FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523355
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA214074.090340208844856No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC208973.992892014024897No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA197153.7670414919127553No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC166943.189804243773347No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA118532.2648106925509457No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG107292.0500425141634264No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA100711.9243152353564978No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT100371.9178186890351674No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT96071.835656485559515No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG94961.8144471725692886No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT94311.802027304602039No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA91681.7517746080576282No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG89811.7160436032903097No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC87681.675344651336091No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA85701.6375118227589303No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT84021.6054112409358847No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC80981.5473244738275167No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT79491.5188543149487441No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT78331.4966896274994983No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG75841.4491119794403418No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA72141.3784142694729198No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC65981.2607121361217528No Hit
TCCCTGCTTCTGTCTCCTACCGTGCCATTTTGGGTTTTGGGTCTGTAGAA61401.173199835675593No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA57161.0921840815507637No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA56891.08702505947206No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT54561.0425046096817647No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT49970.954801234343801No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG48010.9173505555502479No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT45590.8711104317337179No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA44580.85181186766153No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACTTTTCCTTGCCTCT41750.7977376732810425No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG39370.752261849031728No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA38620.7379312321464399No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA33990.6494635572412607No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT31360.5992108606968501No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG30430.5814408957590927No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT27170.5191504810310401No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC26930.5145646836277479No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA25060.4788336788604294No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT24780.47348358188992173No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT24420.46660488578498344No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC23570.45036351998165686No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC22620.4322114052602918No Hit
CCATGGGACTGACTTTCTGCGTCTCCATGGGACTGACTTTCTGCGTCTCC22150.4232308853455112No Hit
CTTGGGCCTGTGTTATCTCCGTTCTACATGTCCTGCTTGCCTCTGACCCC21560.4119574667290845No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT19840.37909258533882356No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT19780.37794613598800053No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCTCCTTGCCGTC19350.3697299156404353No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACTGTCCCCCTTGCCGTC18600.3553992987551471No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAGCATACT17860.3412597567616627No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG17730.3387757831682128No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG17450.3334256861977052No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG16770.3204325935550439No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAT16320.31183422342387096No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT16310.31164314853206715No Hit
AACAGAGAATCTCCATTTTAGCACATTTGTCATATTTTTCCCCTCTGTGT16260.31068777407304793No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC15950.3047644524271288No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT15810.302089403941875No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAAGAGCA15120.2889052364074099No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT14610.2791604169254139No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT14520.27744074289917936No Hit
AAGGGTGCAGTTATGCCTCAGAATAAGAATGTAGAACCATGTACTCCAAA14330.27381031995490634No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGGGTTGGAAAGCCATAGGAT11610.22183794938426116No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA11380.2174432268727728No Hit
CCATGGGACTGACTTTCTGCTACGTATCCAAGCAATGGATGATTTGATGT11210.21419495371210748No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGCGACAGAAAGGTAAAGAGGAGCA10650.2034947597710923No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA10310.19699821344976165No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCCCTGACTGTACCACCATCCAC10240.19566068920713475No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG10050.19203026626286174No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT9900.1891641428858041No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT9590.18324082123988497No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA9560.18266759656447343No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG9430.1801836229710235No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC9360.1788460987283966No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGGCAGAGATTTCA9270.17712642470216203No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA9150.1748335260005159No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA9120.1742603013251044No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCATACCCC8820.1685280545709891No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8440.16126720868244307No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATATTGCAAATGCTATC8310.1587832350889931No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT8250.15763678573817008No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCT8090.15457958746930858No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA8070.1541974376857009No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAATTAACTTCAGAACACCA7920.15133131430864327No Hit
TCATTACACCAGTTCGTCCCTTCCAGCTTTACAGTGAATTGCTGCAACAT7600.14521691777092033No Hit
CTTGGGCCTGTGTTATCTCCTGCTGTGACTGCTTGCCTCTGACCCCTGTA7600.14521691777092033No Hit
GGAGAGCTGTAAATTCTGGCTTATAAAGTGTTCATACATACCCCATTTCT7300.13948467101680503No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTACCCCCAGGGAGCACT6330.12095040651183232No Hit
AAGACCCAGGTCCAGATGAAACTTGGTCTAAGACCCAGGGCAGCTACGGT5810.1110145121380325No Hit
CCAACTGGCCAAGACCTGTACGGTAGCGATGGTGAGCAGCTTGTAGAACC5740.1096769878954056No Hit
CCATGGGACTGACTTTCTGCGTCTCCAAGCAATGGATGATTTGATGTAGA5630.10757516408556334No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC5600.1070019394101518No Hit
GGCCTCAGTACAAACTCATTAGCGACTTGGTCTGGACCTAATTACGAGTG5500.10509119049211338No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGGTACAGTCTA5400.10318044157407497No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5290.10107861776423269Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)