FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG133.91566265060241No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT123.614457831325301No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA123.614457831325301No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA113.313253012048193No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA103.0120481927710845No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG103.0120481927710845No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA103.0120481927710845No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC92.710843373493976No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT92.710843373493976No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT82.4096385542168677No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC82.4096385542168677No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA72.108433734939759No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG72.108433734939759No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT72.108433734939759No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC61.8072289156626504No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG61.8072289156626504No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC51.5060240963855422No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT51.5060240963855422No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG51.5060240963855422No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT51.5060240963855422No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC51.5060240963855422No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT51.5060240963855422No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG51.5060240963855422No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA41.2048192771084338No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC41.2048192771084338No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG41.2048192771084338No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG41.2048192771084338No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA41.2048192771084338No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG41.2048192771084338No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC41.2048192771084338No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC30.9036144578313252No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT30.9036144578313252No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA30.9036144578313252No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG30.9036144578313252No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA30.9036144578313252No Hit
ACTTCTACGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGATG30.9036144578313252No Hit
GCCTGCTGAAGATGACTGAATATAAACTTGTGATAGTTGGAGCTGGTGGC30.9036144578313252No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC30.9036144578313252No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA20.6024096385542169No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT20.6024096385542169No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGTAACTG20.6024096385542169No Hit
GCTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT20.6024096385542169No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACTA20.6024096385542169No Hit
AGCTGTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTC20.6024096385542169No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG20.6024096385542169No Hit
GTAGGGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC20.6024096385542169No Hit
GTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCAC20.6024096385542169No Hit
AGCCACATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTCACAA20.6024096385542169No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAAGAGAACGCCTCCCT20.6024096385542169No Hit
TTCTTATTCTGAGGTTATCATTTTACCACAGTCGCACAATATCCTTTTGA20.6024096385542169No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA20.6024096385542169No Hit
CAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGTTAAAGAATCATC20.6024096385542169No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC20.6024096385542169No Hit
TGTCCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA20.6024096385542169No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG20.6024096385542169No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT20.6024096385542169No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCCCCCAG20.6024096385542169No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACAGGCCATTCT20.6024096385542169No Hit
GCAGCCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGACATG10.30120481927710846No Hit
NGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA10.30120481927710846No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAGGCCAGCCCCTCAGGGCAACTG10.30120481927710846No Hit
GCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG10.30120481927710846No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG10.30120481927710846No Hit
TTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCG10.30120481927710846No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAGGGAGAACGCCTCCCT10.30120481927710846No Hit
TCTTAAGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTATAAT10.30120481927710846No Hit
CTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAG10.30120481927710846No Hit
ATACGGCCAGGCATTGAAGTCTTATGGAAGCCAGCCCCTCAGGGCAACTG10.30120481927710846No Hit
TGTGGTCTGCCAGCTAAGGGTGAAGATATATTCCTCCAATTCAGGACCCA10.30120481927710846No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGTTGTTGAC10.30120481927710846No Hit
AGCGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCTC10.30120481927710846No Hit
AAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAA10.30120481927710846No Hit
GGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCAG10.30120481927710846No Hit
TCCACAAACAGAACAAGATGCTACTACCGTAAATCCTGTTTGAAGGAAAA10.30120481927710846No Hit
GGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTAGACCAAGT10.30120481927710846No Hit
TCCACAAACAGAAAAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG10.30120481927710846No Hit
ACTCCTACCGTGCCCTGATGGATGAAAAAGACATGGACGAGGTGGTGGAT10.30120481927710846No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGCGCTAGTAT10.30120481927710846No Hit
TCATAATGCTTGCTCTGAAAGGAAAATGAGATCTACTGTTTTCCTTTACT10.30120481927710846No Hit
TCCAAATACTCCACACGCAAATTTCCTTTCACTCGGATAAGATGCTGAGG10.30120481927710846No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA10.30120481927710846No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTTAGGGCAACTG10.30120481927710846No Hit
AATCTGTCTTTTGGTTTTTCTTGTAGTATTAATGTAATTTCAAATGTTAG10.30120481927710846No Hit
CAGTCAGAGGCGCTATGGTATTATTATAGCTACCTGTTAAAGAATCATCT10.30120481927710846No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAAGCTG10.30120481927710846No Hit
CTTCTACCGTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGG10.30120481927710846No Hit
GCGTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC10.30120481927710846No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAAGTTCCCAG10.30120481927710846No Hit
TATATCACTTTTAAATTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA10.30120481927710846No Hit
TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGGTCTCGTGTTCCT10.30120481927710846No Hit
GGCTTCGGCCCCTTACTTGAGGATAAATTATGTCCAATATTCAAACTGGC10.30120481927710846No Hit
NGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA10.30120481927710846No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTACGTGATCAAGAAATTGATAGC10.30120481927710846No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT10.30120481927710846No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAGGAATCATC10.30120481927710846No Hit
TTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATT10.30120481927710846No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG10.30120481927710846No Hit
AGCTGCTCACCATCGCTATCTGAACAGCGCTCATGGTGGGGGCAGCGCCT10.30120481927710846No Hit
TCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG10.30120481927710846No Hit
CTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAA10.30120481927710846No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGGGGG10.30120481927710846No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCCGTAACACC10.30120481927710846No Hit
TTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAG10.30120481927710846No Hit
GGTCTTCTCTATTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG10.30120481927710846No Hit
GCAACAGAACAAGATGCTAAAAAAGGACAAAGTGTTTCACTTTTGGGTAA10.30120481927710846No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG10.30120481927710846No Hit
GCGTGGACAACCCCCACACACTGACGACATGATTCTACAGCAACAGAGGA10.30120481927710846No Hit
AGAAAACGGCATTTTAAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG10.30120481927710846No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA10.30120481927710846No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10.30120481927710846No Hit
GGAATCCTATGGCTTTCCAACCTGGGAAGGTAGGGGAGTAGGGCCAGGAA10.30120481927710846No Hit
GGTCTTCTCTGTCTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG10.30120481927710846No Hit
GCCTGCTGAAAATGACTGAATATAAACTCGTGGTAGTTGGAGCTGGTGGC10.30120481927710846No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA10.30120481927710846No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGACCAGGAA10.30120481927710846No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTGCGCTAGTATCTCG10.30120481927710846No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG10.30120481927710846No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTTATACCAGGACCA10.30120481927710846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)