FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217642
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGTGCTGTCAAT123295.664807344170702No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA65693.0182593433252776No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGTGCTGTCAATCTCG44002.021668611756922No Hit
TGTGGTCTGCCAGCTAAAGGACACTGACGACATGGTTTGTACTTTACTTT39371.8089339373834095No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA36301.6678766046994606No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA32131.4762775567215887No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA26621.223109510112938No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC26021.1955413017707979No Hit
TCCAAATACTCCACACGCAAACCAAGTCTCCTCATGGTGGGGGCAGCAGA19820.9106698155686863No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA19650.9028588232050799No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGTGCAGATAATGACAAGGAATA18350.8431277051304435No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA17320.7958022808097702No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA16960.7792613558044862No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC16730.768693542606666No Hit
GCCTGCTGAAAATGACTGAAAATTACTGGTGCAGGACCATTCTAGACCAA16260.7470984460719898No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC15220.6993135516122807No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG15060.6919620293877101No Hit
AGGACAAAATGTTTCACTTTTGGGTACACTGACGACATGGTTCTACAAGG14330.6584207092381066No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG14020.644177134928001No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTTCTTATTCTGAGGGACGAAC13840.6359066724253591No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA12970.5959327703292563No Hit
TGTCCTGCTTGCTTACCTCGGACCAGGAAAAGAGAAGCAAGAGGCAGACC12670.5821486661581864No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG12070.5545804578160465No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC11560.5311474807252277No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT11550.530688010586192No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG11520.529309600169085No Hit
GCCTGCTGAAAATTACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA10440.47968682515323335No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG10120.4649837807040921No Hit
TCTTAAATGGCTACGACCCACACTGACGACATGGTTCTACATCTTAAATG9890.4544159675062717No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG9890.4544159675062717No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG9850.45257808695012913No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG9760.4484428556988081No Hit
AGATGAGTCATATTTGTGGGTTTTCACTGACGACATGGTTCTACATGACC9080.41719888624438295No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG9010.41398259527113335No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC8960.41168524457595496No Hit
TGACAGTTTGACAGTTAAAGGCATACACTGACGACATGGTTCTACATGAC8820.4052526626294557No Hit
GGATGCAGAGCTTCTTCCCATACACTGACGACATGGTTCTACTGGGTGCG8620.3960632598487424No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT7880.3620624695601033No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA7860.36114352928203197No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA7710.35425147719649697No Hit
AGAAAACGGCATTTTGAGTGTACACTGAGGCATAACTGCACCCTTAGACC7610.34965677580614035No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT7590.34873783552806903No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA7330.3367916119131418No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGTACACTGACGACATGGTT7220.33173744038374947No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC7190.33035902996664246No Hit
AGGACAAAATGTTTCACTTTTGGGTGCAGATAATGACAAGGAATATCTAG7140.3280616792714641No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC7110.32668326885435717No Hit
TCACAATTGCCAGTTAACGTCTCTGCTACCGTAGTTTCTGCTTTGCTGTG7030.3230075077420719No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCAGGGACGAACTGGTGTAATG6510.2991150605122173No Hit
AGAAAACGGCATTTTGAGTGTTCAAGGTGAGGCATAACTGCACCCTTAGA6360.29222300842668236No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGT6250.28716883689729No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT5790.2660332105016495No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC5600.25730327785997187No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG5510.2531680466086509No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG5450.2504112257744369No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC5360.24627599452311597No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT5320.2444381139669733No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGTGCTGTCAAT5230.2403028827156523No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT5020.2306540097959034No Hit
TACCCTCCATGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCTACCGT4930.22651877854458238No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACATGAGCAAG4710.21641043548579778No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGACATGGTTCTACAAGAAAAC4670.21457255492965513No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGAATCAGCCGTTACCTGTGTGTG4560.20951838340026283No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA4550.20905891326122714No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT4470.20538315214894184No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC4460.20492368200990618No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT4440.20400474173183483No Hit
