FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523355
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC216294.13275883482531No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA206433.944358991506721No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA194293.7123940728568563No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT137632.6297637358962844No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA136802.6139045198765656No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG131702.516456325056606No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA124002.3693286583676474No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA123492.3595838388856514No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC123242.354806966590555No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG121332.318311662256021No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC115782.2122650973048885No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC111282.1262813959931592No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT105662.0188973067994No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT105542.016604408097754No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC93201.7808179916118123No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT91801.7540675067592741No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT91161.7418387136838285No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT87131.6648355322868797No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG79831.5253508612700748No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG75581.4441440322534418No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG75311.4389850101747381No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA72051.3766945954466854No Hit
GACCCAAAACCCAAAATGGCACGGTAGGAGACAGAAGCAGGGAAGACCAA62021.1850464789674313No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC57391.0965788040622522No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT56621.0818660373933564No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA52240.9981752347832733No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA50250.9601513313143086No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATGAGGTATAT47580.9091343352026826No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA45660.872447955976345No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG45190.8634674360615644No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC45030.8604102377927029No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC38900.7432813291169473No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG38090.7278042628808361No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG36930.7056395754315904No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT30750.5875552922968158No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG30220.577428323031212No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT27540.5262202520277823No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT24670.4713817580800795No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT24430.4667959606767873No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA23720.45322964335871446No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT23380.4467330970373838No Hit
TCAAATCATCCATTGCTTGGAGACGCAGAAAGTCAGTCCCATGGAGACGC22770.4350775286373494No Hit
GGGGTCAGAGGCAAGCAGGACATGTAGAACGGAGATAACACAGGCCCAAG22110.4224665857782958No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG21830.4171164888077882No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG20470.39113030352246564No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC19570.3739335632601198No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGAGACTCTG19550.3735514134765121No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGCGGTGGAT19410.3708763649912583No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC18040.3446991048141319No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA17880.3416419065452704No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC17360.3317060121714706No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC17310.3307506377124514No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16840.3217701177976708No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC16460.3145092719091248No Hit
CAGAGGGGAAAAATATGACAAATGTGCTAAAATGGAGATTCTCTGTTAGA16370.3127895978828902No Hit
CAGCCTCTGGCATTCTGGGAACTTCATCTGGACCTGGGTCTTCAGTGAAC15770.30132510437465965No Hit
TGAGCAAGAGGCTTTGGAGTACATGGTTCTACATTCTTATTCTGAGGCAT15120.2889052364074099No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC13900.2655940996073411No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCGACTG13240.25298315674828753No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT12490.2386525398629993No Hit
GGAATCCTATGGCTTTCCAACCCGACATGGTTCTACAGCAACAGAGGAGG11820.22585052211214185No Hit
TCAAATCATCCATTGCTTGGATACGTAGCAGAAAGTCAGTCCCATGGAGA11570.2210736498170458No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGATGATAAAAG11570.2210736498170458No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCATAGGCTCCCAG11260.21515032817112667No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAGGCCAGCCCCTCAGGGCAACTG10720.20483228401371917No Hit
ATACGGCCAGGCATTGAAGTCTCATAGAAGCCAGCCCCTCAGGGCAACTG10340.19757143812517314No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG10340.19757143812517314No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGAACCA10200.19489638963991937No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG10080.19260349093827325No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG9800.18725339396776566No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT9770.18668016929235415No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGTAAGGAAAGGTGATAAAAG9630.18400512080710035No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAT9590.18324082123988497No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA9300.17769964937757354No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC8990.17177632773165444No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG8280.1582100104135816No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAAACATTATGACA8200.15668141127915086No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGGAAGACATTATGACA8030.15343313811848555No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCATCTCC7820.14942056539060483No Hit
GGGGTCAGAGGCAAGCAGTCACAGCAGGAGATAACACAGGCCCAAGAGAC7710.14731874158076258No Hit
CAGAGGGGAAAAATATGACAAAGAAAACTATATAAGATATTATTTTATTT6240.11923073248559773No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTTTCATCTGGACCTGGG5930.11330741083967862No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTAGC5890.11254311127246325No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT5790.1106323623544248No Hit
TCAAATCATCCATTGCTTGGAGACGCAGAAAGTCAGTCCCATGGAGACCA5670.1083394636527787No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5600.1070019394101518No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCGCTAATGAGTTTGTACTGA5530.10566441516752492No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAGGACCA5250.10031431819701733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)