FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370149
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC364709.852789011992467No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC270417.305436459371767No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT246716.665153762403789No Hit
TCACAATTGCCAGTTAACGTCTCTGCGACATGGTTCTACAGGGAAGCTCT224536.065935609713926No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC185014.998257458482936No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA147283.9789382113689356No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA132863.5893653636778704No Hit
GCTGGTACTGAGCAAGAAGACATGGTGGTTGATCTTAAGGTAACATAGAC125663.3948491013078517No Hit
GGCTCCATAGGAAAGTAACAGCAACAGAGGAGGGGGAGACCAAGTCTCTG103432.7942801412404195No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA95552.5813928985354546No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT93442.5243888272020185No Hit
TGCAATTCTGAGGTGTTAAAGGGATACGGTAGCAGAAACTACCTGCTGCC90572.446852483729525No Hit
TTTCATATAGGCTGTGCAACAAGTCTCTGCTACCGTAACATCTGCAAGAA78772.128061942623106No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTC75672.044311885213792No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC52541.4194283923501076No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA49961.3497267316675177No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTATGGAGAGAAATCTGTATTAACA46291.250577470153911No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC41361.1173878627255511No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC40891.104690273376397No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACTCAAGA38851.0495773323715585No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACTCA31710.8566820388546235No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC30320.8191295937581894No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACTC29960.8094037806396883No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG26760.7229521084752356No Hit
AGCTGCTCACCATCGCTACCGTATCATTGCCTTACTGAAAGTCAGAAAGA26550.7172787174894435No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT24270.655681901072271No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACTCA24190.6535206092681596No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC22390.604891543675655No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACTCAA20930.5654479682506234No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACTCAA17420.4706212903452393No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC17420.4706212903452393No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTCAGTGTAAGCAAAGAAATAAT16880.456032570667488No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG16580.44792772640207046No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT15790.42658496983647126No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACTCAAG15750.42550432393441556No Hit
GTTGTATCCGCTGCTTTGTCCACACTGACACAATGGCAACAGTTCTGAGA14900.4025405985157328No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG14390.3887623632645232No Hit
AATGAAGAACTTGCTAGATAATCATACTGACACTGGCCATTCTTTACTGC14210.38389945670527276No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACTCAAGAA13700.3701212214540631No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACTCAAG12750.3444558812802412No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT12230.33040748455351765No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACTCAAGAAGCATC12040.3252744165187533No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG11940.3225728017636141No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC11710.31635908782679406No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC11440.3090647279879184No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCTCAATTTGGAAGCCTCTTAG11340.3063631132327792No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC9920.2680001837098034No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT9650.26070582387092767No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAGAGTCTAAAAAGTTACCCAG9530.2574638861647607No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA8860.23936306730532841No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA8850.2390929058298145No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACT8700.23504048369710576No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT8440.228016285333744No Hit
TTTGTGCTAAAACTTTGAGTCCCATCCAAAAGACCTATATCCAGGTAGAC8300.22423402467654918No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT8100.21883079516627088No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA8050.21747998778870128No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA8010.21639934188664567No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACTCAA5890.15912510907769575No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC5050.1364315451345269No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC4520.12211298693228943No Hit
TGGTGATTTGCTGATATACCATGTCTCTGCTACCGTAAGCAAATGTGTAT4450.12022185660369204No Hit
TGTCATAATGAATGCCCCATCGTACGGTAGCAGAACTGTCAAATGACCAA4300.11616943447098332No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTACT4270.11535895004444158No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG4220.114008142666872No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC3870.10455249102388497No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC3730.10077023036669018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)