FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449461
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA180044.005686811536484No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA165943.691977724429928No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC156713.486620641167977No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG152383.39028302789341No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA113642.5283617488502896No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA107192.3848565281526097No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA96012.136114145609964No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC90912.0226449013373795No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG83371.854888410785363No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA75361.6766749506631276No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC72241.6072584718140173No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA70031.558088465962564No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG68681.5280524895374683No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT68281.5191529409670694No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT67901.5106983698251908No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC65491.457078589688538No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT64091.4259301696921423No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG62381.3878845995536877No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGGTGGAATAT58911.3106810157054785No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG58181.2944393395645006No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG55401.2325874770002292No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT50291.1188957440133849No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA47611.0592687685917133No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG45061.0025341464554212No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT43320.9638211101741864No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC42220.9393473516055899No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC40820.9081989316091941No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT39150.8710433163277792No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT37440.8329977461893244No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC37310.8301053929039449No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT37090.8252106411902256No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA35860.7978445293362495No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA35710.7945071986223499No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT30540.6794805333499458No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTT30180.6714709396365869No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT27250.6062817463584159No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG26180.5824754539325993No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA25650.570683552076821No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG25280.562451469649202No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC23940.5326379819383662No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG23540.5237384333679674No Hit
TGTGCTGTGACTGCTTGTAGTCTCTGCTACCGTAACAGAGTTGAAAGTCA21870.48658281808655257No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG21380.47568087108781404No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG20750.46166408208943605No Hit
GCCTGCTGAAAATGACTGAATATAAACTTATGGTAGTTGGAGCTGGTGGC18870.4198362038085618No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG18450.41049167780964313No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16850.374893483528048No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT16770.3731135738139683No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC16360.36399153652930955No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT15940.3546470105303908No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG15920.3542020331018709No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG15470.3441900409601723No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC15370.3419651538175726No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG15280.3399627553892329No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT15280.3399627553892329No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC14140.3145990419635964No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT11680.25986681825564395No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTGGGTGCGGAAGA10980.24429260825744614No Hit
CAGCCTCTGGCATTCTGGAAGACCAAGTCTAGAGCAGTCAGAGGACCAGG10920.24295767597188633No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA10820.24073278882928661No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATAGGTGGAATATCTCG10770.2396203452579868No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC10380.23094328540184797No Hit
GCCTGCTGAAAATGACTGAATATAAACCTGTGGTAGTTGGAGCTGGTGGC10290.22894088697350828No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT10170.22627102240238864No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT9830.21870640611754968No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT9680.21536907540365013No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGTAGAACCATGAAGCTGT9620.21403414311809035No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC9480.21091930111845078No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACATGTAGAACGGAGATAACACAGG9430.20980685754715092No Hit
CAGAGGGGAAAAATATGACAAACACTGACGACATGGTGCTAAAATGGAGA9220.20513459454769156No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCGG8930.19868242183415244No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA8880.19756997826285264No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT8540.19000536197801365No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC8450.18800296354967394No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATATA8440.187780474835414No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA7830.17420866326555584No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCGTGCTAAAATGGAGATTCTC7590.1688689341233166No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG7110.15818947583883808No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC7100.15796698712457813No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAG6930.15418467898215862No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG6820.15173730312529896No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGGCTAATGAG6770.15062485955399912No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6680.14862246112565938No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC6470.14395019812620005No Hit
GGAATCCTATGGCTTTCCAACCCAGCAACAGAGGAGGGGGAGACCAAGTC6470.14395019812620005No Hit
TCACAATTGCCAGTTAACGTCTCCCTTCTCTCTCTGTCATAGGGACTCTG6360.14150282226934038No Hit
GCAGCTTCTGCCATCTCTCTGGTAGCAGAACGTGGGAGTAGACGGAAGAC6320.1406128674123005No Hit
CAGAGGGGAAAAATATGACAAATACGGTGCTAAAATGGAGATTCTCTGTT6060.1348281608415413No Hit
CAGAGGGGAAAAATATGACAAACAGTGCTAAAATGGAGATTCTCTGTTAG5740.1277085219852223No Hit
TCACAATTGCCAGTTAACGTCTTTCTTCTCTCTCTGTCATAGGGACTCTG5610.12481616869984269No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG5530.12303625898576295No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC5470.12170132670020313No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG5220.1161391088437039No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCCTCATACCAGGACCA5020.11168933455850452No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATAGGTGG5010.11146684584424456No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4950.11013191355868475No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT4840.10768453770182507No Hit
TGTTCATTCATGATCCCACTGCTACCGTAACCAAGTGTGAGTGACTGGTG4730.10523716184496543No Hit
GGAATCCTATGGCTTTCCAACCTAAGAAGGCAGGGGAGTAGGACCAGGAA4570.10167734241680591No Hit
CAGAGGGGAAAAATATGACAAGTCTCTGCTACCGTGTGCTAAAATGGAGA4520.10056489884550607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)