FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772790
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC324714.201788325418289No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC276843.5823444920353524No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG232203.004697265751368No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT227722.9467255011063807No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC215672.790796982362608No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA195122.524877392305801No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC179132.3179647769769276No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA169942.1990450186984822No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT147661.9107390105979634No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG140331.815887886748017No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT138431.7913016472780443No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT138071.7866432019047866No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA128761.6661706285019215No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG120941.5649788428939297No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC113561.469480712742142No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC106741.3812290531709779No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT103641.3411146624568124No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG100601.3017766793048564No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA99691.2900011646113434No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATCACTGGGTCAAAAACTA95371.234099820132248No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC86821.123461742517372No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTC84741.0965462803607706No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT83131.0757126774414782No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA79241.025375587158219No Hit
GGGATAATGGGGTTTTCCTGTGTAGACCATGCGGTGGGAGACAAAAACAG76040.9839671838403705No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG75840.9813791586330051No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC70880.9171961334903401No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT68960.8923510914996312No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC68300.883810608315325No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA68110.8813519843683278No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA65400.846284242808525No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA65230.8440844213822642No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC56920.736551974016227No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTCTAGC56330.7289172996544986No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT55590.7193416063872462No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG55550.7188240013457731No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC54000.6987668059886903No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG51710.6691339173643551No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT51530.6668046946777262No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTCT49120.6356189909289717No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC48750.6308311442953455No Hit
CTACAGTTATTTATTACCCCAGAAGCAAAGAAATAATACCCATGACAGGA44580.5768708187217744No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAATATATCATCTGGACCCTTT44550.5764826149406695No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG43830.5671657241941537No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA41980.5432264910260226No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC41620.5385680456527647No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA40890.5291217536458805No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT39950.5169580351712626No Hit
TTTTCTCCTTTCTGGAGATAATGCAAATGATCCCAAGTGGTCCAGACCAA39840.5155346213072115No Hit
GGATGCAGAGCTTCTTCCCACTTTGTGTAAGTTTGTCCTCTTAATGGCTG38230.49470101838791913No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTACGAATCAAGCACTAATACCAAG36330.4701147789179467No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT35870.46416232094100596No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT35160.45497483145485834No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC34970.4525162075078611No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT34190.4424229091991356No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA33150.4289651781208349No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA32260.4174484659480583No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA31550.4082609764619108No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG31430.40670816133749144No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTATTCTCCTCAGATGACTCCA29200.37785168027536586No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGTTGAGGTTTCAATGAATG28040.36284113407264584No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTC27600.35714747861644175No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG27310.3533948420657617No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC27280.3530066382846569No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTCTA27170.35158322442060586No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC26410.3417487286326169No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT26350.3409723210704072No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTCTAG26290.34019591350819756No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT26090.33760788830083205No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG26020.3367020794782541No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTCT24810.32104452697369273No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT23970.3101748211027575No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG23560.30486936942765824No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA22630.29283505221340855No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG22540.2916704408700941No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC22440.2903764282664113No Hit
CCAGACTCTCCAGAACCAAAGGACACTGACGACATGTTTCTTCTATAACC21780.2818359450821051No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC21530.27860091357289823No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG21510.27834211105216167No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCTGATATATGT21360.2764010921466375No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT21330.2760128883655327No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT20180.26113174342318096No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG19180.24819161738635337No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT18880.24430957957530505No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTCTAG17840.23085184849700435No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGTCTA17720.22929903337258503No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA16690.21597070355465262No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC16030.2074302203703464No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG15840.20497159642334917No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA15790.20432459012150778No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA15550.20121895987266916No Hit
CTGGAATATAATTTCAAACCACACATAGACCAAGTCTCTGCTACCGTAGT15340.19850153340493537No Hit
ATATATGTCTTCCACCCTTGACTCTCAGGATAGTGCAGCAGGATGCAGCG15080.1951371006353602No Hit
GAATTGTGCAGAGTGTGGTGTACACACTGACGACATGGTTCTACAGGGAA14770.19112566156394364No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGTCTAGCA14670.1898316489602609No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGT13930.18025595569300845No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG13610.1761151153612236No Hit
TCTGGGGTATCAGGTAGGTGTCGTCAGTGCAGCAACAGAGGAGGGGGAGA13120.1697744536031781No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT13000.1682216384787588No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT13000.1682216384787588No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT11900.15398749983824842No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTCTAGCAGGATC11680.15114067211014637No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC11580.1498466595064636No Hit
TGGTGATAGATGACTTGCTGCTACCGTACAAAGGTTGAATGGTGCTGAAG11100.14363539900878636No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG11060.14311779396731322No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA11030.14272959018620843No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT10530.1362595271677946No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA10410.1347067120433753No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC9910.1282366490249615No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC9910.1282366490249615No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA9640.12474281499501806No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT9430.12202538852728426No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA9340.12086077718396976No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA9330.1207313759236015No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA8480.10973226879229803No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC8310.10753244736603734No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGTCTA8100.10481502089830357No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC8060.10429741585683044No Hit
TTATTACTTAAAACCAGGAAACATGCCGTCAGTGTTGCTGGAAATGTTTT8040.10403861333609389No Hit
ATGGTTGATAGAGTGGTAGACCTTGGTCTGCTCCTAGCTAACATAATTGC7920.10248579821167458No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC7820.10119178560799183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)