FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649539
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC357545.504519359114695No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCACACTGACTTATGGTATCAAATA334155.1444178101699825No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC302654.659458477474024No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT280724.32183440871141No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT254403.916623944058786No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG237173.6513588868412827No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA203423.131759601809899No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC156262.405706200859379No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT153542.363830347369442No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT145852.245438688054143No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC140442.1621488471054087No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC135392.0844013985303422No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA111171.71152155605745No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA110301.698127441154419No Hit
CTCCCAAAATTCAAAATCCTCACACTGACGACATGTTTCTTCTATAACCC106531.6400862765746167No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT100431.5461735169096853No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC93961.4465644095273724No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTC90331.3906786197595526No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTCGATA76111.171754120999663No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA73121.1257214732294751No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT64550.9937817436674318No Hit
TCCTCCCCTCTTAGCTGTCTTAGTGAAAGGTATGATGAAGCTAGACCAAG64360.9908565921368847No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC61040.9397434180241679No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG59040.9089523492815674No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACACTGACGACATGGTTCTA57890.8912474847545722No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT55970.8616880587616756No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG54780.8433673728598283No Hit
AGAAAACGGCATTTTGAGTGTAATGGAAGCACAGCAAGTGGAAAATCTGT53560.824584820926842No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT51840.7981045018082056No Hit
CCCCTATTGCATATTTCTTCATGTGTACGGTAAGCAGCATCTTGAATCTC51270.7893290472165644No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTCG51240.7888671811854254No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC50080.7710083613147171No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG45990.708040625736099No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA45280.6971097963324758No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA44950.6920292699899467No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTC39360.6059682328543783No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA38480.5924201626076341No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC37820.5822591099225759No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT37660.5797958244231678No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTCG36850.5673254415824146No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT34580.532377578559563No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT33650.5180597315942538No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTCGA31790.48942403766363524No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTCGAT31320.4821881365091242No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC30900.4757220120731781No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT30660.47202708382406594No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC30320.46679260213782386No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA28690.4416978811126045No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG26030.40074575968494575No Hit
GGTGGTGTACCTGGTGAAAGACTAATGAATTCATCGGACATGTTACTGTT25930.3992062062478157No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA25790.39705083143583375No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT25410.39120052837473956No Hit
GGTAGGATGTGATATTCCTTCTAGTGGTACGGTAGCAGAAAGTCAGTCCC25380.39073866234360066No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA24650.3794999222525514No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTCGAT23760.36579789666209417No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA23680.36456625391239017No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC22750.3502484069470809No Hit
GGGATAATGGGGTTTTCCTGTGTACACTGACGACATTTGCTGCCATACAG21950.3379319794500407No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG21550.3317737657015206No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTCGA20680.3183796507984894No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC20180.31068188361283927No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG19990.3077567320822922No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG19470.29975105420921605No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG19410.2988273221469381No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATTCGATAG18880.29066768893014894No Hit
AGGACCCCATATAGCACAGGTACACTGACGACATGGTTCATCTGGACCTG18010.2772735740271177No Hit
TTGTTTTTATTGTGTGATACATGTTTACTTTTTAGACCAAGTACTGGAAA17730.2729628244031536No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTCGATAGCAATC17290.2661887892797815No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAACGTTCGAATGAGG16780.25833706675041834No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATT16610.2557198259072973No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA16390.2523328083456113No Hit
TTCAGAAGACAATGTTGATGTTCATGATACACTGACGACATGGTTCTACA16220.24971556750249024No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT15650.24094011291084907No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC15400.23709122931802404No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG15280.235243765193468No Hit
TCTCCACTTCACCCCGTCAGTGTAACCAGTTCACCTTAACCATTGCAGAC14680.2260064445706878No Hit
CAGTAGAGGCTAAGGATTCAGGTACACTGACGACATGGTGGTTGATCTTA14390.22154173960301074No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT14140.21769285601018568No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA13540.20845553538740552No Hit
CCAACTTTGTCCTTAACTAGCTCTGCTACCGTAGGGTGATGTGAGGAGTA12790.1969088846089303No Hit
ACACAGATTCTGAAATGAGAGCTATGCTGGAAATGTTTTAGATTCTGGTA11970.18428454642446412No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC11240.17304580633341493No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA10780.16596386052261683No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTATTCGA10310.1587279593681057No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT8710.13409510437402528No Hit
TTTGCAGACCTTCTCAGGAGTACACTGACGACATGGTTCTACATGCTAAT8530.13132390818719122No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA8490.13070808681233922No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC8320.1280908459692182No Hit
TTTCTTACCCTGTGCAGAGCATTGGAAGGACAGTGTGAAAATGATCCAGA8000.12316427497040208No Hit
TCTTGGTTGTCAGTGCTTCAGTACGGTAGCAGAGCAGTCAGAGGACCAGG7890.12147076618955906No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTATT7830.12054703412728104No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTATTCGAT7560.11639023984702998No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG7520.11577441847217795No Hit
CCCAAGATTACTGGCATCTTCCTGGAAGACCAAGTCTCTGCTACCGTATT6930.1066910531931108No Hit
GCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAGA6780.10438172303741577No Hit
GAGAAAGCCCCCCTACTGCTACTCGCATGGTTCTACAGAAAAACACAAGG6690.10299612494399873No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG6500.10007097341345168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)