FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693130
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT113641.6395192820971534No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA108061.559014903409173No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG103711.4962561135717687No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG101111.458745112749412No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT97701.4095479924400907No Hit
ACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTATTTCATCATTA92681.3371229062369252No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA88271.273498477919005No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA84481.218818980566416No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC83341.2023718494366136No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA81361.1738057795795882No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG79401.1455282558827349No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG77681.120713286107945No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG77021.11119126282227No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC73211.056223219309509No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT72661.0482881999047797No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT70331.0146725722447447No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG70121.0116428375629392No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG69280.9995238988357161No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT67930.980047033024108No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT66330.9569633402103501No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT64640.9325811896758183No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT63040.9094974968620604No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA61470.8868466232885606No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT60650.8750162307215096No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT59940.8647728420354045No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT59880.8639072035548886No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA59780.8624644727540287No Hit
AGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCG58980.8509226263471498No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT58720.8471715262649141No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA58510.8441417915831084No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT56730.8184611833278028No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC56700.8180283640875449No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT55380.7989843175161947No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA55310.7979744059555929No Hit
GTCAAATTGTGTTTCTGCAGCTCTACTTACACGTTCCTCTCGTCCTTCAG53360.7698411553388254No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA52570.7584435820120323No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTAGCACACCCCAACT51940.7493543779666152No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG51620.7447376394038636No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT51180.7383896238800802No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTACCGTAAGGTTTGTGCTCTGGA51010.7359369815186184No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA50450.7278576890338032No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA50310.7258378659125994No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA49170.7093907347827969No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT48860.7049182693001312No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA48550.7004458038174657No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT47220.6812574841660295No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG46520.6711583685600103No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGCAGAATTTATTTCAGTA44250.6384083793804913No Hit
TGCACGTACCACATTTAATTACCTTGACTTGGTCTTCTCTTGGTATTAGT43210.6234039790515488No Hit
CCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTA42230.6092652172031221No Hit
GGCCAGGATATAGTCTAGTTAGTCAAGAAAAACAATGAATCGTTTGCAAA41890.6043599324801985No Hit
CCCTCTCTGCTTCTCCTCCTCCTGCAGGCTTGGCCTTGATGATGCAGCTG40810.5887784398309119No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC40560.5851716128287623No Hit
TACTGTAGGGGCTCGGTGACCGGGTGCTGGACTGGTAGGCCGAAGGCTCC39200.565550473937068No Hit
CAGAACTGTTGCCATTGTGTCAGAATCCTTCAGGTTCCTGTTCCGTAGAT38670.5579040006925108No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT37360.5390042272012465No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG36790.5307806616363453No Hit
TGCAATTTGGTCTCTGCATGTACCACAAAATTGCTGCTTCCCTCAGCCTT36640.5286165654350554No Hit
GTTATCAGCTTGTCAAGAATAGCAGTAACTCTTTGTCGTTTGGCATCATC36240.522845642231616No Hit
GATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGC35460.511592341984909No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG35450.511448068904823No Hit
GGAAAAACCAAGTGTGGGAGGCCCCAGTCCTCTCTGCCAATCCGCTATAC34510.4978863993767403No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA34340.4954337570152785No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTACTCATAACTTTTTTTTGCACT33680.48591173372960333No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA33490.4831705452079696No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG32480.4685989641192849No Hit
GGGAACTATTCCACATGGGTCAGGAATTCCCAGCTCTTACTTCAAAATTA32180.46427077171670533No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC31140.4492663713877627No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT31070.44825645982716084No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT30280.43685888650036786No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG30200.43570470185967997No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG30110.4344062441389061No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC30090.4341176979787341No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG29940.4319536017774443No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT29750.42921241325581055No Hit
GTGGACCCATTACATTAGATTTTTCCAATCAAAGGATACTTTTGACCTAC29550.42632695165409085No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTAGTGGTG29450.42488422085323096No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG28510.4113225513251482No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC28360.4091584551238584No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG27830.4015119818793012No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG26780.3863633084702725No Hit
