FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792442
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT193192.4379071275878865No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG170872.15624613536385No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT147451.860704001049919No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT144881.8282726054398932No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC120751.52377082486794No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA118701.4979014237004096No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG116671.4722844069345138No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT112971.4255932926321422No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT111161.402752504284225No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA104891.323629994371828No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC102031.2875390249381027No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC99401.2543504761231736No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA97421.2293644203613638No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC95301.2026116737881132No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC90281.1392631889778684No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA89901.134467885346814No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG89141.1248772780847052No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA88101.1117532891997142No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC87141.0996388379212612No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA83511.05383106902461No Hit
AAGACGGTACAACTTCCTTGGAGATTTTGTCACTTCCACTCTCAAAGGGC82151.036668929713468No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT79861.0077709157263244No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT79120.9984326928658501No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG77060.9724370994975027No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT70600.890916937769578No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA69750.8801906007001142No Hit
TTCTGACTTTCAGTAAGGCAATGAAAAATAATACTCTTACCAGCTACTTG66160.8348876006067322No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG65720.8293351437707744No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT64290.8112896590539119No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA63670.8034657426032441No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA63420.8003109375828136No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG63300.7987966311730069No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA61170.7719176923989389No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG60860.7680057341736052No Hit
GGAAAAACCAAGTGTGGGAGGCCCCAGTCCTCTCTGCCAATCCGCTATAC57240.7223241574777712No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA56260.7099573217976836No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG55740.7033953273551882No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC55300.6978428705192304No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT53040.6693234331345386No Hit
ACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTATTTCATCATTA53000.6688186643312697No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA51510.6500160264095038No Hit
CATCTGGTTTTCAGGCACTTCAAATGTACTCTTCTGCAATATGTAGCTTG51430.6490064888029661No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT50300.6347467701106201No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT49280.6218751656272636No Hit
CTCAGTCTGTCTTGCCAGTGATACTAAACAATTCGACAGTTCAGGCAGCC48980.618089399602747No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG48600.6132940959716926No Hit
TCACAGCCATTAAGAGGACAAACTTCTACTTGCAACCCATGAATTGCTCT47980.6054701795210249No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC47620.6009272602916049No Hit
AACTTATTTGTTTTCTTTTTCAAAGTGGATTACGGTAGCAAAAGCGTCCA47550.6000439148858844No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGATAAACTAAAATGTTTATCT47370.5977724552711744No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA47250.5962581488613677No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA46390.5854056195910868No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG44230.5581481042145672No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA42320.5340453938584779No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT42070.5308905888380474No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA41360.5219309425800247No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT40210.5074188394860444No Hit
TTTTTCAGTGGTTTAGGAGGGTTGGTAAGACAGCTAAGAGGGGAGGATGT40110.5061569174778722No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG40070.5056521486746033No Hit
CGACCTGCTAAGTAAGAACAGGGGAAAAATGCATGTGTCAAATGCAAAAT38920.49114004558062296No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACGATATCACC38410.4847042433389447No Hit
GTGTACTGCCCTCCTCCCACGCGGCTGTTCCGGTCCTTCATCGATCCCTG36590.4617372627902105No Hit
TATGGTCCTCAGAAAAGTGCACCAAACCAGATTCTAGCAGTCCCATTAAG36410.45946580317550056No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG36350.45870864997059724No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC36290.45795149676569386No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT35470.44760373629868183No Hit
CCGCTAGCTGTATGAAAACCCAACAGAGTAGGTTCTTTGATCTTTTCATC34840.4396536276471969No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT34770.43877028224147635No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC33950.42842252177446427No Hit
CTGTCCTTTTCCACAATCACACATATTCTTTCAAACATACTCTGCAGGTG33790.42640344656138873No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA33640.4245105635491304No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT33030.41681283929928No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA32180.4060865022298162No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG31790.4011650063979446No Hit
AATAGCAACAAGAAAAGATGGAAGAGTACTAAATTTGAGTAATCTAGGAA31730.40040785319304123No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC31300.3949815885579007No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT31020.3914482069350186No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG30910.39006009272602915No Hit
TGGGAAAAGACTAAAGGAACAGGAAGAAATCAGTAGCAAAAATCCTGCTA29600.37352891441897323No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG29530.3726455690132527No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA29210.36860741858710167No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT28740.36267638514869227No Hit
ACCCAAGAACATTTTCCCCACAGGGTCAGTGACCCTAGTGGTGAGCAAAA27240.34374755502610915No Hit
