FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260532
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG143715.516021064590914No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA78503.0130655735188No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC52882.0296930895245113Illumina Single End PCR Primer 1 (100% over 21bp)
AGAATGGTCCTGCACCAGTAATTTTCAGCAGGCTGTAGAACCATGTCGTC52172.0024411588595643No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT49621.904564506471374No Hit
TCTCCCAATGAAAGTAAAGTACAAACCAGGACCAGAGGAAACAGACCCTT38051.4604731856355457No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA35401.358758233153701No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA34431.321526722245252No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA28751.1035112769256752No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA27341.0493912456051464No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG22330.8570924109130549No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT20240.7768719389556753No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT20240.7768719389556753No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT19800.7599834185435954No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG19690.7557612884405754No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT19050.7311961678411866No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA18120.6954999769701995No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG17770.6820659266424086No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT16100.6179663150783781No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC15200.5834216142354874No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT14980.5749773540294475No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC14940.5734420339919857No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT12860.4936053920439716No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCAAAAGTGAAACATTTTGTCC12270.4709594214914099No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG12130.46558580136029354No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG12110.4648181413415627No Hit
GCTGCCCCCACCATGAGGATGTAGAACCATGTCGTCAGTGTTGCGTGTGG12050.46251516128536996No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA11560.44370749082646277No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG10980.4214453502832665No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG10810.4149202401240538No Hit
AAGGGTGCAGTTATGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTG10650.40877895997420666No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT10320.3961125696651467No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG9950.38191085931862495No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC9490.36425467888781415No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT9370.35964871877542876No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG9310.35734573871923603No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA8950.3435278583820798No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA8810.3381542382509634No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC8810.3381542382509634No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA8590.32970997804492347No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA8110.31128613759538176No Hit
AAGGGTGCAGTTATGCCTCAGTGTACACTCAAAATGCCGTTTTCTTGTAG7770.29823591727695636No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC7760.29785208726759094No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7390.2836503769210692No Hit
TCTGGATCAGAGTCAGTGGTCATGTAGAACCATGTCGTCAGTGAAAACCC7330.2813473968648765No Hit
GCTGCCCCCACCATGAGTAGGAAGGCTTTGCGTGTGGAGTATTTGGATGT7160.27482228670566383No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC7010.269064836565182No Hit
GTTCAAATGAGTAGACACAGCTTGTTCTACAGGTCTTCTCTGTTTCAGGG6900.264842706462162No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCTTAGACCAAGTCTTACCCAA6750.2590852563216802No Hit
CTCGATAATCTGGATGACTCATTATTGTTGTAGCACAACTAAAAGAAAAG6710.25754993628421846No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT6710.25754993628421846No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATTATGATGTAGAACCATGTC6680.25639844625612207No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA6160.23643928576911857No Hit
ATCTCCGCAAGAAAGGGGAGATAGACACTGCCCAACAACACCAGTGTAGA6050.2322171556660986No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA5990.2299141756099059No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5870.22530821549752045No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA5820.22338906545069317No Hit
TGCCCTGACTTTCAACTCTGTGCTACCCTACAAGCAGTCACAGCACATGT5810.22300523544132775No Hit
CCACACAGCAAAGCAGAAACCATGTCGTCAGTGTAGACGTTAACTGGCAA5500.21110650515099874No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC5300.20342990496368968No Hit
GCTGCCCCCACCATGAGGATGTAGAACCATGTCGTCAGTTTGCGTGTGGA5270.20227841493559331No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTGGCACAACTAAAAGAA5220.2003592648887661No Hit
GTTCAAATGAGTAGACACAGCTTTTCTATACAAAGTTGTCAGGGCACGGT5210.19997543487940062No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAATGTAGAACCATGTCGTCA5180.19882394485130425No Hit
TCCCTGCTTCTGTCTCCTACCGTACCATTTTGGGTTTTGGGTCTGTAGAA5150.19767245482320792No Hit
TCATTACACCAGTTCGTCCCTGTCTGTTTCCTCTGGTCGTGGTAAAAAGA5090.19536947476701516No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC4970.19076351465462976Illumina Single End PCR Primer 1 (95% over 22bp)
