FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396640
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC350258.83042557482856No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC320438.078610326744656No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT249626.29336425978217No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA216655.46213190802743No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT165094.162212585720049No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA156773.952450584913272No Hit
GAGAAAGCCCCCCTACTGCTGCAGGCCGCCAGCGTAGTCTGATGTAGATG132613.3433340056474385No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA103902.619503832190399No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA101452.55773497377975No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCA84972.1422448567970953No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC74041.8666801129487696No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT70851.7862545381202097No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC65781.6584308188785801No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA61161.5419524001613554No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG60311.5205223880597014No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCAGATA58671.4791750705929811No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC53511.3490822912464704No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCAG43941.1078055667607907No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC38530.9714098426784994No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC36380.9172045179507865No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT35450.893757563533683No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCA35050.883672851956434No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGTACGGTAGCTGAACTTCGGA32590.8216518757563533No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC32550.8206434045986285No Hit
TCCTTGGCTCACAGTCTGGACACTGACGACATGGTTCTACATTCCTGCCA31250.7878680919725696No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACAAAGGT28540.7195441710367083No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC26730.6739108511496571No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA26210.6608007260992336No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCAGA25750.6492033077853974No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCAGAT25150.634076240419524No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTCAGTGTAAGCAAAGAAATAAT24720.6232351754739814No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC24610.620461879790238No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGCAG24010.6053348124243647No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG22900.577349737797499No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC22520.5677692617991125No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT22200.5597014925373134No Hit
GCCGGAACAGCCCCAGTTACCTGATTCCCGCGCCCAGAGACCAAGTCTCT20540.5178499394917305No Hit
ACATGTACATAGGGTTTATATATCATCAGCTACCGTATGAGGCCTTTGCA20050.5054961678096006No Hit
TGCTTGGATAGTTGAAATGACGACATGGTGGTTGATCTTAAGGTAACATA19850.5004538120209762No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG18940.47751109318273494No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGCAGA17150.43238200887454625No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG16680.42053247277127875No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG15820.39885034288019366No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA14990.3779245663574022No Hit
GCCCTCTCAGAAACATCTGCTACCGTAGTACATGCAGAGACCAAATTGCA14720.3711173860427592No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGCAGATAAGTATC13570.3421238402581686No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT13510.3406111335215813No Hit
AGAAATGTATAACCAAGTCAGTAAGGCACACTGACGACAGGTTTCTTAGA12630.3184247680516337No Hit
TGGGTCCAGTCAGTGAACGCATGGTTCTACAAACAATCGACAGGGGCCAG12580.3171641791044776No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGCAGATAA12350.3113654699475595No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT12240.3085921742638161No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGC12180.3070794675272287No Hit
GGATCCTGGGTGTTTGTATTTGCTACCGTATGGGAGGAGGGCAGTACACA11910.30027228721258575No Hit
AGTTGCTTAGCCTTTGCAATACCTACGGTAGCAGAAGCCTTTTTAGCCCT11670.2942214602662364No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT11190.28211980637353773No Hit
AGTCTTCAGAATGCCAGAGATACACTGACGACATGGTTCCCTCTGCGTGT10840.27329568374344493No Hit
TCCTCTCTTAGATTTGGCTTTCTGTACCACTGACTCTGATCCAGAAGACC10570.266488503428802No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA10220.2576643807987092No Hit
CTGATGTTGAATTAACCAAAAATATTCCCTCTGCGTGTTCTCATAAGACC9290.23421742638160548No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACAAAATGAAATA8090.2039632916498588No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTCGGTGTAAGCAAAGAAATAAT7270.1832896329164986No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGCAGA6730.16967527228721258No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC6680.1684146833400565No Hit
AAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCGTAGC6350.16009479628882614No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA6280.15832997176280758No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA5930.1495058491327148No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC5650.14244655102864057No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAGC5600.14118596208148448No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACAAAGGC5090.12832795482049214No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC5070.1278237192416297No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGCAGAT4970.12530254134731747No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC4500.11345300524405003No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG4460.11244453408632513No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT4370.11017547398144414No Hit
TAAAGGAACTGTAGTCGCCCTGCTGTGGTGGTGGTGGTAATTTTAAAGCC4370.11017547398144414No Hit
ACGATGGTTGTATTTGTCACCATACGGTAGCAGATACACACTGGTCCAGA4200.10588947156111336No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC4110.10362041145623235No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAGCA4070.10261194029850745No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG3990.10059499798305768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)