AGGCGTTTCACTAATAGCAGTTTTGTCTGACACAGGTAAAGATTCGCTTT26740.33743794498524815No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC26110.3294878363337632No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG26100.329361644132946No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT26100.329361644132946No Hit
ATGGGCAGCACTGAGCATAAGCTTAAAGAAATGGCTTAATCCAAGGATCT25830.3259544547108811No Hit
TATTAAAAGGTTACTCCTCACATCACCCCATTTTTCCTTATATTTGGCTT25180.3177519616577617No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTATGTCTACAATATCGTCT25030.3158590786455034No Hit
ACTTTAGGGTCTTTGCCCATTGATGGCTAAAACTGGTGATTTCACTAGTA24880.31396619563324507No Hit
GTAAAACCCACTAATACTTGAAGGTCATTCTTTTTTTACCTTGTAAGACA24500.3091708920021907No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG24310.3067732401866635No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGACTTGGTCTGTCTAAGAACAC24170.3050065493752224No Hit
TGTGAGCTGGTCTGAATGTTCGTTACTTTTAAACAATACTTTGGAAAAGA24130.30450178057195354No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC23540.2970564407237375No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG23260.29352305910085535No Hit
CCAGAAGTGATTTCATGACCACCTACAAGAATACAGGTTCCTTTTCCTTC22700.28645629585509097No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT22340.281913376625671No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG22100.2788847638060577No Hit
CCTGGAGTCGATTGATTAGAGCCACACTGACGACACCTACCTGATACCCC21920.2766133041913478No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC21730.2742156523758206No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG21150.26689650472842175No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC20770.26210120109736734No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA20170.25452966904833413No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT19900.2511224796262692No Hit
GGCGACACTAATATTTTTCCCACTTGCAGTCTGAAAAAATGTATCAGAAG19780.24960817321646253No Hit
ACCACTGTTGGAACATGAATACCAACATTCTTTCTTTGTTCCACTAAAGA19670.24822005900747313No Hit
AAGGAAAAGTTATGCAATTCTTCTGGTTTCTGATCAAAGAAATTTACAAT19390.2446866773845909No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG19310.24367713977805316No Hit
CTGGAGTGTGTGGAGGAATTACATTCACCTCTTCATCAAGGTTACTTTTT18930.23888183614699876No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTACCGTC18420.2324460339053205No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT18220.2299221898889761No Hit
CATAGGTTTTAAAAGTTTTGCCACTTAATTAGCACTTTCTTTGCATCTAG17350.21894346841787787No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT17320.21856489181542624No Hit
GGGATTCCATGATTCGATGTTCACATCGTTCTAAATGTTTTATCATTTCT17090.21566247119663018No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT17040.21503151019254407No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT16980.21427435698764072No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG16800.21200289737293077No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTTCTCCAATCCAGACATATTTTG16730.21111955196721024No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG16360.20645044053697303No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC16090.2030432511149081No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT15540.19610268006996098No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG15410.1944621814593371No Hit
GCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAAC15320.1933264516519821No Hit
AACTTATTTGTTTTCTTTTTCAAAGTGGATATTAAACCTGCATTCTTCAA14840.1872692260127555No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG14720.18575491960294888No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA14200.1791929251604534No Hit
AGCAAACTTTCATCTTGATCAGTGGACAATACTGGGAGGTCACTGTTATC13340.1683403958901724No Hit
AGTTGAAGAAGCACCCTTTCTGGGCTTAGGCATCTATTAGCAAATTCCTT13280.16758324268526908No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG13140.165816551873828No Hit
AGAAATCCAAGGCTCTTCTCTTTTTGCAGTTCTTTTGGTCATCAATCTCT13100.1653117830705591No Hit
TCCCCTGGAAACTGCACTGACCCTGTGCCCTGGTAACTGCTGGACTCCCC12650.15963313403378418No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT12560.1584974042264292No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA12460.15723548221825698No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA12300.15521640700518147No Hit
AGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCG11650.1470139139520621No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTAAAGACTATATAATCTACC11370.14348053232917993No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTATCCAGTCCTCAGATAAATGGTGT11080.1398209585054805No Hit
ACTGTAATAAATGGAAAAACATTTTACTTACCTTTATATCACTACCTGGT11030.1391899975013944No Hit
AGTCATGTTGATCCATTCCTGTAGGGAGTCTGTGTCAGACAAATCTATGG10960.1383066520956739No Hit
ATATGACGTGTCTGCTCCACTTCCATTGAAGGAAGCTTCTCTTTCTCTTA10850.13691853788668445No Hit
TCCTATTTCATACATCTAAATTCAGAACCTTTTTGAAAACCAAGAGTGCA10610.13388992506707115No Hit
AGCGGTAGTAGTCAGCACCTTGAACACATTCCTCCTAAAGGTAGCAGTGA10540.1330065796613506No Hit
CCAGACACTAAGAGCAGGAATGAAGTAGTGACTCCTGAGAAGGTCTGCAA10510.13262800305889894No Hit
ACATTGGACCACTGCTTTTAGACTTTTCTAGAGAAAATTCTGAATAATCA10490.1323756186572645No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC10390.13111369664909228No Hit
CAGCCATATCAGTCTGTGGGATCTGGTACAGTTTTGTAAATTCATCTGGA10120.12770650722702734No Hit
TCATTGGAATGGCAAAGTCTCATACTTGTAATCCTAGCGCTTTGGGAGGC10090.12732793062457567No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG10010.1263183930180379No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT9660.12190166598943519No Hit
CCCCCTCCTCTGTTGCTGCACTGACGACACCTACCTGATACCCCAGATGT9580.12089212838289742No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT9500.11988259077635967No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCTTTGATGTCTACAATATCACC9140.11533967154693972No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG9090.11470871054285361No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG9020.11382536513713307No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA8900.11231105872732641No Hit
ATATTCAACTTACTGGTTTGTTTTCCTCATTTTGTAAAGAAGAAAACTGA8860.11180628992405754No Hit
TCCTTCTTTTTCCTACTGAGTTCAGCTCCCAGACTGGAATTAAGTGGAAG8750.1104181757150681No Hit
AAACACAGAAGGAATCGTCATCTATAAAACTATATGTACTATTTACAAAA8730.11016579131343367No Hit
GTTTTCTCATTCCATTTAAAGCAGTATTAACTTCACAGAAAAATAGTGAA8490.10713717849382036No Hit
TTCAATTACCAGTTTATTTATGAAATCCCTAAATGTCATAAAAATGGTTT8280.10448714227665873No Hit
CCATGGACAAGAGAAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGT8230.10385618127257262No Hit
TGCACGTACCACATTTAATTACCTTCAGAGACTTGGTCTTCTCTTGGTAT8050.10158472165786266No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT8050.10158472165786266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)