FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217642
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG128955.924867442864888No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA70843.254886464928644No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC45072.070831916633738Illumina Single End PCR Primer 1 (100% over 21bp)
TCTCCCAATGAAAGTAAAGTACAAACCATGTCGTCAGTGTCCTTTAGCTG37891.740932356806131No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA36201.663281903309104No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT30831.4165464386469524No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA25301.1624594517602302No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA25201.1578647503698736No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT23831.0949173413219875No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT20540.943751665579254No Hit
GCTGCCCCCACCATGAGGAGACTTGGTTTGCGTGTGGAGTATTTGGATGT20190.9276702107130058No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT18670.8578307495795847No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG17890.8219920787348031No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT17590.8082079745637331No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGTCATTTTCAGCAGGCTGTAGAA16810.7723693037189513No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA16510.7585851995478814No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA16120.7406658641254905No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG15240.7002324918903521No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAAGTGGTAAAAAGATAACCT13650.6271767397836815No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT13200.6065005835270766No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC13090.6014464119976842No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG13020.5982301210244346No Hit
GCCTCTTGCTTCTCTTTTCCTGGTCCGAGGTAAGCAAGCAGGACATGTAG12840.5899596585217927No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC12410.5702024425432591No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT12330.5665266814309738No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGCAGGCTGTAGAACCATGTCGTC11990.5509046967037612No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT10840.49806563071465987No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCAAAAGTGAAACATTTTGTCC10590.48657887723876825No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC10430.4792273550141976No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC10210.469119011955413No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG9850.45257808695012913No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA9470.4351182216667739No Hit
AAGGGTGCAGTTATGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTG9340.42914510985931026No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG9210.4231719980518466No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA9160.42087464735666824No Hit
TCTGGATCAGAGTCAGTGGTCATGTAGAACCATGTCGTCAGTGAAAACCC8870.40755001332463403No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTATGGGAAGAAGCT8710.40019849110006345No Hit
AAGGGTGCAGTTATGCCTCAGTGTACACTCAAAATGCCGTTTTCTTGTAG8590.39468484943163545No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCTACGGTAGCAGAGACTATCAA8380.3850359765118865No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT8260.37952233484345854No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG8000.3675761112285313No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT7850.3606840591429963No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA7630.3505757160842117No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA7470.34322419385964104No Hit
CCACACAGCAAAGCAGAAACTACGGTAGCAGAGACGTTAACTGGCAATTG7280.33449426121796344No Hit
AAGGGTGCAGTTATGCCTCACCTTGAACACTCAAAATGCCGTTTTCTTGT7270.33403479107892775No Hit
CACACACAGGTAACGGCTGATTCTACATGCCTTTAACTGTCAAACTGTCA7060.32438591815917883No Hit
TCATTACACCAGTTCGTCCCTGGTGGTAAAAAGATAACCTCAGAATAAGA6870.31565598551750124No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC6760.31060181398810893No Hit
CACACACAGGTAACGGCTGCCGTAATGCCTTTAACTGTCAAACTGTCATG6600.3032502917635383No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6470.29727717995607467No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG6450.2963582396780033No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA6360.29222300842668236No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC6000.27568208342139844No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT5760.2646548000845425No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG5670.2605195688332215No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG5630.2586816882770789No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT5510.2531680466086509No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5490.25224910633057956No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC5430.2494922854963656No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC5310.2439786438279376No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC5270.24214076327179496No Hit
