FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370149
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG362419.790922034099783No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA266767.206827520809187No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC243926.589778710735407No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGCAGAGACGTTAACT211325.709052300560045No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA182364.926664667471748No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT144833.912748649868026No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT130723.5315508079178923No Hit
ATGTTACCTTAAGATCAACCACCATGTCTTCTTGCTCAGTACCAGCTGTA123323.3316313160375954No Hit
CCCCCTCCTCTGTTGCTGTTACTTTCCTATGGAGCCTGTAGAACCATGTC102842.7783406141850984No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT94972.565723532955648No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT91792.4798121837422227No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTATCCCTTTAACACCTCAGAATT89312.412812137814772No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA76222.0591707663670578No Hit
TGATGACTAGTTAATTTCTTGCAGATGTTACGGTAGCAGAGACTTGTTGC75452.038368332752486No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG52201.4102429021826346No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT49901.3481057628144342No Hit
CAGACTGTTAATACAGATTTCTCTCCATACGGTAGCAAAAGCGTCCAGAA45961.2416621414619518No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG39501.067137828279963No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC38721.0460652331898777No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG37841.0222910233446532No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT31950.8631659142669574No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG30070.8123755568703415No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA29970.8096739421152024No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT27090.7318674371671948No Hit
TTCTGACTTTCAGTAAGGCAATGATACGGTAGCGATGGTGAGCAGCTTGT26060.7040408051892616No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG24020.6489278641844229No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT24000.6483875412333951No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA22550.6092141272838776No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC20860.5635568379220259No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG17280.4668390296880445No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17280.4668390296880445No Hit
CCTGTCATGGGTATTATTTCTTTGCTTACACTGACGACCATAAATAACTT16420.4436051427938479No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT16220.43820191328356956No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT15650.4228027091792765No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT15550.42010109442413734No Hit
CAGAACTGTTGCCATTGTGTCAGTGTGGACAAAGCAGCGGATACAACTGT14710.3974075304809685No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT14320.3868712329359258No Hit
TGTGCAGTAAAGAATGGCCAGTGTCAGTATGATTATCTAGCAAGTTCTTC13780.3722825132581744No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG13610.3676897681744379No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT12800.3458066886578108No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT12410.3352703911127681No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC11780.3182502181553915No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG11670.3152784419247384No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA11380.3074437591348349No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT11220.3031211755266122No Hit
AAGAGGCTTCCAAATTGAGGGTTACTTCTTTTAAACACCATCTTAAAATG11210.3028510140510983No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG9910.26773002223428943No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC9500.25665340173821893No Hit
ACACTTCTGGGTAACTTTTTAGACTCTACGGTAGCAGAGACCTGTGGGAA9130.24665742714420408No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG8870.2396332287808423No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT8720.2355808066481336No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG8670.23422999927056404No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG8300.22423402467654918No Hit
ACCTGGATATAGGTCTTTTGGATGGGACTCAAAGTTTTAGCACAAATGTA8150.22018160254384045No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT8090.21856063369075696No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA8090.21856063369075696No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT7930.21423805008253433No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGCAGAGACGTTAACT6960.1880323869576846No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC5920.1599355935042375No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT4760.1285968623446234No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA4500.1215726639812616No Hit
GCTCTTTCTTGTAAATACACATTTGCTTACGGTAGCAGAGACATGGTATA4330.11697991889752506No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG4310.11643959594649722No Hit
TGGTCATTTGACAGTTCTGCTACCGTACGATGGGGCATTCATTATGACAT4280.11562911151995547No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT4260.11508878856892764No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA3940.10644362135248239No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA3780.10212103774425974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)