FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479499
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA318966.651942965470209No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC266545.55871857918369No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC239324.9910427341871415No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA229484.785828541873914No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC225704.706996260680418No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT217394.533690372659796No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGAAAATGAAATACTTTGA206474.3059526714341425No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA171023.5666393464845596No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC166153.4650750053701884No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAATCAAGGTAATTAAATGT146683.0590261919211508No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA112012.3359798456305434No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA110712.3088682145322514No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA97762.0387946585915717No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA93801.9562084592460047No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC90621.889889238559413No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAAGG89101.858189485275256No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAAGGA78001.6266978658975306No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT57001.1887407481558876No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAAGGAGAA51301.069866673340299No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA50971.0629844900615018No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT49371.0296163287097575No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC49121.0244025534985475No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC48701.0156434111437147No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA41190.8590216037989651No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC38510.8031299335347936No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAAGG38100.7945793421884092No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG37110.7739327923520174No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG37010.7718472822675334No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG36320.7574572626845937No Hit
CTGTGGTGCCACCTAAGCTCTACGGTAGCAGCAAAAAGTCCTGCAACTTG35640.7432757941101025No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAAGGAG35510.7405646310002731No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG34490.7192924281385362No Hit
GACCCAAAACCCAAAATGGCTAAATCCATTTATTCCAAAAGAAAAAGCAA33120.6907209399811053No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA31450.6558929215702223No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT31190.6504705953505638No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC30790.6421285550126278No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAG29110.6070919855932964No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAAGGA27500.5735152732331037No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAAGGAGA27240.5680929470134453No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAAGGAGA27080.5647561308782708No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAACTGTCAAATGACCAAG24210.5049019914535797No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAG24020.50093952229306No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC23830.49697705313254037No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC21660.4517214842992373No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAAGGAG20890.4356630566487104No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAAGGAGAAG20150.42023028202352875No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT17540.3657984688184959No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG16820.3507827962102111No Hit
CACAAACCGTGCAAATTCAATACGGTAGCAGAAGCGCTGATCAGAAAGAC12740.26569398476326334No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT12630.2633999236703309No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAAGGAG12300.25651774039153363No Hit
GGAAAACCAGCGGAACCTCCTTGTGTTGTGTGTGCTGAGGTATGTCAAAG12250.25547498534929164No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAAGGAGA12010.25046976114653No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG11850.24713294501135563No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA11230.23420278248755472No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA11180.2331600274453127No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAAGGAGAAGGAATC11170.2329514764368643No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAG10500.21897855887082143No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG10370.2162673957609922No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG10160.21188782458357577No Hit
GTCCTCCAGAGCACAAACCTCCATTGGTTCAGCCATACCAGTCAAATGTC9950.20750825340615933No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT9460.19728925399218766No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAAGTGGGGTTTAGGGGCT9420.19645504995839408No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTAAGGAGAA8080.1685092148263083No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC7900.16475529667423708No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAAGTTTGTCCTCTTAATG7220.15057382809974576No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT6930.14452584885474212No Hit
GTACAATTTCTACCGCTGGTTCCCAGACCAAGTCTCTGCTACCGTAAGGA6610.13785221658439328No Hit
AAGAAGAACAAACAACAAGACCAAGTCTCTGCTACCGTAAGGAGAAGGAA5380.11220044254523992No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT5260.10969783044385911No Hit
TCGAATCAAGCACTAATACCAAGAGGGTTCTACATCCAATCAAGCAGTAG5200.10844652439316871No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTAAGGAGAAGGAAT5100.10636101430868468No Hit
ATGAGCAAGTTGGGGTGTGCATGGTGTGGGGGTTGTTGACTTGTAGACCA5080.10594391229178789No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT5020.10469260624109747No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTAAGGAGAAGGAAT4950.10323274918195868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)