FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312117
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA211716.783033285594825No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG206116.60361338856903No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC174305.584444294927864No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG159955.124680808799265No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT139544.470759362674894No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC95483.0591092442898016No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT88742.843164582512327No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG82142.6317054181604975No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG77732.490412249252684No Hit
AGACAGTTAATATCACTGCAGGCTTACGGTAGCAGCAAGTCATCTATCAC73192.344953975592486No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA71382.286962901732363No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC56331.8047719284755397No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT54521.7467808546154164No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGTAGTGAGGATGAAGAGCTTCCC54461.7448584985758546No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT52571.684304283329649No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT44261.4180579718502997No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT42361.3571833639308335No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC36761.1777634669050387No Hit
GAGGGGGTCGTGGGACTCTGTAGAACCATGTCGCCAGTGTACCTCTGGGC36491.1691128647270093No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC36161.158539906509418No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA33951.0877331257188811No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA32111.028780873838977No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG31391.005712601364232No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT30190.9672654805729902No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT29800.9547701663158368No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC28240.9047889092872223No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT26100.836224877209508No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT25990.8327005578036442No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT25900.829817023744301No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG25790.8262927043384373No Hit
ACAAGTTGCAGGACTTTTTGCTGTACGGTAGCAGAGCTTAGGTGGCACCA25000.8009816831508696No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT23480.7522819968152968No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT22260.7131940906775344No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT21850.7000579910738601No Hit
TCAGCACCATTCAACCTTTGTACGGTAGCAGCAAGTCATCTATCACCATG21030.6737857918665116No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG21010.6731450065199909No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG20600.6600089069163166No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT19540.6260472835507198No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA19530.6257268908774595No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT17820.5709397437499399No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17490.5603667855323484No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCATGTGGTGTCCTTTGCAT17460.5594056075125674No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG16240.5203177013748049No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG16080.5151914186026394No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG15380.492763931474415No Hit
GATTGGCTAACAATTCAGATTGCCTGGGTGTTCGAGGTGTGTAGAACCAT14320.45880230810881817No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC13690.43861756969341625No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT13460.43124853820842823No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT11180.35819900870506893No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC10880.3485872285072585No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC10010.3207130659336082No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC9940.3184703172207858No Hit
CCTGTCATGGGTATTATTTCTTTGCTTCTGGGGTAATAAATAACTGTAGT9200.29476125939952No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT9080.29091654732039585No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG8740.28002319642954404No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTACTTGGTCTGTCTAAGAACACAG8630.2764988770236802No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG8600.2755376990038992No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTGGGGAAGAAGGAGAGAGGTGTA8160.2614404213804439No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT8020.256954923954799No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT7960.25503256791523693No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC6470.2072940595994451No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT6320.20248816950053988No Hit
TTCATTCATCCATTCCTGCACTTACGGTAGCAGAGTGGATGTTTTTGCAA6000.19223560395620873No Hit
GTCTAAGAACACAGAGGAGAATTTAGAACCATGTCGTCAGTGTAGCAAAT5300.16980811682798438No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC5230.16756536811516196No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA5180.1659634047488602No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT5170.16564301207559984No Hit
TATGAGAACACGCAGAGGGAACCATGTCGTCAGTGTATCTCTGGCATTCT5140.16468183405581882No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTGCAGTTCTTTT4760.1525069124719256No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4690.15026416375910315No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG4660.14930298573932213No Hit
CCATGGGACTGACTTTCTGCTACCGTAGGTTGGAAAGCCATAGGATTCCT4410.14129316890781343No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4300.1377688495019496No Hit
ATTAGGATGTCTGGCACATGCACTCAGTGTACTTGTGTAAAAAGCTAAGG4220.13520570811586682No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT4210.13488531544260646No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG4030.12911824732392022No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC3940.12623471326457708No Hit
AGGATTTTTCTTGATTTTCTATTATCCTGTCGTCAGTGTAACAAAAATAC3840.1230307865319736No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT3510.11245782831438211No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3300.10572958217591479No Hit
GCTGCCCCCACCATGAGTACGGTAGCAGATGTTTCTGAGAGGGCTGTAGA3290.10540918950265445No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG3270.10476840415613375No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT3220.10316644078983202No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA3180.10188487009679062No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGTGTAGAACCATGTCGTC3170.10156447742353028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)