FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961435
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT349383.6339430122681198No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA291213.028909910706392No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC283202.945596946231414No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG279822.9104411634691894No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA251932.6203539500850295No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC185271.9270153468513214No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT175321.8235242112051255No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA174111.8109388570210154No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC160671.6711478155049482No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC157731.6405685251733086No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT157571.6389043461076411No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT150881.5693208589244203No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG126981.3207341109903425No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT111721.162013032602308No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC108821.1318497870370852No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA107571.118848388086558No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT102051.0614342103210306No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA101271.0533213373759016No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC98791.0275265618580558No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG98231.02170193512822No Hit
GTATAATGATGAGCATTTTGGCTACATTACCCACCAACATGGGAGGATTA95440.9926828126706434No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC95120.9893544545393084No Hit
TTCTTCATGGAGCAGAACTGGTGGGCTCTCCTGATGCCTGTACACCTCTT93430.9717765631581958No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA91880.9556548284595422No Hit
AGAAGACCTACCAAATGATAAAAGGACATCAGATACAGATGAAATTGCTG91310.9497261905381018No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA89560.9315242320073639No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC86680.9015690088253496No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA84890.8829510055281948No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA84440.8782705019060051No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT84010.8737980206670237No Hit
CCTCTGAAAGTGGACTGGAAAAGGAATACAGTTGGCTAGACCAAGTCTCT82520.8583003531179955No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC80480.8370820700307353No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC75790.7883008211683578No Hit
AGACATCGCTGAATTTAAAGGATACAATTTCCGAAGTCTCAGACTTCAAA68940.7170531549194693No Hit
AAAAATGAACTTGGCCGAAATGAAGACTTCATGAAACTCTATAATCATCT66430.6909463458268109No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA65040.6764887901938249No Hit
TGAATGTAGCACGCATTCACATAAGGTTTTTGCTGACATTCAGAGTGAAG64050.6661916822250074No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC63690.6624472793272557No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC62810.6532942944660846No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG60490.6291636980139063No Hit
GGGAAGCTCTTCATCCTCACTAGATAAGTTCTCTTCTGAGGACTCTAATT59200.6157462542969624No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA55270.5748698559965053No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA53450.5559398191245378No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAT53380.5552117407833083No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA51220.5327453233967975No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACATA51160.5321212562471722No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG50990.5303530659899005No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC50940.5298330100318794No Hit
GTTGCCTTATTAACGGTATCTTCAGAAGAATCAGATCCTAAAAAATTTCC50540.5256725623677108No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC50420.5244244280684601No Hit
GCCGGAACAGCCCCAGCCCTAAATTTCAGATTAGCCTTAGGGGTTGCCTT50140.5215121147035421No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACATACCT49730.5172476558477692No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC48720.5067425254957433No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC47980.49904569731703136No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA47490.4939491489284247No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG45930.47772340303816696No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT45180.4699225636678506No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG44470.4625377690639513No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC44270.46045754523186694No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG43410.4515125827539043No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG42950.4467280679401104No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG41680.4335186466063749No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT41420.43081435562466525No Hit
CTCCTTTCTGGACGCTTTTGCTAAAAACAGCAGAACTTTCCTTAATGTCA40730.4236375834039742No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT40120.4172929007161171No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT39650.41240437471071884No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA38290.3982588526525454No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACA38160.3969067071616906No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT37930.3945144497547936No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT37820.3933703266471473No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT37630.3913941140066672No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA35900.37340017785913765No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA35760.37194402117667863No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT34990.3639351594231539No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG33590.3493735925985636No Hit
AAACTTAAGGTTTTGATGGCCTACCGACTGGAAAAGGAATACAGTTGGCT33470.348125458299313No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA33080.34406902182674853No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG32410.337100271989266No Hit
GGCCTGCCCAGGTTGTGGGAGCCCACTTCATTAGTACTGGAGACCAAGTC32220.3351240593487859No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT32070.3335638914747227No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT31280.32534700733798955No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACAT30430.3165060560516312No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA29900.3109934628966077No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG29660.3084971942981065No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT29490.3067290040408348No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACATAC29230.30402471305912515No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG28960.3012164108858113No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA28810.29965624301174804No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC28460.2960158513056005No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG27850.2896711686177433No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA27540.28644682167801255No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG27410.2850946761871578No Hit
TTGCATAAAAATTAACAGCAAAAATGCAGAGTCTTTTCAGTTTCACACTG26330.27386146749390233No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG25790.26824486314727464No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA24970.25971594543572885No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA24100.25066697176616204No Hit
TCTTGCAAAAACATCCACTCTGTACGGTAGCAAAGGCCAATCAAGTGAAG23900.24858674793407773No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACATACC23900.24858674793407773No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT23110.2403698637973446No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACCACATTTAGCTGGGACACATAG22400.2329850691934452No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT22320.2321529796606115No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC22300.23194495727740302No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACATA22040.2292406662956934No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC21660.2252882410147332No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG21600.2246641738651079No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT21040.21883954713527173No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACATACC20800.21634327853677057No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG20120.20927051750768383No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT19730.20521408103511937No Hit
CCCCTATTGCATATTTCTTCATGTGTACGGTAGCAGCATCTTGAATCTCA19690.2047980362687025No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC18840.1959570849823441No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA17710.18420382033106764No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACATACCTG17680.18389178675625498No Hit
GCCGGAACAGCCCCAGTGCCCCCTCCTATGGGAAGCCAAATCACAGTTAT17640.1834757419898381No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC17280.1797313390920863No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA16730.1740107235538544No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG16650.17317863402102068No Hit
TCAATGCAGAGGTTGAAGATGGTTACCAGAAGTTTTTCTTTTTAGACCAA16600.17265857806299958No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT16220.16870615278203935No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT16010.1665219177583508No Hit
ATACCACTGACGGCCAGGTACACTGACGACATGGTTCTACTACCCTTTCT15740.16371361558503694No Hit
GTCTCGGAGGCTGGCATCTTCTGCTTCTGTTTCAAAAGACCAAGTCTCTG15730.16360960439343275No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT15680.16308954843541165No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC15500.16121734698653575No Hit
ACTTTCCTCTGGCAATTGGACATTTGGAAAGCTTTGAGGCCTTTGCATCC15180.15788898885520083No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG15120.15726492170557552No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC14880.15476865310707433No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT14790.15383255238263638No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACATACCTGATATC14670.15258441808338577No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA13970.14530363467109061No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC13490.1403110974740882No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCCCTAGACCAAGTCTCTGCTA13380.13916697436644185No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACATAC13330.13864691840842075No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA13150.13677471695954485No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG13050.13573460504350268No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG12630.13136613499612557No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC12520.1302220118884792No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGAAGGTAATTAAATGTG12110.12595755303270634No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG11710.12179710536853765No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG11690.12158908298532922No Hit
TCAAGCAATTTAGCAGTTTCAGGAAGCAGATATCCGGTGTAGACCAAGTC11350.11805270247078586No Hit
TCGAATCAAGCACTAATACCAAGAGTACGGTAGCAGAAACTACCTGCTGC11220.11670055697993104No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACATAC10800.11233208693255395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)