FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301284
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC172795.7351203515619815No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA153305.08822240809336No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA152785.07096294526095No Hit
GTTCAAATGAGTAGACACAGCTTTAGAGAGAGAGAGAGACAGAGATTTCA124774.141275341538217No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA95033.15416683262304No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT92213.06056743803189No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG86862.8829941185061267No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC86182.8604240517252824No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC80922.6858379469205134No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTCTAGCATCTTGTTCTGTTT76852.5507494589822226No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA61632.045578258387435No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA57971.9240981930670067No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA54401.805605342467572No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC48311.6034704796803017No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG44621.4809946761195416No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA40561.346238100928028No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGTAAATGCTATC39111.2981107526453446No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG38461.2765364241048314No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT32071.064444178914247No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT32041.0634484406739155No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT30741.020299783592889No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGATAGAAAGGTAAAGAGGAGCA29770.9881042471555077No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC28460.9446236773277041No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT28180.9353301204179445No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT27270.905126060461226No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT27110.8998154565127919No Hit
GTTCAAATGAGTAGACACAGCTTTAGAGAGAGAGAGACAGAGATTTCATA25500.8463775042816745No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT25190.8360882091315835No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT22980.7627354920938384No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA22300.7401654253129937No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT19620.6512128091767236No Hit
TCATTACACCAGTTCGTCCCTACGGTGGTAAAAAGATAACCTCAGAATAA19580.6498851581896151No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT18080.6000982461730461No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG17830.5918004275036179No Hit
TCCGTCTACTCCCACGTTCTACGGTAGCAGAGAGATGGCAGAAGCTGCTG17600.584166434327744No Hit
CCATGGGACTGACTTTCTGCTACCACCAAGCAATGGATGATTTGATGTAG17180.5702260989631046No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC17080.5669069714953333No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT17070.5665750587485562No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG16400.5443369047144887No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14850.4928904289640339No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT13890.46102680527342965No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCACTGGGT13510.4484141208958989No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG12000.39829529613255266No Hit
CCACCAGTCACTCACACTTGTCGGTAGCAGTGGGATCATGAATGAACATG11190.3714103636436054No Hit
GGCCTCAGTACAAACTCATTAGCGTCTGGACCTAATTACGAGTGACACTG11120.3690869744161655No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT11020.3657678469483942No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA10860.3604572429999602No Hit
CCTGGTCCTCTGACTGCTCTGGCCGTATTGTAGAACCATGTCGTCAGTGT10800.3584657665192974No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT10560.3504998605966464No Hit
ATGAGGTACTCGTCGGCATCGAGACTTGGCTATAGGAGCAGGGGTGTAGA10480.3478445586224293No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCATAGCTCC10360.3438616056611038No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTGCCCCCTGCTTGCCTCTGACCCCT9460.31398945845116233No Hit
ATGAGGTACTCGTCGGCATCCACTGAGACTTGGCTATAGGAGCAGGGGTG9280.3080150290091741No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT9120.30270442506074No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7560.2509260365635082No Hit
ATGAGGTACTCGTCGGCATACACTGAGACTTGGCTATAGGAGCAGGGGTG7490.2486026473360683No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7050.23399848647787472No Hit
GCTGACCTAAAGCCACCTCCCTTCTACCATGCCCTGAAACAGAGAAGACC6900.2290197952762178No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6860.22769214428910925No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA6670.22138580210034386No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC6070.20147103729371624No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGTGAATGCTATC6050.20080721180016198No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT5910.1961604333452822No Hit
GGCCTCAGTACAAACTCATTAGCTACGGTAGCAGCTGGACCTAATTACGA5610.18620305094196837No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA5460.18122435974031148No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5450.18089244699353435No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC5280.1752499302983232No Hit
ATCTCCGCAAGAAAGGGGAGATAGACTGCCCAACAACACCAGTGTAGAAC4140.13741187716573067No Hit
CAGCCAGACTGCCTTCCGTAGAGACCTGTGGGAAGCGAAATGTAGAACCA3840.12745449476241685No Hit
GCTGACCTAAAGCCACCTCCCTGACGACATGCCCTGAAACAGAGAAGACC3760.12479919278819984No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG3750.12446728004142271No Hit
GTTCAAATGAGTAGACACAGCTTTAGAGAGAGAGAGAGAGACAGAGATTT3730.12380345454786847No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT3380.112186508410669No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT3340.1108588574235605No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG3340.1108588574235605No Hit
CCTCCTGGCCCCTGTCGTACGACATGGTACTTCAATGCCTGGCCGTATTG3240.10753972995578923No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG3130.10388868974124083No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT3110.10322486424768657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)