FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419459
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA298277.110826087889401No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA236535.6389301457353405No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC196704.689373693257267No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT171864.097182322944555No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC124622.9709697491292353No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT113052.6951382614272195No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG104732.4967875286976797No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC102502.4436238106704113No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA101922.4297964759368615No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG87292.0810138773992213No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG83441.9892289830472107No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA82531.9675343716549176No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC78481.8709814308430623No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT77331.8435651636989552No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG69941.6673858470076932No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC64421.5357877647159794No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG57011.359131643378733No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA53311.270922783871606No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG51151.2194278821052833No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA50191.1965412590980287No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC47011.1207293203864979No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG46111.0992731113171967No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG46001.0966506857642822No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT44641.064227969837338No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC41110.9800719498210789No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA39440.9402587618813758No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC38920.9278618410857795No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC37560.8954391251588355No Hit
TCTTAAATGGCTACGACCCAGTACACTGACGACATGGTTCTACATCTTAA34180.8148591399874601No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT33750.8046078400987939No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTAAGTCATGAT33590.8007934029309182No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG33370.795548551825089No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCTTTTACT32610.7774299752776791No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG29740.7090085085789076No Hit
TCCCTGCCAGCGAGATGTACACTGAGGCCTTTGCATCCCTAGACCAAGTC28620.6823074484037772No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA26210.6248524885626485No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGGC23090.5504709637890711No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA23020.5488021475281255No Hit
CAGCCTCTGGCATTCTGGCCTGGCCCTCATGCACAAGAGCAGTCAGAGGA21570.5142338106942513No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA20230.48228789941329187No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19370.46178529963595966No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA18620.443905125411542No Hit
CTGGTGTTGTTGGGCAGTGTCCAAATCTCCCCTTTCTTGCGGAGATAGAC18140.4324618139079147No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA17520.4176808698823961No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16060.3828741307255298No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC15610.3721460261908792No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCAGGTGCCGGGC14800.35283543802850814No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT14510.34592177066173335No Hit
GGTCTTCTCTGTTTCAGGGCATCTTATCCGAGTGGAGGTGGCTTTAGGTC13690.32637278017637No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC13430.3201743197785719No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTA10170.24245516248310325No Hit
GGGGTCAGAGGCAAGCAGAGCCAGACTGGGAGATAACACAGGCCCAAGAG10000.23840232299223524No Hit
GGGGTCAGAGGCAAGCAGTCAGTGGGAGATAACACAGGCCCAAGAGACCA9310.221952562705771No Hit
ATACGGCCAGGCATTGAAGTATACTGACGACAGGGGCCAGGAGGAGACCA9050.2157541023079729No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGACGCGGACGCGGGTGCCGGGC8820.21027084887915146No Hit
GGTCTTCTCTGTTTCAGGGCATCCCCAGGAGGTGGCTTTAGGTCAGCAGA8810.21003244655615924No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC8080.19262907697772608No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC7770.18523860496496677No Hit
GGTCTTCTCTGTTTCAGGGCATGTTTAGAGCAGAGGAGGTGGCTTTAGGT7210.1718880748774016No Hit
ATACGGCCAGGCATTGAAGTCAGAGCAGTCAGAGGACCAGGAGACCAAGT7200.17164967255440935No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA6760.16115997034275104No Hit
ATACGGCCAGGCATTGAAGTAGGAGACAGGGGCCAGGAGGAGACCAAGTC6410.1528158890380228No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT6280.14971665883912372No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6100.1454254170252635No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC6060.14447180773329457No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC6030.14375660076431784No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC5630.13422050784462844No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA5400.12873725441580702No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGCAGCTCGTGGTGA4880.11634033362021079No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT4660.11109548251438163No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG4230.1008441826257155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)