FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479874
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC217604.534523645790353No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA205204.276122482151565No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA197824.122332112179447No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA169013.5219661827896487No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG141602.950774578326811No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC134892.8109462067125954No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC110532.3033129529834917No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG103372.1541071197856105No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA99602.075544830518011No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA99432.0720022339197373No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG95931.999066421602337No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA83201.733788452802194No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC81501.6983624868194567No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA73111.5235249252928895No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG71821.4966428687530478No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT67421.4049521332683164No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA66181.3791120169044375No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT64051.3347253654084197No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT62191.2959651908626013No Hit
TCTCCCAATGAAAGTAAAGTACAAACCACATGTCGTCAGTGTCCTTTAGC61401.2795025360823882No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC59931.2488694949090802No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC57731.2030241271667146No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA56971.1871866364920791No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA55781.1623884603041632No Hit
TTCATGGTGTTTTATCCCTCTTGAGCATGGATTTGACGGCTCCTCTACTG55631.1592626397762746No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT54041.1261289421806557No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA44030.9175325189528918No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA41700.8689781067530227No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT38500.8022939354913999No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA33890.7062270512676244No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT33130.690389560592989No Hit
ACAAGTCAACAACCCCCACACGACTTCCATTTTCTACTTTTTCTGAGGTG31380.653921654434289No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT29100.6064091824103828No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT28570.5953646165451765No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG28280.5893213635245919No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG27590.5749425890963045No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC26400.5501444129083884No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT25610.5336817581281753No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG25020.5213868640518136No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT24740.5155519990664217No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCCATC24580.5122177905033405No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT24350.5074248656939113No Hit
AAGGGTGCAGTTATGCCTCACCCACACTCAAAATGCCGTTTTCTTGTAGA24260.5055493733771782No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG23830.4965886878638976No Hit
AACAGAGAATCTCCATTTTAGCACGACTTTGTCATATTTTTCCCCTCTGT22860.4763750484502182No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC22270.46408015437385647No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC21970.45782851331807933No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT21810.45449430475499825No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC21360.4451168431713325No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG20030.4174012344907205No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCACAGAAAATCTTT19800.41260830968129136No Hit
GCTGACCTAAAGCCACCTCCCAGTTCAAGTGATTTATGCCCTGAAACAGA19460.4055231164847439No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT18540.3863514172470274No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC18320.3817668804727908No Hit
GGCCTCAGTACAAACTCATTAGCACACTGGACCTAATTACGAGTGACACT16610.3461325264548611No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG16150.33654667683600276No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGTTGGAAAGCCATAGGATTCCT15810.3294614836394553No Hit
GTTCAAATGAGTAGACACAGCTTCTCAGGGCACGGTAGAAGTTGTAGAAC14450.30112071085326564No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC12500.2604850439907142No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA12470.2598598798851365No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG11950.2490237020551228No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAATGCAGGGCAGCTACGGTT11880.24756498580877478No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC11690.24360561314011592No Hit
CCCCCTCCTCTGTTGCTGCGTTTGGGGGGGTTGGAAAGCCATAGGATTCC10310.2148480642835411No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT10160.21172224375565252No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA10050.20942997536853425No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT9960.2075544830518011No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT9460.19713508129217253No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG9000.18754923167331425No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG8690.18108920258234454No Hit
AAGACCCAGGTCCAGATGAAGACCCAGGGCAGCTACGGTTTCCTGTAGAA8610.17942209830080397No Hit
AGGGATGCAAAGGCCTCAGTGTACATCTCGCTGGCAGGGATGTAGAACCA8510.17733821794887825No Hit
GCTGACCTAAAGCCACCTCCATGCCCTGAAACAGAGAAGACCTGTAGAAC8500.17712982991368567No Hit
GTTCAAATGAGTAGACACAGCTTGGGGTCAGGGCACGGTAGAAGTTGTAG8380.17462917349137483No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7620.1587916828167394No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7610.1585832947815468No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA7460.15545747425365825No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG7400.15420714604250282No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7010.14608001266999254No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT6930.14441290838845197No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC6730.14024514768460056No Hit
CTTGGGCCTGTGTTATCTCCGTCTTCCCTGCTTGCCTCTGACCCCTGTAG6520.13586899894555654No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA6250.1302425219953571No Hit
CCAACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCC5940.12378249290438739No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5830.12149022451726911No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT5720.11919795613015083No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG5630.11732246381341768No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA5480.11419664328552913No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAGAACAAGATTTACCTCTA5400.11252953900398854No Hit
GCTGACCTAAAGCCACCTCCTACGGTAGCAGATGCCCTGAAACAGAGAAG5400.11252953900398854No Hit
GGCCTCAGTACAAACTCATTAGCGACTTGGTCTGGACCTAATTACGAGTG5370.11190437489841083No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT5290.11023727061687026No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT4950.10315207742032284No Hit
AAGACCCAGGTCCAGATGAAGCTGATACAGTCCAGGTCACACTGCAGGGC4940.10294368938513025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)