FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480701
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT260815.425618003707086No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA246575.129383962171912No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA227224.7268468341026955No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC172623.591005635519793No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA114062.372784745611097No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA109712.282291902866855No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG106992.22570787246126No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC104472.173284432526664No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC103222.1472807420829163No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC102102.1239814354453186No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG98542.0499229250615247No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG96542.0083170203515284No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG94871.9735760899186814No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG91981.9134555576127366No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT91021.8934847233519383No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG88851.848342316741592No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT84171.7509844997202002No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC83681.7407910530662511No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG81941.7045939159685541No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG79031.6440573246155092No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA75091.5620936923368165No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC65721.367170028770483No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA55441.1533156785611014No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT54371.1310565195412534No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA46070.958392014994768No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT43810.9113773426724721No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT42510.8843335046109744No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA36870.7670048533287844No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT35990.748698255256386No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC33390.6946105791333905No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT31400.6532127039469441No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA27950.5814425183222003No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC27950.5814425183222003No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG27820.5787381345160505No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA27200.5658403040559516No Hit
TGACCAATGGCTAAGTGAAGATGACACTGACGACTTGTTTTTCCTTTCCT25350.5273548421992049No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAACGTATCATCAT25240.5250665174401551No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC24110.5015591812790071No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC24070.5007270631848072No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG23350.4857489374892085No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA22800.47430731369395945No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT21890.4553766270509111No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT20790.43249337946041305No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA19820.4123145156760648No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG19800.4118984566289648No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA19370.40295318711631556No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG17670.3675881681128186No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG17290.35968304621791924No Hit
CAGAGGGGAAAAATATGACAAAACACTGACGACATGGTGCTAAAATGGAG15870.33014285387382175No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG14690.3055953700949239No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT13420.27917562060407614No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC12370.257332520631328No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC11430.23777774541762967No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT11220.23340912542308004No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGAAAGCTGTGTCTACTCATTTGA10820.22508794448108074No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT10610.22071932448653112No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCAAGAGGGATAAAACA10360.2155185863977816No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA10210.21239814354453182No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG9540.19846016546668302No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGGTGCAGATAATGACAAGGAATA9410.19575578166053328No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9370.19492366356633334No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC8920.18556233500658414No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA8190.17037617978743544No Hit
CAGTCAGAGGCGCTATGTGTAGAAGTATGGAACAGTTTTCTTAACCATAT8140.1693360321696855No Hit
TGACAGTTTGACAGTTAAAGGCATGTTGGTCTCAGCTTAATTAAACCTCA8000.16642361883998577No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCACTGACTCTGATCCAGAA7940.16517544169868587No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA7710.16039076265703628No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCGCTACCTGTTAAAGAATCATCT6990.14541263696143755No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC6770.14083598744333795No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5920.12315347794158947No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAACTTCAAATGTTA5610.11670456271154002No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGATCTGGGTCTTCAGTGAAC5610.11670456271154002No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC5320.11067170652859053No Hit
CAGCCTCTGGCATTCTGGGAGCTTTATCTGGACCTGGGTCTTCAGTGAAC5300.11025564748149057No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA5060.105262938916291No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC4940.1027665846336912No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAACG4810.10006220082754144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)