FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301284
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA249858.292839978226525No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT171765.700933338643938No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC159305.287370056159637No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA157525.228289587233308No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC115953.84852829888079No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA95363.1651199532666854No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC87452.9025769705659776No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA86742.8790111655448016No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA81352.7001101950319297No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT81002.688493248894731No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA62262.066488761434394No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC58331.9360470519509831No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA55091.8285073219951937No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG54971.8245243690338684No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT47631.580900412899457No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG44471.4760159849178847No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC40921.3581869598120047No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG40611.3478976646619136No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG39661.3163659537180865No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG34841.1563840097715112No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA30761.0209636090864433No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG28620.9499342812761382No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT27940.9273642144952935No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA27240.9041303222208946No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG25480.8457136787881202No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT23000.7633993175873927No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCGTAGGGACGAACTGGTGTAA20840.6917061642835332No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA19740.6551957621380491No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAGAACGTGGGAGTAGACGGAAG17810.5911366020100636No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGCTCGTAGTAT17700.5874855617955153No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA17200.5708899244566589No Hit
TCAAATCATCCATTGCTTGGTGGTAGCAGAAAGTCAGTCCCATGGAGACC17150.5692303607227732No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16020.5317242203369578No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14960.49654146917858233No Hit
TCTTAAATGGCTACGACCCAGTGGTTCTACATCTTAAATGGCTACCTGTT14530.4822692210671659No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG14050.4663374092218638No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT14010.46500975823475527No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT12050.3999548598664383No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG11450.3800400950598107No Hit
TGTTCATTCATGATCCCACTGCTACCGACAAGTGTGAGTGACTGGTGGAG11280.37439757836459947No Hit
GTGTCACTCGTAATTAGGTCCAGACGCTAATGAGTTTGTACTGAGGCCAG11120.3690869744161655No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC10890.3614529812402915No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT10640.3531551625708634No Hit
CCCCTGCTCCTATAGCCAAGTCTCGATGCCGACGAGTACCTCATAGACCA10570.35083177334342347No Hit
GGGGTCAGAGGCAAGCAGGGGGCAGGAGGGGAGATAACACAGGCCCAAGA9660.32062771338670487No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC9460.31398945845116233No Hit
CCCCTGCTCCTATAGCCAAGTCTCAGTGGATGCCGACGAGTACCTCATAG9380.31133415647694535No Hit
CAGAGGGGAAAAATATGACAAAGAAATCTATATAAGATATTATTTTATTT8390.27847479454600976No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGC7670.2545770767780566No Hit
CCCCTGCTCCTATAGCCAAGTCTCAGTGTATGCCGACGAGTACCTCATAG7480.24827073458929116No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGGGAGGTGGCTTTAGGTCAGC6960.23101127175688055No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC6890.22868788252944067No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC6690.2220496275938981No Hit
GTGTCACTCGTAATTAGGTCCAGCTGCTACCGTAGCTAATGAGTTTGTAC5720.18985409115651677No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG5490.18222009798064284No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT5300.17591375579187743No Hit
GGTCTTCTCTGTTTCAGGGCATGAGCTACTTGGAGGACCGTCGCTTGGTG5180.1719308028305519No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGCGCCGGGC4920.1633010714143466No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC4870.16164150768046096No Hit
CTGGTGTTGTTGGGCAGTCTATCTCCCCTTTCTTGCGGAGATAGACCAAG4160.13807570265928493No Hit
ATACGGCCAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTA4120.13674805167217643No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTCCATCCTCTCAGGG4100.13608422617862218No Hit
TTTCGCTTCCCACAGGTCTCTACGGAAGGCAGTCTGGCTGAGACCAAGTC3920.13010979673663386No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3820.1267906692688626No Hit
GGTCTTCTCTGTTTCAGGGCATGTCGTCAGGGAGGTGGCTTTAGGTCAGC3810.12645875652208546No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG3690.12247580356075993No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG3570.11849285059943442No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA3430.11384607214455462No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA3390.11251842115744613No Hit
ATACGGCCAGGCATTGAAGTACCATGTCGTACGACAGGGGCCAGGAGGAG3230.1072078172090121No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3210.10654399171545785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)