FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792442
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT204262.57760189389255No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC158922.005446455387271No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT145981.8421537475297876No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG145491.8359703296897438No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT123701.56099752410902No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA123331.5563284126787829No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC117291.4801083233851815No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT117211.4790987857786437No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA111851.4114597661406134No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC105711.3339777548388398No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT102401.29220813636834No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT100291.2655815819959064No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA99891.2605338939632176No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG94321.1902448381080255No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG93681.1821685372557236No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC92051.1615992085225164No Hit
GAATCAGGGATCACAACACCCCCCAAAATGAGGAGAGTAGCAGAAACTGA91881.1594539411086238No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG90681.1443108770105572No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC89531.129798773916577No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA85771.0823505064093017No Hit
TTCCATGAAGCAAACGCTGATGAATGTGAAAAATCTAAAAACCAAGTGAA85221.075409935364355No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA80571.0167305619843472No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA79511.0033541886977217No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA78180.9865706259890314No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT72900.9199411439575389No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT68970.8703476090363711No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT68510.864542767798779No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA66650.8410710184467759No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG66220.8356447538116355No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC65660.8285779905658711No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG64930.819365959906214No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG64750.817094500291504No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT64550.8145706562751596No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC60650.7653556979564435No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA59030.7449125614240538No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA57730.7285075753178151No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA57160.7213146198712335No Hit
GCTGGCTTGGGATGGACTTTTTAAAAAGACACTCAGGTTTGGAATGTGGA57010.7194217368589751No Hit
TGAAGTGGACTGTTTATCTTCTTCACTTACTAGAGACAATTCAATGTCTG55040.6945618732979827No Hit
GCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAGA54060.682195037617895No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT53630.6767687729827546No Hit
ATTTAACACCTAGCCAAAAGGCAGAAATTACAGAACTTTCTACTATATTA52260.6594804414707953No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC52140.6579661350609887No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT51620.6514041406184932No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC51350.6479969511964283No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTAATCCACTTTGAAAAAGAAAACA50300.6347467701106201No Hit
ATCCTGTTCCAAGTCAAGACCACATTTAGCTGGGACACATACTTCTCTTA49580.6256609316517803No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT49450.6240204330411564No Hit
TCCTGGCATGTGTTTCTACAGAGCTGATTTTCTTTAAAAGTGAATGACTC49440.6238942408403391No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT49330.6225061266313497No Hit
TGTTTTTGTAGGTAATTTTAACCAATCAGATGACAACAAAGATTGATAGA48630.6136726725741443No Hit
TCCTCCCCTCTTAGCTGTCTTACCAACCCTCCTAAACCACTGAAAAAAGA47170.5952486112548301No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC46820.5908318842262273No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA46190.5828817755747424No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA42870.5409859649034251No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG42830.5404811961001562No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA41950.5293762824282409No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC41860.5282405526208858No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG40540.5115831821130127No Hit
ACCAAAGAAGTCCACGAATGGTTCCTGATGGAATCAACCCAAAATTACAC39860.5030021124574416No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT39460.49795442442475285No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA38490.48571378094548245No Hit
TGGACATTCTAAGTTATGAGGAAACAGACATAGTTAAACACAAAATACTG37110.46829925723270605No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA36920.4659016054171788No Hit
GGGATCGATGAAGGACCGGAACAGCCGCGTGGGAGGAGGGCAGTACACAG36870.4652706444130927No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT36490.4604753407820383No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC35980.45403953854036005No Hit
ATTCATGGGTTGCAAGTAGAAGTTTGTCCTCTTAATGGCTGTGATTACAT35450.44735135189704733No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA35090.4428084326676274No Hit
GTGCAGGCTGATTTTCTTTTTCCTGTGTATCTTCTACCAGGTGCTTGGGC34400.4341011708112392No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA34190.4314511345940775No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC33820.4267820231638404No Hit
AAAGGGTCCCACTGCTACTAACTAGCCTCCTCTTTGTCAGGCCAAGCACA33630.4243843713483132No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA32850.41454137968456994No Hit
CAACTTGTATTCAGCAGGTACCGCACTTCTTGAACAAAACCTGCATACTT32430.4092413072502467No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTA32010.4039412348159234No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT31750.40066023759467567No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA31480.3972530481726107No Hit
CTTTTTCTGGTTGGGCAGTTGGTGGAATTAATACACTGTCTTCATTAATT31360.3957387417628041No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG31240.39422443535299745No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC30300.3823623684761787No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC29620.3737812988206077No Hit
TGCCAAAATGACGAACACAAAGGAAAAGAGAAAAGGCATCTGTATATGTT29250.36911218739037055No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA28800.3634335383535956No Hit
GCCCTGCACAGAATTTAGTTGGAGAGAACAATCATGATGTTGATAACAGT28290.3569977361119173No Hit
