTTTTGAGCAC5140.3695236451997872No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA5030.36161555162547265No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG4470.32135616615623513No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4240.3048210614099412No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT4050.29116162705430704No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC4020.28900487426131216No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3900.2803778630893327No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT3870.27822111029633784No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3450.2480265711944097No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG3430.2465887359990798No Hit
ATATTCAAGGAGATGTCCGATTTTACGGTAGCGAGCTGTGGAGGAGTGTA3340.2401184776200952No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA3310.23796172482710032No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTATCTCG3110.22358337287380123No Hit
CAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2990.21495636170182175No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT2880.2070482681275072No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAAGAACACAGAGGAGAATTTATT2860.2056104329321773No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTGCAGTTCTTTT2810.20201584494385252No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT2770.19914017455319272No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC2660.1912320809788782No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC2430.1746969762325842No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2330.16750780025593465No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG2200.15816187148629024No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT2160.1552862010956304No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT2110.15169161310730564No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG1920.13803217875167148No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT1830.13156192037268688No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA1820.13084300277502192No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1800.12940516757969203No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1790.12868624998202707No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT1760.12652949718903217No Hit
CGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT1660.11934032121238264No Hit
TGCACGTACCACATTTAATTACCTTGACTTGGTCTTCTCTTGGTATTAGT1610.11574573322405786No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG1590.11430789802872794No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT1590.11430789802872794No Hit
ATTTTTCAGGTGGCAACAGCTCTGCTAAGACAGAACATCCTTGGAAGTAG1560.11215114523573308No Hit
ACCTCTCAGAAACTTCTCTCATTAACAGCATTGATGGTACTGGGTAATGT1540.11071331004040316No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC1520.10927547484507326No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTAGAACCATGTCGTC1500.10783763964974334No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCGTCAACCA1460.10496196925908352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)