FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396640
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG346368.732351754739815No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA316227.97246873739411No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC245766.1960467930617185No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT213225.375655506252521No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC163404.119604679306172No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT154363.891690197660347No Hit
TCTCTTTAATTTCATCACTGTCCCTCATCTACATCAGACTACGCTGGCGG127003.2018959257765225No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA102832.5925272287212584No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT100312.5289935457845907No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA85132.146278741427995No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA72251.821551028640581No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT69641.755748285599032No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG64841.634731746672045No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT60691.530102864058088No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC58021.4627874142799515No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG53011.3364764017749091No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT43531.0974687373941105No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACAGGATTCCATT38430.9688886647841871No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG38220.9635941912061315No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG36470.9194735780556675No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA34970.8816559096409844No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT34740.8758572004840661No Hit
AGATCCACAGAACTTATAAAAACCTTTGTAGAACCATGTCGTCAGTGTAC32550.8206434045986285No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA32010.8070290439693425No Hit
GCAGAATTCCGAAGTTCAGCTACCGTACCTTTGGTTGCTAAAATAGAAAT31730.7999697458652681No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTCCAGAC30880.7785397337636143No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA26420.6660951996772893No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA25630.6461778943122226No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC25530.6436567164179104No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT24960.6292860024203307No Hit
CCTGTCATGGGTATTATTTCTTTGCTTACACTGACGACCATAAATAACTT24320.6131504638967326No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG24240.6111335215812828No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT23870.6018051633723275No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA22970.5791145623235175No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA22210.5599536103267446No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT22070.5564239612747076No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT20910.5271782977006857No Hit
CTGGGCGCGGGAATCAGGTAACTGGGGCTGTTCCGGCTGTAGAACCATGT20340.5128075837031061No Hit
AGGGATGCAAAGGCCTCATACGGTAGCTGATGATATATAAACCCTATGTA19620.49465510286405806No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCATTTCAACTATCCAAGCAT19480.491125453812021No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT18300.46137555465913677No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC16930.4268354175070593No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT16340.4119604679306172No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC15530.39153892698668813No Hit
TGCAATTTGGTCTCTGCATGTACTACGGTAGCAGATGTTTCTGAGAGGGC14660.36960467930617186No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA14660.36960467930617186No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT14270.35977208551835416No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT13350.33657724889068175No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGTGTGCCTTACTGACTTGGTT12560.31665994352561516No Hit
CCTCCTGGCCCCTGTCGATTGTTTGTAGAACCATGCGTTCACTGACTGGA12420.31313029447357804No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG12310.3103569987898346No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT12120.3055667607906414No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG12070.3043061718434853No Hit
GTGTACTGCCCTCCTCCCATACGGTAGCAAATACAAACACCCAGGATCCT11790.29724687373941105No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTAGGTATTGCAAAGGCTAAGCAACT11690.29472569584509883No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG11120.28035498184751917No Hit
TATGAGAACACGCAGAGGGAACCATGTCGTCAGTGTATCTCTGGCATTCT10500.26472367890278337No Hit
TCTGGATCAGAGTCAGTGGTACAGAAAGCCAAATCTAAGAGAGGATGTAG10370.2614461476401775No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT10000.25211778943122226No Hit
TATGAGAACACGCAGAGGGAATATTTTTGGTTAATTCAACATCAGTGTAG9340.23547801532876161No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTCTGTCTAAGAACACAGAGGAG8060.20320693828156516No Hit
CCTGTCATGGGTATTATTTCTTTGCTTACACCGACGACCATAAATAACTT7120.17950786607503028No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC6660.16791044776119401No Hit
AGATCCACAGAACTTATAAAAACCTTTGTAGAACCATGTCGTCAGTGTAG6390.161103267446551No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC6240.15732150060508268No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT6130.15454820492133925No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA5580.14068172650262203No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG5520.13916901976603469No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC5360.13513513513513514No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG5040.127067365873336No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT4810.1212686567164179No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA4450.11219241629689392No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT4440.11194029850746269No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC4210.10614158935054456No Hit
AGGGCTTTAAAATTACCACCACCACCACAGCAGGGCGACTACAGTTCCTT4150.10462888261395724No Hit
CTGGACCAGTGTGTATCTGCTACCGTATGGTGACAAATACAACCATCGTT4080.10286405808793868No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA4060.10235982250907623No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA3990.10059499798305768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)