FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC164.819277108433735No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA164.819277108433735No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT133.91566265060241No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC123.614457831325301No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA113.313253012048193No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT113.313253012048193No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC103.0120481927710845No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA82.4096385542168677No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG72.108433734939759No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA72.108433734939759No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT72.108433734939759No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT72.108433734939759No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA72.108433734939759No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG72.108433734939759No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT72.108433734939759No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC61.8072289156626504No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG51.5060240963855422No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC51.5060240963855422No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC51.5060240963855422No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT51.5060240963855422No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG51.5060240963855422No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT51.5060240963855422No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA41.2048192771084338No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG41.2048192771084338No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT41.2048192771084338No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT41.2048192771084338No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC41.2048192771084338No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA30.9036144578313252No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA30.9036144578313252No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA30.9036144578313252No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT30.9036144578313252No Hit
TCCAGGAAGAAGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT30.9036144578313252No Hit
GCTGATCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT30.9036144578313252No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC30.9036144578313252No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT30.9036144578313252No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGGGATTTCATA20.6024096385542169No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAGAACCTACCAGGGC20.6024096385542169No Hit
CACACACAGGTAAGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCCC20.6024096385542169No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT20.6024096385542169No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTACAAATGCTATC20.6024096385542169No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC20.6024096385542169No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTTTG20.6024096385542169No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAGTAATACACATAGCGCCTCTG20.6024096385542169No Hit
GCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT20.6024096385542169No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT20.6024096385542169No Hit
TCCAGATGTTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTGA20.6024096385542169No Hit
TCTGGATCAGAGTCAGTGGTGCCGGAATATCTATAATGATCAGGTTCATT20.6024096385542169No Hit
GGTCTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGA20.6024096385542169No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT20.6024096385542169No Hit
GGCTCCTTATTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGAT10.30120481927710846No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC10.30120481927710846No Hit
CTCGATAATCTGGATGTCTCATTATTGTTATGACAGTAAGATACAGTCTA10.30120481927710846No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTTCTCCCCTG10.30120481927710846No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCCA10.30120481927710846No Hit
CCCCCTCCTCTGTTGCTACAGATCCGTGGGCGTGAGCGCTTCGAGATGTT10.30120481927710846No Hit
ACTAGATAGTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC10.30120481927710846No Hit
ACAAGTCAACAACCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGC10.30120481927710846No Hit
TTCATGGTTCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAATTTGTGT10.30120481927710846No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT10.30120481927710846No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAAGGC10.30120481927710846No Hit
GCTGACCTAAAGCCAACTCGTTACTTTTCCTCAGTATGCAAGATATAATT10.30120481927710846No Hit
CACGCACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC10.30120481927710846No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGCGACAGAAAGGTAAAGAGGAGCA10.30120481927710846No Hit
TTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACT10.30120481927710846No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA10.30120481927710846No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTA10.30120481927710846No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCGTT10.30120481927710846No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG10.30120481927710846No Hit
GTTCAAATGAGGCAGCTTGAGAGAGAGAGAGAGACAGAGATTTCATACCA10.30120481927710846No Hit
GTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTGGTT10.30120481927710846No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC10.30120481927710846No Hit
GTTTCCTCTGGTCCTGGTATGAACAATGTATTTACCCAAAAGTGAAACAT10.30120481927710846No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT10.30120481927710846No Hit
CCATGGGACTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCC10.30120481927710846No Hit
ACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGA10.30120481927710846No Hit
AAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGTCA10.30120481927710846No Hit
CCGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC10.30120481927710846No Hit
CACACGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCCCGT10.30120481927710846No Hit
CCAACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCC10.30120481927710846No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACGT10.30120481927710846No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA10.30120481927710846No Hit
TCTAGATATGGCTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT10.30120481927710846No Hit
CTCCAAGATTTGGAGGCATGAAAACATACAATTGGGAGGGTGTCAATCCT10.30120481927710846No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTTCAAAAAGGGTCAGTCT10.30120481927710846No Hit
AGAATGGGCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10.30120481927710846No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT10.30120481927710846No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGTGACG10.30120481927710846No Hit
CCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACGA10.30120481927710846No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGTAGCATCTTGTTCTGTTTG10.30120481927710846No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC10.30120481927710846No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC10.30120481927710846Illumina Single End PCR Primer 1 (100% over 21bp)
CTGCTTGGCCCCACCTCAACGAAATTTCTCAGGCGCGAGATCAACAGCGC10.30120481927710846No Hit
CACACACAGGTAACGGCTGAGGGAATTCAAAATACATGAACTTGTCCTCC10.30120481927710846No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA10.30120481927710846No Hit
AAGGGCGCACGTATGCCACGCCCAACCCGGCAGGAGCCACCCCGGCCGAT10.30120481927710846No Hit
AAGGGTGCATGCCTCAGATCCACTTTTATCACCTTTCCTTGCCTCTTTCC10.30120481927710846No Hit
GTTTCCTTTGGTCCTGGTATGAACAATGTATTTACCCAAAAGTGAAACAC10.30120481927710846No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCCTGTAGAACC10.30120481927710846No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAATCATGTCGTCAGTGTG10.30120481927710846No Hit
CCACCAGTCACTCACAGTTGACCATGATCATGTAGACATCGATGGTACAT10.30120481927710846No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACTTT10.30120481927710846No Hit
TCTCCTAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT10.30120481927710846No Hit
AAGACCCAGGTCCAGGTGAAGCTCCCAGAACGCCAGAGGCTGCTCCCCGC10.30120481927710846No Hit
CTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATTG10.30120481927710846No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGCAGCTAGACCAAAATCAC10.30120481927710846No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGC10.30120481927710846No Hit
GTTCAAATGAGTATACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA10.30120481927710846No Hit
GTTCAAATGAGTACACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA10.30120481927710846No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCGCTTGTAGAACCATG10.30120481927710846No Hit
CGGCTTTTTGACCGCCTCCAAACAATTTAGACATGGCGCCACCAGCAAGA10.30120481927710846No Hit
CTCGATAATCTGGATGACTCTTATTGTTATGACAGTAAGATACAGTCTAT10.30120481927710846No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC10.30120481927710846No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCCCCATCCAC10.30120481927710846No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT10.30120481927710846No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG10.30120481927710846No Hit
TCTAGATGATTCCTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG10.30120481927710846No Hit
ACAGTCGGGAGAGGAGTGGCATTAACACCACCCTTCATGAACTTAATCCA10.30120481927710846No Hit
CCACACAGCAAAGCGGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG10.30120481927710846No Hit
TTGATGGGACCCACTACATCGAGATTTCACTGTAGCTAGACCAAAATCAC10.30120481927710846No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG10.30120481927710846No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG10.30120481927710846No Hit
AGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCTCTG10.30120481927710846No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG10.30120481927710846No Hit
ACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGC10.30120481927710846No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA10.30120481927710846No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10.30120481927710846No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTTTTCTGTTT10.30120481927710846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)