FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419459
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC254376.0642398899534875No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA227335.419600008582483No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA183944.385172329119174No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC115412.7514012096533866No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC110752.640305727139005No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG102552.4448158222853724No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA102452.4424317990554503No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA101902.429319671290877No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG101322.4154923365573273No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT92622.2080823155540825No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG85192.0309493895708517No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT82771.973256027406731No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT75191.7925470665786167No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA64471.5369797763309405No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT56361.343635492384238No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTATAGTCTCCCTTGCCGTC54011.2876109464810626No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA52761.257810656107033No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT50101.1943956381910985No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG47271.1269277807842961No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT45971.0959354787953055No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT45261.0790089138628567No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC44721.066135188421276No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC44621.0637511651913536No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA44171.053023060656703No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA43761.0432485654140213No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA42591.0153554936239297No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT40490.9652910057955605No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT39630.9447884060182283No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC37500.8940087112208821No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA37100.8844726183011927No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC36750.8761285369964644No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG33960.8096142888816309No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT33630.8017470122228871No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC32740.7805292054765781No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA30990.7388087989529369No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG29590.7054324737340241No Hit
AGGGATGCAAAGGCCTCAGTGTACATCTCGCTGGCAGGGATGTAGAACCA28470.6787314135588937No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT25720.613170774736029No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACTAGGGC25310.6033962794933474No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT25050.5971978190955493No Hit
CCTGGTCCTCTGACTGCTCTTGTGCATGAGGGCCAGGCCAGAATGCCAGA21260.5068433386814921No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA19310.4603548856980062No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA18890.4503419881323324No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT18520.44152110218161966No Hit
ATCTCCGCAAGAAAGGGGAGATTTGGACACTGCCCAACAACACCAGTGTA18100.4315082046159458No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA17350.41362803039152807No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG15950.3802517051726152No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG14810.35307384035150036No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG13980.33328644754314485No Hit
GCTGACCTAAAGCCACCTCCACTCGGATAAGATGCCCTGAAACAGAGAAG13460.32088952674754867No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC12920.3080158013059679No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC12330.29395006424942605No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTTCTACAGTACTCCCCTG11500.27416267144107054No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT10210.2434087717750722No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA10100.24078634622215758No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10090.24054794389916537No Hit
CTTGGGCCTGTGTTATCTCCCAGTCTGGCTCTGCTTGCCTCTGACCCCTG9860.23506469047034395No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT9280.22123735573679432No Hit
CTTGGGCCTGTGTTATCTCCCACTGACTGCTTGCCTCTGACCCCTGTAGA9240.22028374644482537No Hit
CCTCCTGGCCCCTGTCGTCAGTATACTTCAATGCCTGGCCGTATTGTAGA9050.2157541023079729No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT8880.21170126281710488No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA8720.2078868256492291No Hit
GCTGACCTAAAGCCACCTCCTGGGGATGCCCTGAAACAGAGAAGACCTGT8720.2078868256492291No Hit
GTTTCCTCTGGTCCTGGTATGAACAATGTATTTACCCAAAAGTGAAACAT7870.18762262819488912No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA7760.18500020264197453No Hit
CCTGGTCCTCTGACTGCTCTGACTTCAATGCCTGGCCGTATTGTAGAACC7190.17141127023141714No Hit
GCTGACCTAAAGCCACCTCCTCTGCTCTAAACATGCCCTGAAACAGAGAA7160.1706960632624404No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG6650.15853754478983645No Hit
CCTCCTGGCCCCTGTCTCCTACTTCAATGCCTGGCCGTATTGTAGAACCA6490.15472310762196068No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG5930.14137257753439547No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT5800.13827334733549643No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT5790.13803494501250418No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA5600.13350530087565174No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGGGAGAGACAGAGATTTCA5490.13088287532273715No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC5370.12802204744683032No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG5220.12444601260194678No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGAGAGATTTCA4930.11753234523517198No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC4480.10680424070052137No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA4200.1001289756567388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)