CACACACAGGTAACGGCTGGTTCTACATGCCTTTAACTGTCAAACTGTCA5180.23800553202047398No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG4700.2159509653467621No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA4650.21365361465158378No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT4500.20676156256604883No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT4470.20538315214894184No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG4100.18838275700462226No Hit
TCCCTGCTTCTGTCTCCTACGTCTCAGATTGGCCATTTTGGGTTTTGGGT4080.18746381672655094No Hit
GTTCAAATGAGTAGACACAGCTTTCTTGTACAAAGTGGTCAGGGCACGGT4070.18700434658751527No Hit
CTCGATAATCTGGATGACTCATTATTGTTTAAAAGTGATATATGGCACAA4050.18608540630944395No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC4010.1842475257533013Illumina Single End PCR Primer 1 (95% over 22bp)
GTTCAAATGAGTAGACACAGCTTCTGCTACCGCAGGGCACGGTAGAAGTT3860.17735547366776633No Hit
CCACACAGCAAAGCAGAAACCATGTCGTCAGTGAGACGTTAACTGGCAAT3850.17689600352873067No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA3730.1713823618603027No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA3640.16724713060898172No Hit
ATCTCCGCAAGAAAGGGGAGATAGACACTGCCCAACAACACCAGTGTAGA3560.16357136949669643No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA3560.16357136949669643No Hit
CCACACAGCAAAGCAGAAACATGTCGTCAGTGTAGACGTTAACTGGCAAT3500.16081454866248243No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTTGTAGAACCATG3480.1598956083844111No Hit
ATGAGGTACTCGTCGGCATCTAGCAGAGACTTGGCTATAGGAGCAGGGGT3450.1585171979673041No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTGGGAAGAAGCTCT3440.15805772782826844No Hit
TGCCCTGACTTTCAACTCTGTGCTACCCTACAAGCAGTCACAGCACATGT3440.15805772782826844No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG3370.15484143685501878No Hit
AGAATGGTCCTGCACCAGTATTTTCAGTCATTTTCAGCAGGCTGTAGAAC3330.15300355629887613No Hit
CACACACAGGTAACGGCTGTCAAACTGTCATGTAGAACCATGTCGTCAGT3260.1497872653256265No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA3140.1442736236571985No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTCTTGTAGAACCATGTCGT3080.14151680282298454No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG3020.13875998198877054No Hit
TCCCTGCTTCTGTCTCCTACCGTACGCCATTTTGGGTTTTGGGTCTGTAG3020.13875998198877054No Hit
TCTGGATCAGAGTCAGTGGTCATGTAGAACCATGTCGTCAGTGTAGATCT3010.1383005118497349No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT2960.13600316115455657No Hit
GGCCTCAGTACAAACTCATTAGCGAGACTTGGTCTGGACCTAATTACGAG2940.13508422087648522No Hit
TCATTACACCAGTTCGTCCCTGCTAGTGGTAAAAAGATAACCTCAGAATA2900.13324634032034258No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG2860.13140845976419993No Hit
TTCTCTTCCGCACCCAGCCTGTCGACATGCAATTGGGAAGAAGCTCTGCA2810.12911110906902162No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT2770.12727322851287895No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAATGTAGAACCATGTCGTCA2760.1268137583738433No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC2750.12635428823480763No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAACCATGTCG2670.12267852712252231No Hit
CAGCCAGACTGCCTTCCGGTAGCAGAGACCTGTGGGAAGCGAAATGTAGA2640.12130011670541531No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG2600.11946223614927266No Hit
ATGAGGTACTCGTCGGCATCGGAGCAGGGGTGTAGAACCATGTCGTTAGT2580.11854329587120134No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC2580.11854329587120134No Hit
AGAATGGTCCTGCACCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC2570.11808382573216566No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA2570.11808382573216566No Hit
GCCTCAAAGACAATGGCTTTGCCTGATACAGATGTAGAACCATGTCGTCA2540.11670541531505867No Hit
TCATTACACCAGTTCGTCCCTCAGTGGTAAAAAGATAACCTCAGAATAAG2520.11578647503698734No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA2490.11440806461988035No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTCAGTGTACACTCAAAATGCCG2480.1139485944808447No Hit
GTTCAAATGAGTAGACACAGCTTGCATGGTCATGGTTCTACAGGTCTTCT2470.11348912434180902No Hit
GTTCAAATGAGTAGACACAGCTTTGCTACCGCAGGGCACGGTAGAAGTTG2460.11302965420277337No Hit
CCATGGGACTGACTTTCTGCTACCCAAGCAATGGATGATTTGATGTAGAA2450.1125701840637377No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTCTACAA2450.1125701840637377No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAAGGCAATCTGA2420.1111917736466307No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAAAACC2380.10935389309048806No Hit
GTTCAAATGAGTAGACACAGCTTCAGGTCTTCTCTGTTTCAGGGCACGGT2320.10659707225627406No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGCAGCACAACTAAAAGAA2300.10567813197820274No Hit
TCCAGGAAGAGGAAAGGAAAAACACAAATATGACTCATCTTCACTTAGCC2280.10475919170013141No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2270.10429972156109574No Hit
TTCATGGTGTTTTATCCCTCTTGAGTAGCAGAGACTTGGTCTTTCATGGA2250.10338078128302441No Hit
ACTAGATATTCCTTGTCATTATCTGCACATGGGTCTACCCAAAAGTGAAA2230.10246184100495309No Hit
CTCGATAATCTGGATGACTCATTATTGTTACACTGGCACAACTAAAAGAA2220.10200237086591742No Hit
ATGAGGTACTCGTCGGCATCGTCAGTGTGGCTATAGGAGCAGGGGTGTAG2220.10200237086591742No Hit
AACAGAGAATCTCCATTTTAGCACGCAGAGACTTTGTCATATTTTTCCCC2190.10062396044881042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)