FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465320
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC212794.572982033869166No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA193914.167239748989942No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT137442.9536662941631566No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA127652.743273446230551No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT115222.4761454482936474No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG112272.412748216281269No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA109992.3637496776411933No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT107842.317544915327087No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC105332.263603541648758No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC105262.2620992005501592No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG96062.064385799020029No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA95992.0628814579214305No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG94852.0383821886013926No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA90851.952419840110032No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA83661.7979025186968107No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG75281.6178113986074099No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT69591.4955299578784493No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT66821.436001031548182No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC65991.4181638442362245No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC64201.3796956932863405No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC63321.3607839766182412No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA57321.2318404538812No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA50761.0908622023553682No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA49861.0715206739448122No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG46490.9990973953408406No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC45240.9722341614372904No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT45100.9692254792400928No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG37990.8164274047966991No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT37670.8095504169173903No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG33770.7257371271383134No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG33330.7162812688042637No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT30430.6539585661480272No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC30270.6505200722083727No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACGTGCTAAAATGGAGATT29050.6243015559185077No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28740.6176394739104273No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGGAGCATGTAT28370.6096879566749763No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT27100.5823949110289693No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC26870.577452075990716No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC26410.5675664059142096No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG25500.548009971632425No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA24760.5321069371615232No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC22130.4755866930284535No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG20050.430886271812946No Hit
CAGAGGGGAAAAATATGACAAAAATAATTTTCTTCCAGGCTTGTGCTAAA19900.4276626837445199No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC19580.42078569586521103No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA19480.41863663715292704No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG19130.41111493165993296No Hit
GTAGAGGAGCCGTCAAATCCATTGGGGTTTCAAGAGGGATAAAACACCAT17090.36727413392933894No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA16710.3591077108226597No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAGGACCA16210.34836241726123957No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT16040.3447090174503567No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC15620.33568297085876386No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG15370.33031032407805383No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCGTGCTAAAATGGAGATTCTC15370.33031032407805383No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG13670.2937763259692255No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAAGACCA12620.2712112094902433No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG12490.268417433164274No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT12310.2645491274821628No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCTTCCCT12000.25788704547408237No Hit
CTGGTGTTGTTGGGCAGTACATGGTTCTCCCCTTTCTTGCGGAGATAGAC11660.2505802458523167No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC10940.23510702312387177No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGGATGCCGACGAGTACCTCATAGAC10480.22522135304736526No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG10210.2194188945241984No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGATGCGGGTGCCGGGC10140.21791455342559957No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG9840.21146737728874754No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTAGTG9540.20502020115189548No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCAGGC9190.1974984956589014No Hit
CAGCCTCTGGCATTCTGGGAGTTTCATCTGGACCTGGGTCTTCAGTGAAC9180.197283589787673No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT9080.19513453107538897No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC8960.19255566062064816No Hit
TCAAATCATCCATTGCTTGGTACCGGTAGGCAGAAAGTCAGTCCCATGGA8840.18997679016590732No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG7870.16913092065675234No Hit
TCAAATCATCCATTGCTTGGTACCGTAGCAGAAAGTCAGTCCCATGGAGA7650.16440299148972748No Hit
TGACAGTTTGACAGTTAAAGGCATTGGTCTCAGCCGTTACCTGTGTGTGA7600.1633284621335855No Hit
GGAATCCTATGGCTTTCCAACCCGACATGGTTCTACAGCAACAGAGGAGG7330.15752600361041866No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGAACGCGGGTGCCGGGC7200.1547322272844494No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA7140.153442792057079No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC7040.15129373334479498No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACACGGGTGCCGGGC7040.15129373334479498No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGAACTCAGCCGTTACCTGTGTGT6970.14978939224619617No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6850.14721052179145533No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGG6800.14613599243531333No Hit
ACTTCTACCGTGCCCTGATGGGTGAAGAAGACATGGACGACGTGGTGGAT6620.1422676867532021No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT6300.13539069887389324No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCT6190.1330267342903808No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACGCTACCTGTTAAAGAATCA6010.1291584286082696No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT5790.12443049944124472No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA5400.11604917046333706No Hit
GGGGTCAGAGGCAAGCAGGCAAAGACCAGACAAAGTGGAGATAACACAGG5330.11454482936473825No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATAACA5260.11304048826613942No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT5170.11110633542508382No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA4770.10251010057594774No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4690.10079085360612053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)