FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961435
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT323963.3695465632102017No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG277662.8879747460826786No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT271152.8202634603483334No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG269082.7987331436862606No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT247682.576149193653237No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG182951.902884750399143No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT171321.7819197345634392No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA170351.7718306489778302No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA158791.6515937114833557No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT156241.6250708576242803No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA155221.6144617160806503No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC147091.5299006173064222No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC125041.3005559398191247No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG124471.2946273018976842No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG117831.2255638706724843No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA107531.118432343320141No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA105571.0980461497657148No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC100341.0436482965567095No Hit
AGGAAAAGGTCTAGGGTCAGGAAAGAATCCAAGTTTGGTATACCAGCGAG98861.0282546401992854No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG97451.013589062183091No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC96241.0010037079989809No Hit
GTGTCAATGTACACCACAGCTCCTTCTAATCCTCCCATGTTGGTGGGTAA93160.9689682609848821No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT92640.9635596790214628No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA92080.9577350522916266No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT90920.9456697540655374No Hit
GTGGCAGCAATTTCATCTGTATCTGATGTCCTTTTATCATTTGGTAGGTC90680.9431734854670363No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA86690.9016730200169538No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC83780.8714057632601268No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT83130.8646450358058527No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG82670.8598605209920587No Hit
AGCCAACTGTATTCCTTTTCCAGTCCACTTTCAGAGGTGTAGAACCATGT81190.8444668646346347No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA80580.8381221819467775No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA78970.8213763800984986No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG76240.7929813247905475No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT73930.7689547395299734No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC72410.7531450384061324No Hit
GGTGTGGTAGACTGGACTTCATTATGATTCTGAGGTTTGAAGTCTGAGAC67980.7070680805254645No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG62900.6542303951905225No Hit
TGTACGTGCAAACATACACTCTAGATGATTATAGAGTTTCATGAAGTCTT62450.6495498915683328No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG62410.6491338468019159No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC59820.6221949481764238No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA53290.5542756400588703No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA52850.5496991476282848No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT52660.5477229349878047No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC51160.5321212562471722No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT50980.5302490547982963No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT50670.5270247078585656No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC49310.5128791858003922No Hit
GGCAAGGACAGGGAAGGGCAGGAAAAGGCAACCCCTAAGGCTAATCTGAA49060.5102789060102867No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA47080.48968469007265186No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT46990.48874858934821386No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA46900.48781248862377596No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG45490.47314691060758135No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA44930.4673222838777452No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA44290.4606655676150754No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT43230.4496403813050285No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA42400.4410074524018784No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA42240.439343273336211No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT41310.4296702325170188No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG40560.4218693931467026No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT40250.4186450462069719No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAATGACATTAAGGAAAG39590.4117803075610936No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT39180.40751584870532065No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT38670.40221127793350564No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT37870.39389038260516834No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG37750.39264224830591776No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT37150.3864015768096647No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC36720.3819290955706834No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA35210.3662234056384467No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT34740.3613348796330485No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC34340.3571744319688798No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC34090.3545741521787744No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG33750.3510377716642311No Hit
AGCCAACTGTATTCCTTTTCCAGTCGGTAGGCCATCAAAACCTTAAGTTT32760.3407406636954136No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG32270.33564411530680704No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC32050.3333558690915142No Hit
CCAGTACTAATGAAGTGGGCTCCCACAACCTGGGCAGGCCTGTAGAACCA31740.33013152215178354No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA30210.3142178098363384No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG29670.3086012054897107No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT29250.3042327354423336No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC28920.3008003661193945No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG28550.29695195203003844No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA27820.28935913504293065No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT27680.2879029783604716No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC27620.2872789112108463No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT27060.2814542844810101No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC26710.27781389277486257No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG26150.27198926604502643No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT25240.26252424760904275No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA24860.2585718223280825No Hit
AGCCAACTGTATTCCTTTTCCAGTCCATAAACTTTCCTTACCAAAATAAT24740.2573236880288319No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT24720.2571156656456235No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT23800.24754663601803556No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA23180.2410979421385741No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTACAGAGTGGATGTTTTTGCAA22870.2378735951988434No Hit
ATGTGTCCCAGCTAAATGTGGTCAGTGTACTCCAAAGCCTCTTGCTCATG22100.2298647334453187No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG22050.22934467748729764No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG21990.22872061033767233No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT21940.22820055437965123No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA21580.22445615148189946No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA21220.22071174858414766No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG21000.2184235023688549No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT20780.2161352561535621No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA20700.2153031666207284No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT19880.2067742489091826No Hit
GTATGAGATTCAAGATGCTGCTACCGTACACATGAAGAAATATGCAATAG19480.20261380124501396No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC18920.19678917451517783No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA18690.19439691710828086No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA17470.18170755173256642No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG17420.18118749577454535No Hit
CTTAAGAAATAACTGTGATTTGGCTTCCCATAGGAGGGGGCACTGGGGCT17130.17817117121802306No Hit
AAAAAGAAAAACTTCTGGTAACCATCTTCAACCTCTGCATTGATGTAGAA16380.1703703318477068No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC16020.166625928949955No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT15930.16568982822551706No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT15840.1647537275010791No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT15740.16371361558503694No Hit
TTTGAAACAGAAGCAGAAGATGCCAGCCTCCGAGACTGTAGAACCATGTC15520.1614253693697442No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCTGGCCGTCA15500.16121734698653575No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT15300.15913712315445142No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA14990.15591277621472072No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14980.15580876502311647No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG14950.15549673144830384No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA14870.1546646419154701No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC14810.15404057476584482No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT14720.15310447404140684No Hit
AGGGATGCAAAGGCCTCAAAGCTTTCCAAATGTCCAATTGCCAGAGGAAA14090.14655176897034122No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT13860.14415951156344423No Hit
AGGGGTGGTAAAAAGATAACCTCAGAATAAGAATGTAGAACCATGTCGTC13300.1383348848336081No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC13240.1377108176839828No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA12960.13479850431906473No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC12610.13115811261291715No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC12480.12980596712206235No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG12430.12928591116404126No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA12090.1257495306494979No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT11640.12106902702730814No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC11570.12034094868607861No Hit
ACACCGGATATCTGCTTCCTGAAACTGCTAAATTGCTTGATGTAGAACCA11290.11742863532116056No Hit
TGCACGTACCACATTTAATTACCTTCTTGGTCTTCTCTTGGTATTAGTGC11150.11597247863870151No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTACTCTTGGTATTAGTGCTTGAT11020.1146203331478467No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC10740.11170801978292864No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT10330.10744356092715576No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA10030.10432322517902927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)