FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479874
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA299756.246431354897327No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT251145.233457115826238No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA210634.389277185261131No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC181453.7812008985692076No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC140462.9270183423148577No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT134932.8117797588533655No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC106852.2266261560326255No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG100522.0947165297557273No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA99802.0797125912218624No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG98062.043453073098355No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC87441.8221449797238443No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT80951.6869011448838653No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG79431.6552261635345944No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG79241.6512667908659358No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA65121.3570228851740247No Hit
TGTGGTCTGCCAGCTAAAGGACACTGACGACATGTGGTTTGTACTTTACT64001.3336834252324568No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT59411.2380333170790667No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC59231.2342823324456003No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA58521.2194867819469277No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA57381.1957305459349745No Hit
GTAGAGGAGCCGTCAAATCCATGCTCAAGAGGGATAAAACACCATGAAAG56331.1738498022397545No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC55941.1657226688672444No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC52901.1023727061687025No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG52371.0913281403034962No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA44540.9281603087477129No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT43180.8998195359615232No Hit
TCCCTGCCAGCGAGATCTCCTCCATCTTGGAGAAAGGAGAACGCCTCCCT39920.8318850364887449No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA38650.8054197560192884No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC34280.7143541846401347No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT33900.706435439302817No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG33450.6970579777191512No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCGTGTGGGGGTTGTTGACTTGTAG31530.6570474749621776No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT30150.6282899261056027No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG29610.6170369722052039No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC29120.6068259584807678No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG28860.6014078695657611No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT28780.5997407652842205No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28660.5972401088619096No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA28660.5972401088619096No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGT27970.5828613344336222No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG27060.5638980232310982No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT26340.548894084697233No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC25100.5230539683333542No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC24860.5180526554887325No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT24580.5122177905033405No Hit
AGAAAACGGCATTTTGAGTGTGGGTGAGGCATAACTGCACCCTTAGACCA24220.5047158212364079No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC23940.49888095625101586No Hit
CAGAGGGGAAAAATATGACAAAGTCGTGCTAAAATGGAGATTCTCTGTTA22950.4782505407669513No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT22040.45928722956442736No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT20370.42448642768726796No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGAGTGCATCTAT19780.4121915336109062No Hit
GGTCTTCTCTGTTTCAGGGCATAAATCACTTGAACTGGGAGGTGGCTTTA19420.40468956434397363No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA18530.38614302921183474No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC18410.3836423727895239No Hit
GTGTCACTCGTAATTAGGTCCAGTGTGCTAATGAGTTTGTACTGAGGCCA16690.34779963073640163No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC16170.33696345290638796No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16050.33446279648407706No Hit
GGAATCCTATGGCTTTCCAACCATGGTTCTACAGCAACAGAGGAGGGGGA15880.33092019988580335No Hit
ACTTCTACCGTGCCCTGAGAAGCTGTGTCTACTCATTTGAACAGACCAAG14480.30174587495884336No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA13160.2742386543134239No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG12590.2623605363074474No Hit
GGAAACCGTAGCTGCCCTGCATTGACGACATGGTTCATCTGGACCTGGGT12130.2527746866885891No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG11930.24860692598473766No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG11750.24485594135127137No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC11420.2379791361899165No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCAGGTGCCGGGC10470.21818227284662223No Hit
GGAATCCTATGGCTTTCCAACCCCCCCAAACGCAGCAACAGAGGAGGGGG10330.21526484035392623No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAATTG9830.20484543859429766No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC9570.1994273496792908No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC9050.1885911718492771No Hit
CCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA8930.18609051542696625No Hit
GGAAACCGTAGCTGCCCTGGGTCTTCATCTGGACCTGGGTCTTAGACCAA8630.17983887437118912No Hit
TCCCTGCCAGCGAGATGTACACTGAGGCCTTTGCATCCCTAGACCAAGTC8630.17983887437118912No Hit
GGTCTTCTCTGTTTCAGGGCATGGAGGTGGCTTTAGGTCAGCAGACCAAG8590.17900532223041882No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT8570.17858854616003367No Hit
ACTTCTACCGTGCCCTGACCCCAAGCTGTGTCTACTCATTTGAACAGACC8390.17483756152656738No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG7490.15608263835923597No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC7450.1552490862184657No Hit
GGGGTCAGAGGCAAGCAGGGAAGACGGAGATAACACAGGCCCAAGAGACC6630.13816126733267484No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA6310.13149285020651255No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA5910.12315732879880968No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC5770.12023989630611369No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC5630.11732246381341768No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAG5560.1158637475670697No Hit
GGTCTTCTCTGTTTCAGGGCATCTGCTACCGTAGGAGGTGGCTTTAGGTC5420.1129463150743737No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG5400.11252953900398854No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCGCTAATGAGTTTGTACTGA5380.1121127629336034No Hit
GGAAACCGTAGCTGCCCTGCAGTGTGACCTGGACTGTATCAGCTTCATCT5060.10544434580744112No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5030.10481918170186341No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA4970.10356885349070796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)