FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348616
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC240586.9010028225898985No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC200195.742421460862381No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT191875.503763453197788No Hit
TTTTAGGTGCTTTTGAATTGTGGAACAGTTTTCTTAACCATATCTAGAAG162164.651536360924341No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG119063.415219037565689No Hit
TCAGATATAAAAGAAGAGGTCTTGGCTACCGTACAAACCTAGTCATGATT98002.811115955664685No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC96322.7629253964247193No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA86362.477224223787778No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC85642.45657112697065No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC80182.29995180944076No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC79592.283027743993391No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAACT70402.0194139110081006No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG70342.0176928196066735No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT59631.71047800445189No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCAAGGTAATT58601.68093260206072No Hit
AGAAGACCAGGAAAGAACGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC53741.541524198545104No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAACTG53271.5280423159005898No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC45711.3111847993207426No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA45021.291392248204328No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC44611.279631456961241No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA41561.1921426440553502No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA40421.1594419074282305No Hit
GGGAAGCTCTTCATCCTCACTACAAAACTAGTATCTTCCTTTATTTCACC40291.1557128760584712No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAACTGTAG39161.123298987998256No Hit
TCTGGGGTATCAGGTAGGTGTCTACGGTAGCAGAAAGTCAGTCCCATGGA38521.1049406797163641No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT36681.0521605434059251No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACTGTACCC36481.0464235720678339No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAACT32770.9400027537462423No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAACTGT31750.9107441999219772No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA30970.8883700117034216No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAC30180.8657089749179614No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG29810.8550955779424927No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG28100.8060444730018129No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG27640.7928494389242032No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA25250.7242926314340133No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAACTG24670.7076554145535489No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC24120.6918787433737982No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT23590.6766757693278564No Hit
GGCATCTGTATATTTATTTTAAACACTGCTACCGTAACTTTAAAGCCATA21910.6284852100878904No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAACTGT20020.5742708309429286No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAC19440.5576336140624641No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAACTGTAGG18690.5361199715446222No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAACTGTA18160.5209169974986805No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA18070.5183353603965395No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG17860.5123115404915437No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA16740.480184500998233No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC16410.4707184982903826No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAACTGTA15690.45006540147325425No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAACTGTAGGACATC15530.44547582440278133No Hit
TGATACAGTAGCCAGAATGACGACACTGACGACATGGTTCTACATGAAAA15030.4311333960575533No Hit
TCATGCTGTTTACATTCACTAAGGCTACGGTAGCAGAGTCACAGTCACAG14440.4142093306101843No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT12790.3668793170709319No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG12360.3545448286940358No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA11560.33159694334167106No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT11380.326433669137389No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAACTGT10480.30061729811597865No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC9630.27623516992909103No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAACTGTA9580.2748009270945682No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAC9250.26533492438671774No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAG9070.2601716501824357No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC8420.2415264933336393No Hit
CTGCAGAGGTACATCCAATAAGTCTCTGCTACCGTAAGTGGAACCAAATG8390.24066594763292562No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG8320.23865800766459372No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG8310.23837115909768913No Hit
GGCATATTAGAGTTTCCTTTCTTGCCTCCAGCGGTGAAGCCTCATATGTT8310.23837115909768913No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT8250.2366500676962618No Hit
AGCTGCTCACCATCGCTACCGTATTGAGACTGTGGTGCCACCTAAGACCA7870.2257498221538885No Hit
ACGCCTTCTGTCTGAGCACACTGACGACATGGTTCTACTTCACCTTCTCT7710.22116024508341556No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTAACTGTAG6940.19907290543176448No Hit
TTGGACTCAATTTCAACTCTAACTTTAAATGCAAACTAGGCCAGGCAGAC6940.19907290543176448No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG6360.1824356885513No Hit
AGTCTTCTCCTAAACCATAGTCTGCTACCGTAGCTGATGATTTGTCCCTG5730.16436422883631271No Hit
TTTTAGGTGCTTTTGAATTGTGGAACAGTTTTCTTAACCATATCTAGGAG5150.14772701195584825No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT5100.14629276912132547No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA4910.14084264635013882No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT4500.1290818551070519No Hit
GGATACAGTAGCCAGAATGACGACACTGACGACATGGTTCTACATGAAAA4220.1210500952337242No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTAACTGTAGGACAT4190.12018954953301055No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT3970.11387888106111023No Hit
ACTCCAAAAAATACTAAGAGGCCCAAGTCTCTGCTACCGTAATTGTGCAA3940.11301833536039654No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAACTGT3730.1069945154554008No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA3660.10498657548706886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)