FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348616
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG239316.864573054593019No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA198995.707999632833834No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC189315.43033022007022No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG167584.807008284186612No Hit
TCTAGATATGGTTAAGAAAACTGTTCCACAATTCAAAAGCACCTAAAATG160974.617401381462698No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG118213.3908369093788004No Hit
GCCTCTAGAAATCATGACTAGGTTTGTACGGTAGCCAAGACCTCTTCTTT97742.803657892925167No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT85462.4514078527663674No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG85452.4511210041994627No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG78892.2629483443100717No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA70142.011955848268582No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT70012.008226816898823No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT58941.6906854533354752No Hit
TGCACGTACCACATTTAATTACCTTGCAGAGACTTGGTCTTCTCTTGGTA55601.594878031989352No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT53211.5263212244991624No Hit
CTTGGCCATAAGAAGCTCCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG52971.519436858893453No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA45081.2931133396057555No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT44881.287376368267664No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTTAGGGCACTGATGG40681.166899970167749No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGTAGTGAGGATGAAGAGCTTCCC40141.1514101475549028No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT40001.1473942676182391No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC38741.1112513481882644No Hit
CCATGGGACTGACTTTCTGCTACCGTAGACACCTACCTGATACCCCAGAT38261.0974826169768457No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT36541.0481446634692613No Hit
TTTGAAACAGAAGCAGAAGATGAAGTAGCAGAGACTTGGTCTTTTGAAAC34871.0002409527962No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA32680.9374211166441012No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC31750.9107441999219772No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA30750.8820593432315214No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG29970.8596851550129656No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC29180.8370241182275053No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT27770.7965784702939624No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT26380.7567065194942286No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT25050.7185556600959222No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT24700.7085159602542626No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG23900.6855680749018979No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT23480.6735204350919063No Hit
TGGCTTTGCATAATATGGCTTTAAAGTTACGGTAGCAGTGTTTAAAATAA20800.5966450191614843No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC19940.5719760424076921No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG19200.5507492484567547No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTCGTCA18890.5418569428827134No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG18430.5286619088051037No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT18270.5240723317346306No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG16740.480184500998233No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA16270.4667026183537187No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT15850.4546549785437272No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT15650.44891800720563596No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT15480.44404158156825846No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT14660.4205199990820846No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTAGCCTTAGTGAATGTAAAC13990.40130114509947906No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG12730.36515822566950457No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC12180.3493815544897538No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG11500.3298758519402437No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT11220.321844092066916No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC10330.2963145696124102No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG9560.27422722996075916No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT9540.27365353282695004No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG9210.26418753011909957No Hit
GGATCAGTATCATTTGGTTCCACTTACGGTAGCAGAGACTTATTGGATGT8400.2409527961998302No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG8300.23808431053078458No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT8290.23779746196388005No Hit
GCAGCAATTAACATATGAGGCTTCACCGCTGGAGGCAAGAAAGGAAACTC8260.23693691626316635No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC8170.2343552791610253No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC8000.22947885352364777No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTAGAACCATGTCGTCAGTGTGCTCA7800.2237418821855566No Hit
TAGGTGGCACCACAGTCTCAATACGGTAGCGATGGTGAGCAGCTTGTAGA7760.22259448791793834No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC6980.2002202996993827No Hit
GCCTGGCCTAGTTTGCATTTAAAGTTAGAGTTGAAATTGAGTCCAATGTA6880.1973518140303371No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT6120.17555132294559056No Hit
CAGACAGGGACAAATCATCAGCTACGGTAGCAGACTATGGTTTAGGAGAA5680.16292998600178993No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT5050.14485852628680268No Hit
CCTAGATATGGTTAAGAAAACTGTTCCACAATTCAAAAGCACCTAAAATG5040.1445716777198981No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC4940.14170319205085252No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4820.13826100924799778No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT4430.12707391513872No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG4370.12535282373729262No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4210.12076324666681965No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG3900.1118709410927783No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC3730.1069945154554008No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT3600.10326548408564151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)