FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312117
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC213576.842626322821249No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC208066.666089959854799No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT176475.653969505025359No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC167775.375227879288856No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA140504.501517059307888No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC96463.090507726269316No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA89342.862388142907948No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC82572.6454823031106924No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC78602.518286411826334No Hit
TGGTGATAGATGACTTGCTGCTACCGTAAGCCTGCAGTGATATTAACTGT76482.4503631650951405No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAAGT71432.2885648650986647No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA58231.8656465363950059No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT55071.7644024516447359No Hit
GGGAAGCTCTTCATCCTCACTACAAAACTAGTATCTTCCTTTATTTCACC54771.7547906714469252No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAAGTG52961.6967995975868024No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA44491.4254270033352878No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT42261.35397943719823No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAAGTGGTG36991.1851324983900269No Hit
GATTCCCGCGCCCAGAGGTACACTGGCGACATGGTTCTACAGAGTCCCAC36931.1832101423504648No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG36451.1678312940339681No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA34311.0992672619562536No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAAGT32281.0342275492844029No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC31471.0082757427503148No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG31401.0060329940374924No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG30000.9611780197810437No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAAGTGG28380.9092744067128672No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT27520.8817206368124773No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTACAGCAAAAAGTCCTGCAACT27130.8692253225553238No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT26310.8429531233479753No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA26270.8416715526549339No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAG25850.8282150603779993No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAAGTG23510.7532431748350779No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAAGTGGT22390.7173591954299189No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA21890.7013395617669015No Hit
TGGTGATAGATGACTTGCTGCTACCGTACAAAGGTTGAATGGTGCTGAAG21090.6757081479060737No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG21040.6741061845397719No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAG20650.6616108702826183No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC19800.6343774930554889No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT19730.6321347443426664No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAAGTGG17820.5709397437499399No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAAGTGGTGATCATC17550.5622891415719106No Hit
TACTGAATGCAAAGGACACCACATGACGACATGGTTCTACAGATTCCCGC17150.5494734346414967No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAAGTGGTGA16350.5238420207806688No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC16210.5193565233550239No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT15450.4950066801872374No Hit
CACCTCGAACACCCAGGCAATCTGAATTGTTAGCCAATCAGACCAAGTCT14380.46072466414838026No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG13900.4453458158318836No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAAGTGGT13520.4331708942479903No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAAGTGGT11180.35819900870506893No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAAGTGG11000.35243194058638266No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG10040.3216742439533893No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA9970.31943149524056685No Hit
CTACAGTTATTTATTACCCCAGAAGCAAAGAAATAATACCCATGACAGGA9350.2995671494984253No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT9210.2950816520727804No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTAAAATG8820.28258633781562686No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAG8820.28258633781562686No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC8650.27713966237020093No Hit
CCTCTCTCCTTCTTCCCCAAGTACACTGACGACAGGGGCCAGGAGGAGAC8300.26592591880608873No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG8140.26079963603392314No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG8130.26047924336066286No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA7150.22908076138114875No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTAAGTGGTG6440.20633288157966403No Hit
TCTTGCAAAAACATCCACTCTGCTACCGTAAGTGCAGGAATGGATGAATG6240.1999250281144571No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT5430.1739732215803689No Hit
GGCTTCCACTTAATTCCAGTCTGGGTGCGGAAGAGAAAGACCAAGTCTCT5270.16884693880820334No Hit
GGATCCTGGGTGTTTGTATTTGCTACACTGACGACATGGTTCTAAATTCT5260.16852654613494297No Hit
TGTACTTGAAAAAGGACTTTAGAGCAAGTCTCTGCTACCGTATACCTGTT5240.1678857607884223No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC5200.1666041900953809No Hit
AGTCTTCAGAATGCCAGAGATACACTGACGACATGGTTCCCTCTGCGTGT5200.1666041900953809No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTCCTCAGA4810.15410887583822733No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAAG4710.15090494910562385No Hit
GGTCACTATTTGTTGTAAGTATTTTTGTTACACTGACGACAGGATAATAG4570.146419451679979No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTAAGTGGTGATCAT4570.146419451679979No Hit
GGAATCCTATGGCTTTCCAACCTACGGTAGCAGAAAGTCAGTCCCATGGA4440.14225434692759445No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA4290.13744845682868925No Hit
TCAGCCTTAGCTTTTTACACAAGTACACTGAGTGCATGTGCCAGACATCC4280.13712806415542889No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTAAGTGGTGATCAT4260.13648727880890818No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAAGTGG4020.12879785465065985No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT4010.1284774619773995No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA3530.11309861366090279No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA3480.11149665029460105No Hit
GCCCTCTCAGAAACATCTGCTACCGTACTCATGGTGGGGGCAGCAGACCA3310.10604997484917515No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTAAG3270.10476840415613375No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA3260.10444801148287342No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC3240.10380722613635271No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA3210.10284604811657168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)