FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260532
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCTACTGTCAT135955.218168977323323No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA71732.7532126571783886No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTCTACTGTCATCTCG51391.9725024181290591No Hit
TGTGGTCTGCCAGCTAAAGGGTCTGTTTCCTCTGGTCCTGGTTTGTACTT39261.5069166167687653No Hit
GCCTGCTGAAAATGACTGACGACATGGTTCTACAGCCTGCTGAAAATTAC36801.4124944344648642No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA35681.3695054734159335No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA30501.1706815285646295No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA27791.0666635960265918No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC26461.0156142047809866No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA22290.855557090875593No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC19620.7530744783750173No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG19490.7480846882532665No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG19380.7438625581502464No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19240.7384889380191301No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA19120.7338829779067446No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG16830.6459859057620562No Hit
AGGACAAAATGTTTCACTTTTGGGTACACTGACGACATGGTTCTACAAGG16070.6168148250502817No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGTGCAGATAATGACAAGGAATA15970.6129765249566271No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA15490.5945526845070855No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG15410.5914820444321619No Hit
GCCTGCTGAAAATTACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA14780.5673007538421384No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG14110.5415841432146531No Hit
TGACAGTTTGACAGTTAAAGGCATACACTGACGACATGGTTCTACATGAC13990.5369781831022676No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA12590.4832419817911044No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG12480.47901985168808436No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT12260.4705755914820444No Hit
TCCAAATACTCCACACGCAACACTGACGACATGGTTCTACATCCTCATGG11520.44217217078900095No Hit
TCTTAAATGGCTACGACCCACACTGACGACATGGTTCTACATCTTAAATG11290.4333440805735955No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG10880.4176070501896121No Hit
TGTTCATTCATGATCCCACTGCTACACACTGACGACATGGTTCTACATGT10730.41184960004913024No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGTACACTGACGACATGGTT10580.40609214990864845No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC10540.40455682987118663No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10130.3888197994872031No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA10070.3865168194310104No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC9960.38229468932799043No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC9540.36617382893464145No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC9190.35273977860685063No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT9070.3481338184944652No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC8820.33853806826032884No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG8710.33431593815730887No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA8590.32970997804492347No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC8540.3277908279980962No Hit
AGGACAAAATGTTTCACTTTTGGGTGCAGATAATGACAAGGAATATCTAG8060.3093669875485545No Hit
TCTGTATCAGGCAAAGTCATAGACACTGACGACATGGTTCTACATCTGTA8000.3070640074923618No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA7840.3009227273425146No Hit
AGATGAGTCATATTTGTGGGTTTTCACTGACGACATGGTTCTACATGACC7520.2886401670428201No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG7470.28672101699599284No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG7120.27328696666820196No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTC7010.269064836565182No Hit
AGAAAACGGCATTTTGAGTGTACACTGAGGCATAACTGCACCCTTAGACC6980.26791334653708565No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT6750.2590852563216802No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGTAGAACAAGCTGTGTCT6640.25486312621866025No Hit
TCCAAATACTCCACACGCAAAGCCTTCCTACTCATGGTGGGGGCAGCAGA6580.2525601461624676No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCACACTGACGACATGGTT6560.25179248614373667No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG6240.23950992584404218No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACAACAATAATGAGTC6210.23835843581594585No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA5980.22953034560054042No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC5880.22569204550688593No Hit
TCATAATGCTTGCTCTGATAGGATACACTGACGACATGGTTCTACATCAT5870.22530821549752045No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGACTTGGTCTAAGGGTGCAGATA5820.22338906545069317No Hit
CTGGTGTTGTTGGGCAGTGTCTATCTCCCCTTTCTTGCGGAGATAGACCA5640.21648012528211508No Hit
TGTGCTGTGACTGCTTGTAGGGTAGCACAGAGTTGAAAGTCAGGGCAAGA5590.2145609752352878No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGACATGGTTCTACAAGAAAAC5550.213025655197826No Hit
TACCCTCCATGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCTACCGT5520.21187416516972962No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCTACTGTCAT5520.21187416516972962No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG5380.2065005450386133No Hit
TCACAATTGCCAGTTAACGTCTACACTGACGACATGGTTTCTGCTTTGCT5350.20534905501051695No Hit
