FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479499
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT313456.53703135981514No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG264495.515965622451768No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG229374.7835344807809825No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG226644.726600055474568No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA223934.670082732185051No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC214934.48238682458149No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTAAGAACACAGAGGAGAATTTA205044.276129877226021No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT170863.5633025303493855No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA163773.4154398653594686No Hit
TGCACGTACCACATTTAATTACCTTGATTGGTCTTCTCTTGGTATTAGTG139142.9017787315510564No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT110872.3122050306674256No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT109692.287596011670514No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG96622.015019843628454No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT92931.938064521510994No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG89691.8704939947737118No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA89111.8583980362837045No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT77691.62023278463563No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC56611.1806072588264No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC50901.0615246330023629No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT50641.0561023067827044No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT49001.0218999413971666No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG48791.0175203702197502No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG48501.0114723909747465No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA41050.8561018896806876No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG38200.7966648522728931No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA38120.7949964442053059No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC36540.7620453848704585No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT36530.7618368338620101No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC35500.7403560799918248No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT32990.6880097768712761No Hit
ACAAGTTGCAGGACTTTTTGCTGCTACCGTAGAGCTTAGGTGGCACCACA32860.6852986137614469No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT31240.6515133503928059No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT31110.6488021872829766No Hit
CGTATACCACCACTTTTTCCCATGTTTATTTTGCTTTTTCTTTTGGAATA30420.6344121677000369No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG29640.6181451890410616No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA29410.6133485158467484No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG28950.6037551694581219No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT27300.5693442530641357No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT27280.5689271510472389No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT26910.5612107637346481No Hit
TGGTCATTTGACAGTTCTGGGTTACTTCTTTTAAACACCATCTTAAAATG24000.5005224202761632No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA23790.4961428490987468No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG23790.4961428490987468No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT21160.44129393387681726No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC20380.42502695521784195No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG19900.41501650681231866No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG17550.3660070198269444No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC16620.34661177604124305No Hit
TTCTGATCAGCGCTTCTGCTACCGTATTGAATTTGCACGGTTTGTGTGTA12570.2621486176196405No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT12560.26194006661119207No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC12470.2600631075351565No Hit
TTGACATACCTCAGCACACACAACACAAGGAGGTTCCGCTGGTTTTCCTG12060.251512516188772No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT11920.2485928020704944No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC11800.2460901899691136No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC11330.23628829257203873No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT11260.23482843551289992No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT10690.22294102803134103No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG10520.21939566088771822No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT10150.2116792735751274No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT10070.21001086550754017No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT9470.19749780500063607No Hit
ATGATCCATGGACATTTGACTGGTATGGCTGAACCAATGGAGGTTTGTGC9310.19416098886546165No Hit
AAAAGCCCCTAAACCCCACTTCTGGGTTACTTCTTTTAAACACCATCTTA9030.18832156062890643No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC8110.1691348678516535No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG7800.16266978658975306No Hit
TCACAGCCATTAAGAGGACAAACTTACGGTAGCAAATACAACAGCAAATC6910.14410874683784533No Hit
GGGAACCAGCGGTAGAAATTGTACTGTAGAACCATGTCGTCAGTGTAGAT6680.1393120736435321No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC6620.1380607675928417No Hit
TGTTGTTTGTTCTTCTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG5720.11929117683248558No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG5230.1090721774185139No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5180.10802942237627189No Hit
ACAAGTCAACAACCCCCACACCATGCACACCCCAACTTGCTCATTGTAGA5080.10594391229178789No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5020.10469260624109747No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT4990.10406695321575228No Hit
AGTTACAGCTACTGCTTGATTGGATGTAGAACCCTCTTGGTATTAGTGCT4860.10135579010592305No Hit
CGCACGTACCACATTTAATTACCTTGATTGGTCTTCTCTTGGTATTAGTG4800.10010448405523265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)