FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649539
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG351075.404910251732383No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGGACACTACAAAGG329215.068363870375759No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA298044.588485064022329No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT276354.2545559235088275No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC252443.8864486966910374No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT199633.0734105265426708No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT168362.591992166752112No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG155192.3892329790820876No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT151382.3305759931274332No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT143442.2083354502193093No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA137752.120734859646611No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG129281.9903346835216977No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA108651.6727248094417735No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT108371.6684140598178094No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGAGGATTTTGAA103781.597748557053541No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC99501.531855669944376No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA92671.4267041701883951No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA90021.3859060041044495No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC75041.1552808992223715No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT72461.1155604205444167No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG68391.052900595653225No Hit
GTTGATACAAAACAAGCCCACGAACTGGCTAAGAGTTACGGGATTCCATT66891.0298072940962744No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG64320.9902407707620328No Hit
AGCTTCATCATACCTTTCACTAAGACAGCTAAGAGGGGAGGATGTAGAAC63700.9806955394518266No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG59900.9221925088408855No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTTAAG55890.8604564160119716No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC55120.8486018545460704No Hit
ACAGATTTTCCACTTGCTGTGCTTCCATTACACTCAAAATGCCGTTTTCT52700.8113446613675237No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT50710.7807075479686362No Hit
GTATGAGATTCAAGATGCTGCTTACCGTACACATGAAGAAATATGCAATA50660.7799377712500711No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG50140.7719320933769951No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG49680.764850147566197No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA49390.7603854425985199No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC45760.7044996528306999No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT44390.6834077707420185No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA43060.6629317100281892No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA38850.5981165103250151No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG37410.5759469408303427No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA37380.5754850747992037No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT37130.5716361912063787No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT36560.5628607366147376No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT33030.5085145002840477No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA31320.4821881365091242No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC31300.48188022582169815No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT30890.47556805672946506No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA30360.4674084235126759No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA30350.4672544681689629No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT28260.4350778013329453No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC25730.39612709937355567No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT25280.3891991089064706No Hit
CCATGGGACTGACTTTCTGCTACCGTACCACTAGAAGGAATATCACATCC24940.3839646272202285No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT24910.3835027611890895No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA24700.38026969897111645No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG23870.36749140544293724No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT23720.3651820752872422No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG22610.3480930321350989No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGTACACAGGAAAACCCCATTA21470.3305421229518166No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT21360.32884861417097355No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC21330.32838674813983454No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC20700.3186875614859154No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA19930.3068330000200142No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG19470.29975105420921605No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT19140.294670527866687No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG18950.29174537633613995No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG18780.2891281354930189No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT17690.2723470030283016No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAGTGTACCTGTGCTATATGG17530.26988371752889356No Hit
AGCCAACTGTATTCCTTTTCCAGTACTTGGTCTAAAAAGTAAACATGTAT17300.2663427446234945No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG16270.25048534422105523No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT15980.24602063925337814No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTATCATGAA15690.24155593428570107No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT15390.23693727397431102No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG15250.23478189916232897No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG14810.22800786403895687No Hit
GCAATGGTTAAGGTGAACTGGTTACACTGACGGGGTGAAGTGGAGATGTA14440.22231151632157575No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG14020.21584539188562965No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG13790.21230441898023059No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT13300.20476060713829347No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT13220.20352896438858942No Hit
TATTAAAAGGTTACTCCTCACATCACCCTACGGTAGCAGAGCTAGTTAAG12500.19244417964125327No Hit
ACCAGAATCTAAAACATTTCCAGCATAGCTCTCATTTCAGAATCTGTGTT11900.1832068590184731No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA10860.16719550327232083No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC10380.1598056467740967No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC10240.15765027196211467No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA8470.1304001761249132No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA8090.12454987306381911No Hit
GGATCATTTTCACACTGTCCTTCCAATGCTCTGCACAGGGTAAGAAATGT7810.12023912343985504No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG7780.11977725740871603No Hit
CCTGGTCCTCTGACTGCTCTGCTACCGTACTGAAGCACTGACAACCAAGA7760.11946934672129002No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT7340.1130032222853439No Hit
TTCAATTACCAGTTTATTTATGAAATCCCTTGGAGATTTGTAGAACCATG7280.11207949022306589No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT7280.11207949022306589No Hit
TCCAGGAAGATGCCAGTAATCTTGGGTGTAGAACCATGTCGTCAGTGTAG6780.10438172303741577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)