AATAGTTGTTTTAGAAGATATTTGCAAGCATTATGATGTAGAACCAACTA4900.18807670458907158No Hit
TCATTACACCAGTTCGTCCCTGCTACCGGTGGTAAAAAGATAACCTCAGA4800.18423840449541706No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTGGGAAGAAGCTCT4740.18193542443922436No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG4680.17963244438303164No Hit
TTCATGGTGTTTTATCCCTCTTGATTCGAACCTGCGCGGGGAAACCCCAA4600.17656180430810803No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC4590.17617797429874257No Hit
GTTCAAATGAGTAGACACAGCTTCTTCTCTGTTTCAGGGCACGGTAGAAG4580.17579414428937712No Hit
AAGGGTGCAGTTATGCCTCATACGGTAGCAGACACTCAAAATGCCGTTTT4550.17464265426128078No Hit
TCCCTGCTTCTGTCTCCTACCGTGCCATTTTGGGTTTTGGGTCTGTAGAA4550.17464265426128078No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCAGCACAACTAAAA4530.17387499424254987No Hit
GTTCAAATGAGTAGACACAGCTTCTGCTACCGCAGGGCACGGTAGAAGTT4510.17310733422381894No Hit
CACACACAGGTAACGGCTGCGGTAGCAGAGACTTGGTATGCCTTTAACTG4380.16811754410206808No Hit
GCTGCCCCCACCATGAGTAGGAAGGCCTTGCGTGTGGAGTATTTGGATGT4300.16504690402714445No Hit
CCATGGGACTGACTTTCTGCTACCCAAGCAATGGATGATTTGATGTAGAA4270.1638954139990481No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTCTTGTAGAACCATGTCGT4270.1638954139990481No Hit
CACACACAGGTAACGGCTGAACATGCCTTTAACTGTCAAACTGTCATGTA4250.1631277539803172No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG4210.16159243394285538No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTTGTAGAACCATG4170.16005711390539357No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA4140.15890562387729723No Hit
TCATTACACCAGTTCGTCCCTGCTACGTGGTAAAAAGATAACCTCAGAAT4060.1558349838023736No Hit
CACACACAGGTAACGGCTGCACTCTACATGCCTTTAACTGTCAAACTGTC4040.1550673237836427No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGTCATTTTCAGCAGGCTGTAGAA3970.15238051371808453No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA3830.1470068935869682No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG3820.14662306357760274No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC3770.14470391353077547No Hit
CCACACAGCAAAGCAGAGACGTTAACTGGCAATTGTGATGTAGAACCATG3670.14086561343712095No Hit
AACAGAGAATCTCCATTTTAGCACGTAGCAGAGACTTTGTCATATTTTTC3620.1389464633902937No Hit
AAGGGTGCAGTTATGCCTCACACTCAAAATGCCGTTTTCTTGTAGAACCA3610.13856263338092825No Hit
TGCCCTGACTTTCAACTCTGTGCTACAAGCAGTCACAGCACATGTAGAAC3580.13741114335283192No Hit
TCATTACACCAGTTCGTCCCTACGTGGTAAAAAGATAACCTCAGAATAAG3540.13587582331537007No Hit
TCCGTCTACTCCCACGTTCTACGGTAGCAGAGAGATGGCAGAAGCTGCTG3500.13434050327790828No Hit
CACACACAGGTAACGGCTGCTGGTCTACATGCCTTTAACTGTCAAACTGT3500.13434050327790828No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC3460.1328051832404465No Hit
ATGAGGTACTCGTCGGCATCGGTAGCAGAGACTTGGCTATAGGAGCAGGG3440.13203752322171555No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAAGGCAATCTGA3370.1293507131561574No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT3360.12896688314679194No Hit
TTCTCTTCCGCACCCAGCCTGTCGACATGCAATTGGGAAGAAGCTCTGCA3280.12589624307186834No Hit
GCCTCAAAGACAATGGCTTTGCCTGATACAGATGTAGAACCATGTCGTCA3250.12474475304377199No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA3220.12359326301567561No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAACCATGTCG3190.12244177298757926No Hit
GCTGCCCCCACCATGAGGGCTTTGCGTGTGGAGTATTTGGATGTAGAACC3150.12090645295011745No Hit
TCATTACACCAGTTCGTCCCTAAAACCAAGGCGTGGTAAAAAGATAACCT3140.12052262294075201No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT3140.12052262294075201No Hit
GCTGCCCCCACCATGAGGCTTTGCGTGTGGAGTATTTGGATGTAGAACCA3110.11937113291265564No Hit
TCTGGATCAGAGTCAGTGGTCATGTAGAACCATGTCGTCAGTGTAGATCT3100.11898730290329018No Hit
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG3070.11783581287519383No Hit
TTCTCTTCCGCACCCAGTACGGTAGCAGAGACCTGTGGGAAGAAGCTCTG3050.11706815285646294No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG3030.11630049283773201No Hit
GGAGAGCTGTAAATTCTGGCTTGATCGTTAACTTATTCACTTGTCGTCGT3030.11630049283773201No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG3020.11591666282836656No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA3010.11553283281900112No Hit
ATGAGGTACTCGTCGGCATCTAAGATTGTCACAGTCGCATGGCTATAGGA2930.11246219274407751No Hit
AGAATGGTCCTGCACCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC2920.11207836273471204No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT2920.11207836273471204No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC2910.11169453272534659No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT2880.11054304269725024No Hit
CAGCCAGACTGCCTTCCGGTAGCAGAGACCTGTGGGAAGCGAAATGTAGA2870.11015921268788478No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT2860.10977538267851934No Hit
CACACACAGGTAACGGCTGATTCTACATGCCTTTAACTGTCAAACTGTCA2790.10708857261296116No Hit
CACACACAGGTAACGGCTGAGACATGCCTTTAACTGTCAAACTGTCATGT2730.10478559255676846No Hit
TCCGTCTACTCCCACGTTCTTACGTAGCAGAGAGATGGCAGAAGCTGCTG2720.10440176254740302No Hit
CACACACAGGTAACGGCTGAGACCAAATGCCTTTAACTGTCAAACTGTCA2720.10440176254740302No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT2710.10401793253803754No Hit
TCATTACACCAGTTCGTCCCTGTGGTAAAAAGATAACCTCAGAATAAGAA2700.1036341025286721No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT2650.10171495248184484No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTCTACAA2610.10017963244438305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)