AGCAGGATTTTTGCTACTGATTTCTTCCTGTTCCTTTAGTCTTTTCCCAG27950.35270720128413185No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA27220.3434951706244747No Hit
AGGTGTGTGTGTGGCTTCAAAAACATTGTTTGCTGTTTCTCTTTTCTCCA27030.3410975188089475No Hit
CAAAGTGGGAAGGGGAGTGAATAGAGGCCCATCCCTGATCCTTTGACTAA26380.3328950257558282No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC26100.329361644132946No Hit
ACCCGAACGTGATGAAAAGATCAAAGAACCTACTCTGTTGGGTTTTCATA25940.32734256891987046No Hit
TCAGTCTCATCTGCAAATACTTGTGGGATTTTTAGCACAGCAAGTGGAAA25090.3166162318504067No Hit
CAAAGAGACTCACCTAATTTTCTTACATTTTGTTTTTGTAGGTAATTTTA24960.31497573323978284No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC24790.31283046582589014No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG24590.3103066218095457No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT24330.3070256245882979No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTAAAA24240.305889894780943No Hit
ACCTGAATGTGACCAGTGTTTTCCACTTTTTCAGGATGGTAGTGCTTTGG23390.2951635577114792No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC22910.2891063320722526No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC22400.28267052983057434No Hit
TCTGGGGTATCAGGTAGGTGTCGTCAGTGTGGCTCTAATCAATCGACTCC22080.2786323794044233No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTACTAA21830.2754775743839928No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTTCTTGCTTTTAATTT21600.2725751537651967No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC21160.26702269692923897No Hit
TTGTCAGATTTAACTTTTTTGGAAGTTGCGAAAGCTCAAGAAGCATGTCA21150.26689650472842175No Hit
CTCCTAATTGTGAGATATATTATCAAAGTCCTTTATCACTTTGTATGGCC21040.26550839051943237No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC21000.2650036217161635No Hit
AGGAGACACATCAAGGATGTTTAGGAGAACAAAGAGCTATGACAGCCTGC20640.2604607024867435No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG20140.25415109244588246No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG19930.2515010562287208No Hit
GTTAACTGCTACTAAAACGGAGCAAAATATAAAAGATTTTGAGACTTCTG19710.248724827810742No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC19520.2463271759952148No Hit
GATTTTATGAGACAACAGAAGCATTATACTGCTTTTTTGATGCATAAAGC19150.24165806456497765No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACGCGATATATGT18910.23862945174536435No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG18310.23105791969633108No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC18240.23017457429061056No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTAC18190.22954361328652445No Hit
TTTTTAGGAAGTACATCTCAGAATCTTGATTCTGGAACAGATTTGTCTTT17490.22071015922931897No Hit
ACTGCAGTTTTCTGTCGTGAACGTTTCTTATTTTTTGGTAGAACCAACAA17420.21982681382359845No Hit
CCTCTGAAAGTGGACTGGAAATACATACTGTTTGCTCACAGAAGGAGGAC17320.21856489181542624No Hit
CCAAAATATGTCTGGATTGGAGAACGGTAGCACAGCAAGTGGAAAATCTG17280.21806012301215735No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT17270.21793393081134013No Hit
TGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTG17240.2175553542088885No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA16350.20632424833615584No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC16220.20468374972553194No Hit
ATATTTAGTGAATGTGATTGATGGTACTTTAATTTTGTCACTTTGTGTTT16150.20380040431981142No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT15720.19837413968467094No Hit
CTGCACACTGACTCACACATTTATTTGGTTCTGTTTTTGCCTTCCCTAGA15650.19749079427895036No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC15220.1920645296438099No Hit
GCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAAA15100.19055022323400325No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT15070.1901716466315516No Hit
ATCCTGAGTCTTATGTCTGATACCATGTTTTTGTTTTGTTTTTAGAGTCT14700.18550253520131443No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA14590.18411442099232497No Hit
TATTTGTTCAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGCTTAT13760.17364046832449567No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC13070.16493320646810744No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA12800.1615260170460425No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA12610.1591283652305153No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA12600.15900217302969807No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC12320.1554687914068159No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATGTAAGTCCAAAAGG12220.1542068693986437No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTCTTGCTTTTAATTTT12050.15206160198475094No Hit
ATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTT11930.15054729557494428No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTTTGTTCACATTCAAAAGTGACTT11620.14663533734961043No Hit
TGGAAGAAATGTTGGATAAAGCATAATTTGTCAAGTCTCAACTAATTAAG11450.1444900699357177No Hit
ATCATGATAAAACTCTTCAGACTGATTCTATAGACAGGTATTGCACATGG11430.14423768553408325No Hit
GGGCAGTTGGCCACTTTTACTTATAGCTTTATTTACAAGGAGGTTATCTG11300.14259718692345938No Hit
TCAACTTGAGGGAGGGAGCTTTACCTTTCTGTCCTGGGATTCTCTTGCTC11270.14221861032100772No Hit
CACCATTTATCTGAGGACTGGATACGGTAGCAGAAGCCTTTTTAGCCCTA11060.1395685741038461No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCTCCCGACATATGT11010.13893761309976No Hit
AGGGCACCGTTTGCTTAACAAAATCTCCACTGGTGCTCAGAATGCTGGTT10880.13729711448913612No Hit
GGAAGTTAGAAGTTTGTGACATTTTATTTACTGTATTACAAAAAATCACT10630.1341423094687056No Hit
ACACCACTCTCTAAATCAAACACATTGAACTCATTTTCTAAGATAAGAAA10520.13275419525971618No Hit
GACTACAGATGTGCCACCATACCCAGCTAATTTTTTAATTTTTTGTAGAG10510.13262800305889894No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT10380.1309875044482751No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG10100.1274541228253929No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA9820.12392074120251072No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCGGATATATGT9580.12089212838289742No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTAC9530.12026116737881132No Hit
TCTGGGGTATCAGGTAGGTGTCGTCAGTGCAGCAACAGAGGAGGGGGAGA9520.1201349751779941No Hit
TGCATTGAACAGATGAAACTAAAGAACTTTTTTTTTCTTAAGGTTGTAAG9410.11874686096900466No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA9320.11761113116164967No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC9280.1171063623583808No Hit
GAATGAGGCAAAGTAGCCTAAAGAAAGATTGGTTCTTATCAGAAGAAGAA9030.11395155733795027No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC9020.11382536513713307No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGAAGGTAATTA8560.10802052389954091No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCTGAAGATTCTCCAGATATTTTG8530.10764194729708926No Hit
GATGTCAGATACCACAGCATCTTTACATTGATGTTTCTTACCTTTCCACT8510.1073895628954548No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTACTA7980.10070137625214212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)