TCCAAATACTCCACACGCAAACTGACGACATGGTTCTACATCCTCATGGT5300.20342990496368968No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGACCAGAGGAAACAGACAGGG5280.2026622449449588No Hit
GACCCAAAACCCAAAATGGTACGGTAGGAGACAGAAGCAGGGAAGACCAA5230.20074309489813152No Hit
ACTTCTACCGTGCCCTGACAACTTTGTATAGAAAAGCTGTGTCTACTCAT5190.1992077748606697No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTACTGGGTGCGGA5100.19575330477638064No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCACCGTAACAATAATGA4880.1873090445703407No Hit
CTGACGACATGGTTCTACACTGACGACATGGTTCTACACTGACGACATGG4880.1873090445703407No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4840.18577372453287888No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTAGTCTACTGTCATCTCG4780.18347074447668615No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG4660.17886478436430076No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCGGTAGCAGGGACGAACTGGT4650.1784809543549353No Hit
GTAGAGGAGCCGTCAAATCCATTGGGGTTTCCCCGCGCAGGTTCGAATCA4640.17809712434556985No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGAAGCTGTGTCTACTCATTTGAA4630.1777132943362044No Hit
GACCCAAAACCCAAAATGGCACGGTAGGAGACAGAAGCAGGGAAGACCAA4590.17617797429874257No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA4570.17541031428001166No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG4510.17310733422381894No Hit
TCAAATCATCCATTGCTTGGGTAGCAGAAAGTCAGTCCCATGGAGACCAA4410.16926903413016442No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC4390.1685013741114335No Hit
ACTTCTACCGTGCCCTGCGGTAGCAGAAGCTGTGTCTACTCATTTGAACA4370.16773371409270263No Hit
AGAAAACGGCATTTTGAGTGTCTGCTACCGTATGAGGCATAACTGCACCC4190.16082477392412448No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGCTACCGTAACAATAA4140.15890562387729723No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT3950.15161285369935362No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTAGCAGGGACGAACTGGTGT3890.14930987364316092No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTACACTGACGACATGGTTCTAC3860.14815838361506456No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA3850.1477745536056991No Hit
GCCTGCTGAAAATGACTGAAAATTACTGGTGCAGGACCATTCTAGACCAA3810.14623923356823731No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGTC3800.14585540355887186No Hit
TCCAAATACTCCACACGCAAGGCCTTCCTACTCATGGTGGGGGCAGCAGA3760.14432008352141004No Hit
ACACTGACGACATGGTTCTACAGCAACAGAGGAGGGGGAGACCAAGTCTC3700.14201710346521734No Hit
TCACAATTGCCAGTTAACGTCTCTGCTTTGCTGTGTGGAGACCAAGTCTC3640.13971412340902462No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT3630.13933029339965916No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAACACTGACGACATGGTTCTA3550.13625965332473552No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC3550.13625965332473552No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGATGCCGACGAGTACCTCA3480.13357284325917737No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTAGGGACGAACTGGTGTAAT3430.1316536932123501No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACGTGCTAAAATGGAGATT3430.1316536932123501No Hit
TGTGCTGTGACTGCTTGTAGCACAGAGTTGAAAGTCAGGGCAAGACCAAG3410.13088603319361922No Hit
GGATGCAGAGCTTCTTCCCAATTGCATGTCGACAGGCTGGGTGCGGAAGA3360.12896688314679194No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA3320.12743156310933013No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTTGGTTCTACATCATAATGCTTG3290.1262800730812338No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG3260.12512858305313743No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACCGCG3230.12397709302504106No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAGAACGTGGGAGTAGACGGAAG3210.12320943300631017No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCACTGACGACATGGTTCTACA3150.12090645295011745No Hit
GGATGCAGAGCTTCTTCCCACAGGTCTCTGCTACCGTACTGGGTGCGGAA3150.12090645295011745No Hit
TGACCACTGACTCTGATCCAGAAGACCAAGTCTCTGCTACCGTAGTCTAC3110.11937113291265564No Hit
CCCCTGCTCCTATAGCCATGCGACTGTGACAATCTTAGATGCCGACGAGT3080.11821964288455929No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA3050.11706815285646294No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT3030.11630049283773201No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGCCTTGGTTTTAGGGACGAAC3010.11553283281900112No Hit
TGACAGTTTGACAGTTAAAGGCATACCAAGTCTCTGCTACCGCAGCCGTT3000.11514900280963566No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGTC2980.11438134279090477No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA2970.11399751278153931No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC2950.11322985276280842No Hit
AGAAAACGGCATTTTGAGTGTGAGGCATAACTGCACCCTTAGACCAAGTC2940.11284602275344296No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT2940.11284602275344296No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT2870.11015921268788478No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA2850.10939155266915389No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGTCTACT2830.10862389265042299No Hit
TACCCTCCATGAGGCACACTGACGACGACAAGGACTACAAGGACGACGAC2820.10824006264105754No Hit
TCCAAATACTCCACACGCAAAGCCCTCATGGTGGGGGCAGCAGACCAAGT2780.10670474260359572No Hit
TCCAAATACTCCACACGCAAAGCCTCATGGTGGGGGCAGCAGACCAAGTC2770.10632091259423027No Hit
TGTTCATTCATGATCCCACTGCTACACACTGACGACATGGTCTACATGTT2650.10171495248184484No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATTGATGTTTAT2640.1013311224724794No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT2640.1013311224724794No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT2610.10017963244438305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)