FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465320
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA202964.361729562451646No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC176333.7894352273704115No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA174763.7556950055875524No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC116742.5088111407203644No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC113512.4393965443135905No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA109322.34935098426889No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC108322.32786039714605No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA107002.2994928221439013No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG99632.1411071950485687No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG99122.1301469956159202No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT97632.0981260208028885No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA95522.0527808819736952No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT88941.911372818705407No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA83211.7882317544915327No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG76931.6532708673600964No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT74671.6047021404624773No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA74581.6027679876214218No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA72791.564299836671538No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC62471.342516977563827No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA58961.267085016762658No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT50961.0951603197799364No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA50551.0863491790595718No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT47491.020587982463681No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT45400.9756726553769449No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA44860.9640677383306111No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT37410.8039628642654516No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT34620.7440041261927276No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT34090.7326141150176223No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT31650.6801770824378922No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC30500.6554629072466259No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA29740.6391300610332674No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT29600.6361213788360698No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG29050.6243015559185077No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG28730.6174245680391989No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT28680.6163500386830568No Hit
AACAGAGAATCTCCATTTTAGCACGTAGCAGAGACTTTGTCATATTTTTC28670.6161351328118284No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT27540.591850769363019No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT27090.5821800051577409No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT25370.5452161953064557No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC25140.5402733602682026No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA24930.5357603369724061No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT24530.52716410212327No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC23780.5110461617811398No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATAGTACAT22190.476876128255824No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG19940.4285223072294335No Hit
AACAGAGAATCTCCATTTTAGCACAAGCCTGGAAGAAAATTATTTTTGTC19870.42701796613083476No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA18550.39865039112868567No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG17880.3842516977563827No Hit
TTCATGGTGTTTTATCCCTCTTGAAACCCCAATGGATTTGACGGCTCCTC16700.3588928049514313No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC16190.3479326055187828No Hit
AACAGAGAATCTCCATTTTAGCACGCAGAGACTTTGTCATATTTTTCCCC15100.324507865554887No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA14100.3030172784320468No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC13340.2866844322186882No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG12580.27035158600532966No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAAGGC12150.26111063354250835No Hit
ATCTCCGCAAGAAAGGGGAGAACCATGTACTGCCCAACAACACCAGTGTA11730.2520845869509155No Hit
TGCCCTGACTTTCAACTCTGTTTCCTTCCTCTTCCTACAGTACTCCCCTG11630.2499355282386315No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGTCGTC11380.24456288145792143No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG11040.23725608183615574No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC10750.23102381157053212No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA10630.22844494111579128No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTTAGTCT10550.22672569414596408No Hit
ATGAGGTACTCGTCGGCATCCAGAGACTTGGCTATAGGAGCAGGGGTGTA10310.2215679532364824No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC9910.21297171838734635No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT9850.21168228315997595No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTTTA9680.20802888334909309No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA9280.19943264849995704No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG9000.19341528410556177No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCTGC8880.19083641365082094No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT8820.18954697842345053No Hit
CCATGGGACTGACTTTCTGCCTACCGGTACCAAGCAATGGATGATTTGAT8800.1891171666809937No Hit
TGCCCTGACTTTCAACTCTGTCTCTTTCCTCTTCCTACAGTACTCCCCTG8470.18202527293045645No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC8160.17536319092237598No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC7760.1667669560732399No Hit
CCATGGGACTGACTTTCTGCTACGGTACCAAGCAATGGATGATTTGATGT7580.1628986503911287No Hit
CACACACAGGTAACGGCTGAGACCAATGCCTTTAACTGTCAAACTGTCAT7550.1622539327774435No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGGGTTGGAAAGCCATAGGAT7390.15881543883778904No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT7100.1525831685721654No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGTCAGAGGCTGCTCCCCGC7050.1515086392160234No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA7020.15086392160233816No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6990.15021920398865296No Hit
CACACACAGGTAACGGCTGAGTTCTACATGCCTTTAACTGTCAAACTGTC6890.14807014527636894No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6810.14635089830654174No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCTCTG6810.14635089830654174No Hit
GTTTCCTCTGGTCCTGGTATGAACAATGTATTTACCCAAAAGTGAAACAT6740.14484655720794293No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG6200.1332416401616092No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6120.131522393191782No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5730.12314106421387433No Hit
TCCAGATGATTCTTTAACAGGTAGCGTAGCAGAGACTGGGTCGTAGCCAT5550.1192727585317631No Hit
CTTGGGCCTGTGTTATCTCCACTTTGTCTGGTCTTTGCCTGCTTGCCTCT5440.11690879394825066No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG5350.11497464110719505No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT5330.11454482936473825No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT4810.10336972406086134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)