FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449461
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC183134.074435824242816No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA170313.789205292561535No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA162753.621003824580998No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG105512.3474784241569346No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT105022.336576477158196No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA86711.9291996413481924No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC75921.689134318661686No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT70741.5738851646750218No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA70391.566098059675923No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG65271.4521838379748186No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC65271.4521838379748186No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC64761.4408369135475603No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG64011.4241502599780627No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG61671.37208790084123No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA60781.3522864052720927No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT59621.326477714417936No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG59521.3242528272753364No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT52221.1618360658655589No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT50731.1286852474408235No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTAAGCAGCTGGGG47821.0639410315911726No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA47431.0552639717350338No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA45941.0221131533102983No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG44720.9949695301705821No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA44430.9885173574570429No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA40640.9041941347525148No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC40350.8977419620389756No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT39010.8679284743281397No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT37190.8274355283328254No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA36720.8169785587626068No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT36010.8011818600501489No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA35840.7973995519077295No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT35790.7962871083364296No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA34750.7731482820533928No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCGCCTTTCCTTGCCTCT31110.6921623900627641No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT30490.678368089778646No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC30330.6748082703504864No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG26110.5809180329327794No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGTCTCT25700.5717959956481208No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT23890.5315255383670664No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA23610.5252958543677873No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC23600.5250733656535272No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT22670.5043819152273501No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT22470.4999321409421507No Hit
TGCCCTGACTTTCAACTCTGTTACGGTAGCAGAGACTACAAGCAGTCACA21120.46989616451705485No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG20250.4505396463764376No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT18440.4102691890953831No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT17640.3924700919545856No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG17380.3866853853838264No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC16900.3760059270993479No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG16730.3722236189569284No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT16360.36399153652930955No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG16320.3631015816722697No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT16170.35976425095837017No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC15930.3544245218161309No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC15800.35153216853075125No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT15410.3428551086746125No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTATATGAACTTGTCTTCC15300.34040773281775283No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA15180.3377378682466332No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT11600.25808690854156424No Hit
TCATTACACCAGTTCGTCCCTTCCAGCTTTACAGTGAATTGCTGCAACAT11450.2547495778276647No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT11090.24673998411430578No Hit
CCTGGTCCTCTGACTGCTCTAGACTTGGTCTTCCAGAATGCCAGAGGCTG11010.24496007440022605No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC10870.24184523240058647Illumina Single End PCR Primer 1 (100% over 21bp)
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT10690.23784043554390702No Hit
TTCTCTTCCGCACCCAGAACCATGTCGTCAGTGTGGGAAGAAGCTCTGCA10650.23695048068686714No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG10400.23138826283036792No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA10060.223823646545529No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG10050.22360115783126902No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG10000.22248871425996916No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCCCTA9910.22048631583162945No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA9860.2193738722603296No Hit
CTTGGGCCTGTGTTATCTCCGTTCTACATGTGCTGTGACTGCTTGCCTCT9470.2106968124041908No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA9450.21025183497567088No Hit
AACAGAGAATCTCCATTTTAGCACCATGTCGTCAGTGTTTGTCATATTTT9400.20913939140437102No Hit
GTTCAAATGAGTAGACACAGCTTCATGGTTCTACAGGTCTTCTCTGTTTC9390.20891690269011104No Hit
CACACACAGGTAACGGCTGAAGGAACTCAAAGTACATGAACTTGTCTTCC9280.20646952683325137No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG8880.19756997826285264No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA8530.1897828732637537No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT8520.18956038454949373No Hit
TCCGTCTACTCCCACGTTCTAAGAGGGTGACAGAAAGGTAAAGAGGAGCA8380.18644554254985415No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTTCAGTACTCCCCTG8080.17977088112205508No Hit
GGCTCCTTATCTCCCCTCCTCGTATCTCCCTTCCCTGATTACCTTTGCGA7890.17554359555111565No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT7650.17020386640887641No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7460.16597658083793698No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7400.16464164855237717No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTAGCATCTTGTTCTGTTT7300.16241676140977748No Hit
AACAGAGAATCTCCATTTTAGCACGCAGAGACTTTGTCATATTTTTCCCC7250.16130431783847762No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA7080.15752200969605817No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7070.1572995209817982No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG6980.15529712255345848No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATTTTGTTCTGTTT6860.15262725798233884No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT6610.1470650401258396No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA6490.14439517555471998No Hit
CCCCCTCCTCTGTTGCTGGGTTGGAAAGCCATAGGATTCCTGTAGAACCA6460.1437277094119401No Hit
TCCGTCTACTCCCACGTTCTGCTACCAGAGAGATGGCAGAAGCTGCTGTA6240.13883295769822077No Hit
AACAGAGAATCTCCATTTTAGCACCGTATTTGTCATATTTTTCCCCTCTG6010.13371571727024148No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACG5960.13260327369894162No Hit
AACAGAGAATCTCCATTTTAGCACTGTTTGTCATATTTTTCCCCTCTGTG5710.12704105584244238No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG5560.12370372512854286No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA5490.12214630412872307No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC5470.12170132670020313No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA5400.12014390570038334No Hit
GCTGCCCCCACCATGAGTGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG5260.11702906370074377No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC5220.1161391088437039No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCA5220.1161391088437039No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC5180.11524915398666402No Hit
GTTCAAATGAGTAGACACAGCTTGAGTGAGAGAGAGACAGAGATTTCATA5160.1148041765581441No Hit
AAGGGTGCAGTTATGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTG4950.11013191355868475No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCGGGGC4920.10946444741590483No Hit
AGGGATGCAAAGGCCTCAGTGTACATCTCGCTGGCAGGGATGTAGAACCA4860.10812951513034502No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGCCTA4780.10634960541626526No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4740.10545965055922539No Hit
CCACCAGTCACTCACACTTGGTTACGGTAGCAGTGGGATCATGAATGAAC4720.10501467313070545No Hit
AACAGAGAATCTCCATTTTAGCACACGGTAGCAGAGACTTGTCATATTTT4680.10412471827366555No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC4670.1039022295594056No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT4610.10256729727384578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)