TCCAAATGGAGTGTTGCTGAGGTCTCAGATGGGCACAAATGCAAATTACT26770.38621903539018654No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA26720.3854976699897566No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG26600.3837663930287248No Hit
TGAAAGAAAGTAACATTCAACACTGATACGGTAGCAGAACTTGACTGTTG26400.380880931427005No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT26210.3781397429053713No Hit
TTCAATTACCAGTTTATTTATGAAATCCCTAAATGTCATAAAAATGGTTT26130.3769855582646834No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG26060.37597564670408146No Hit
AATAGCAACAAGAAAAGATGGAAGAGTACTAAATTTGAGTAATCTAGGAA25450.3671749888188363No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT24860.35866287709376304No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT24720.35664305397255924No Hit
ACTGTAATAAATGGAAAAACATTTTACTTACCTTTATATCACTACCTGGT24550.3541904116110975No Hit
CTCTGACTCCACCTTTCCACTTGCCCAGTATTGTTGGTGTTTTTCTTCTT24470.3530362269704096No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG22790.3287983495159638No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT22150.31956487239046066No Hit
ATGTGTCCCAGCTAAATGTGGTCTTGACTTGGAACAGGATGCCCCTGAAG21610.3117741260658174No Hit
CAGCCATATCAGTCTGTGGGATCTGGTATAGTTTTGTAAATTCATCTGGA21580.3113413068255594No Hit
CGATAAAAGTGAACCTATTAATCTCTATAGTCTTTTTGTTTATAAAGGAT20540.29633690649661676No Hit
ATAATATTCCTTGAGTTTACATTAACTTACCAGAAGCTTGTTTCCTGTAC19970.28811334093171553No Hit
AGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAG19820.2859492447304257No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG19530.28176532540793214No Hit
TGGCTGTAATTTGGTTAGTGCTATTACTGTAGCTTCTATCAGAACTTGAC17870.2578159941136583No Hit
TCACTTTTTCTTCCTGTACACCTCTACGGTAGCAGACTATGGTTTAGGAG17760.25622899023271245No Hit
CAGGACTAGCTCTAAGAAACCTGTGATACCAAACTGGACATTACGAGCCT17220.24843824390806918No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG17210.24829397082798318No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG17150.24742833234746728No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG17150.24742833234746728No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT17100.24670696694703734No Hit
AGCAATTTCAACAGTCTAATCAATGTCGTCAGTGTACTTCTAGGCACATG16350.23588648594058834No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA16110.23242393201852465No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT15900.22939419733671892No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCATC15430.22261336257267755No Hit
TGTCTGTTTTGTTGGGTTTTGTTACTATTTTGTGACTTATTTTTCTTCTT15390.2220362702523336No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA15120.21814089709001194No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC14910.21511116240820624No Hit
GAACGCTTTGAACTTCCCGAGGTAAGCAAGCAGGACATGTAGAACCATGT14810.21366843160734636No Hit
TTCTTACCTCCGTGCAACATAATAAAAAATAGGATTCCCAGCTTTGGAAG14560.21006160460519668No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG14560.21006160460519668No Hit
CCATGGGACTGACTTTCTGCTACCGTATCACACAGAGCCCTGAACAAATA14450.20847460072425084No Hit
TCCTCAGCAGTCTGGAACCCCGCAGACACCAGCTTCACCCGCACCGCTGG14170.2044349544818432No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC13720.19794266587797385No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT13530.19520147735634008No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA13510.1949129311961681No Hit
GGAAACTTGTAAGGGCTTCGAGGAATGTGAGGTATTGGTGACAAGGTAGG13140.1895748272329866No Hit
CCAGAGAGGTTGTAACTTACCTTTTCCAGGCTATTTATAACTGCTAACTG12760.1840924501897191No Hit
TCAGCACCATTCAACCTTTGTACGGTAGCAGCAAGTCATCTATCACCATG12690.1830825386291172No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT12510.18048562318756942No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC12280.17716734234559173No Hit
ATCTTACCTTCAACAGAATGACAGGATACGGTAGCAGACCTTTGTGCTTT12220.17630170386507582No Hit
GAGACCTACATCAGGCCTTCATCCTGAGGATTTTATCAAGAAAGCAGATT12130.17500324614430193No Hit
GAGGGGGTCGTGGGACTGGTCTCTGGGCGCGGGAATCTGTAGAACCATGT12070.174137607663786No Hit
TCACAGCCATTAAGAGGACAAACTTACGGTAGCAAATACAACAGCAAATC11970.17269487686292614No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC11630.16778959214000258No Hit
CTGCCTTTAACAAATGTTTTCTCATTATTTCAGTTGCCAAAGCTGTTTGA11520.16620258825905673No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC11490.16576976901879878No Hit
TTAGTTATCGTTTCTTTCCTTTCTTACAGCCCCCTGGCCACTGTTCTAGG11430.16490413053828284No Hit
ATCTTACCTTCAACAGAATGACAGGATTTATGCAGATACATTGGACCACT10870.15682483805346759No Hit
GGCTAGAAATACGTGGCAAAGAACCATGTCGTCAGTGTCGGTACAACTTC10790.1556706534127797No Hit
CAGCTGCCTACTTCCTCCATGGCCAGCAAGAGCTTTTCGTAGACTGTCCA10640.1535065572114899No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG10150.14643717628727654No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT10120.14600435704701858No Hit
TTCCCTGTGCTTTCAAATCTTTAACAAGATGCTTCTCGGAGTTCTCATCC10100.14571581088684663No Hit
TCATTCAGAAAACAAACAGAGCACATAAAATGATACAGAAAAAGTACAGA10030.1447058993262447No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT9980.14398453392581478No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC9870.1423975300448689No Hit
TTCATGGTGTTTTATCCCTCTTGACTCTTTGAACAATGTAGAACCATGTC9850.14210898388469695No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGAGACTTGGTCTGTCTAAGAACA9560.13792506456220333No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA9510.13720369916177338No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT9420.13590524144099952No Hit
CGTCTTCCCGCGCATCGCAGGCTCACCTGCTAACCCCGGAGCAGCCAAAC8710.12566185275489444No Hit
AGTCATGTTGATCCATTCCTGTAGGGAGTCTGTGTCAGACAAATCTATGG8590.12393057579386262No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA8550.12335348347351867No Hit
CCATGGGACTGACTTTCTGCTACCGTAGGTTGGAAAGCCATAGGATTCCT8540.12320921039343267No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT8260.11916956415102505No Hit
ACCAGAATCTAAAACATTTCCAGCAAGTGAACTAGTGGATTTATTTGTAA8120.11714974102982123No Hit
GACTCACCACTTCACGATGCAGCCAAGAATGGGCACATGGATATAGTCAA8110.11700546794973525No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG8090.11671692178956328No Hit
GATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCT7970.11498564482853144No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT7780.11224445630689768No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT7720.11137881782638176No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT7720.11137881782638176No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT7530.10863762930474802No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA7380.10647353310345821No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA7040.10156824838053466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)