TGACAGTTTGACAGTTAAAGGCATTACGGCAGCCGTTACCTGTGTGTGAG4420.20308580145376354No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4380.20124792089762084No Hit
GACCCAAAACCCAAAATGGCCAATCTGAGACGTAGGAGACAGAAGCAGGG4210.1934369285340146No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT4140.1902206375607649No Hit
ACTTCTACCGTGCCCTGACCACTTTGTACAAGAAAGCTGTGTCTACTCAT4090.1879232868655866No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA4060.1865448764484796No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCATATATCACTTTTAAA4020.18470699589233697No Hit
CTGACGACATGGTTCTACACTGACGACATGGTTCTACACTGACGACATGG4020.18470699589233697No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTCTCTGCTACCGTAGCTTGCAAA3940.18103123478005165No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACGTGCTGTCAATCTCG3800.17459865283355236No Hit
TCACAATTGCCAGTTAACGTCTCACTGACGACATGGTTTCTGCTTTGCTG3770.17322024241644535No Hit
ACTTCTACCGTGCCCTGCGGTAGCAGAAGCTGTGTCTACTCATTTGAACA3750.17230130213837402No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT3730.1713823618603027No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC3630.16678766046994606No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCACACTGACGACATGGTT3600.16540925005283907No Hit
TCACAATTGCCAGTTAACGTCTACACTGACGACATGTTTCTGCTTTGCTG3600.16540925005283907No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA3570.16403083963573206No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTAGATGCCGACGAGTACCTCATA3480.1598956083844111No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTACTGGGTGCGGA3470.15943613824537542No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA3350.15392249657694745No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTACACTGACGACATGGTTCTAC3330.15300355629887613No Hit
TGTGCTGTGACTGCTTGTAGGGTAGCACAGAGTTGAAAGTCAGGGCAAGA3310.1520846160208048No Hit
CTGGTGTTGTTGGGCAGTGTCTATCTCCCCTTTCTTGCGGAGATAGACCA3280.15070620560369782No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA3220.14794938476948383No Hit
GCCTGCTGAAAATGACTGAAAATACTGGTGCAGGACCATTCTAGACCAAG3200.1470304444914125No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACCGCG3070.14105733268394888No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG3030.13921945212780623No Hit
TGACCACTGACTCTGATCCAGAAGACCAAGTCTCTGCTACCGTACGTGCT3030.13921945212780623No Hit
ACACTGACGACATGGTTCTACAGCAACAGAGGAGGGGGAGACCAAGTCTC3030.13921945212780623No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC2990.13738157157166356No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGAACCAGCCGTTACCTGTGTGTG2980.1369221014326279No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCGCTAATGAGTTTGTACT2980.1369221014326279No Hit
GACCCAAAACCCAAAATGGCGTACGGTAGGAGACAGAAGCAGGGAAGACC2960.13600316115455657No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT2930.1346247507374496No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC2890.13278687018130692No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG2880.13232740004227125No Hit
GGATGCAGAGCTTCTTCCCAATTGCATGTCGACAGGCTGGGTGCGGAAGA2840.13048951948612858No Hit
CCCCTGCTCCTATAGCCACACTAACGACATGGTTCTACACCCCTGCTCCG2800.12865163892998593No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTAGCAGGGACGAACTGGTGTA2800.12865163892998593No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTACGTGCTG2720.12497587781770063No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA2710.12451640767866497No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG2660.12221905698348665No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGGAAGGCAGTCTGGCTGAGACCA2630.12084064656637966No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCG2610.11992170628830834No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACGT2600.11946223614927266No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGTAGAACCATGACCATGC2520.11578647503698734No Hit
ACTTCTACCGTGCCCTGCGGTAGCAAAGCTGTGTCTACTCATTTGAACAG2480.1139485944808447No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTACGT2480.1139485944808447No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTGAGGGACGAACTGGTGTAAT2440.11211071392470202No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGGCATTTTGAGGCATAACTGC2430.11165124378566638No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGAAGCTGTGTCTACTCAT2420.1111917736466307No Hit
TCAAATCATCCATTGCTTGGGTAGCAGAAAGTCAGTCCCATGGAGACCAA2390.1098133632295237No Hit
TGACCAATGGCTAAGTGAAGATGAGTCATATTTGTGTTTTTCCTTTCCTC2350.10797548267338106No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACTCAAGAG2340.10751601253434538No Hit
TGTGCTGTGACTGCTTGTAGACTGACGACATGGTTCTACAGCAACAGAGT2320.10659707225627406No Hit
GCCTGCTGAAAATGACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA2280.10475919170013141No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCGTGCTAAAATGGAGATTCTC2220.10200237086591742No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACGTGCTGTCAAT2220.10200237086591742No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCAGTGTAACAATAATGA2190.10062396044881042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)