FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772790
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG320334.145110573376985No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA272583.527219555118467No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT256193.315130889374863No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG239303.0965721606128445No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC224372.9033760788830083No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG213482.7624581063419558No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT190022.4588827495179806No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT167562.1682475187308325No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA142721.8468147879760348No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT136761.7696916367965425No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT135171.7491168363979865No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA125601.6252798302255462No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG121181.5680844731427686No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG114821.485785271548545No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA112041.449811721166164No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG106031.3720415636848302No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG102701.3289509439821945No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT98901.2797784650422495No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG96661.2507925827197557No Hit
TCATGATACTAGTTTTTGACCCAGTGATACGGTAGCAGATACATTGGACC91971.1901033916070343No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA83881.085417771969099No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT82821.0717012383700617No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA76130.9851317951836851No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC74880.9689566376376506No Hit
TTCTGGGGCTGTTTTTGTCTCCCACCGCATGGTCTACACAGGAAAACCCC72790.941911774220681No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG67220.8698352721955512No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA66570.8614241902716133No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT66500.8605183814490353No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA65340.8455078352463153No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT64600.8359321419790628No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT63760.8250624361081278No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCTCCGCGGAAAACGCCGC59330.7677376777649814No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG56440.7303407135185497No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC55530.7185651988250366No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA54980.7114481295047814No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG54810.7092483080785207No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC50500.6534763648597938No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA50370.6517941484750062No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG48980.6338073732838158No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT48610.6290195266501896No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG48330.6253962913598778No Hit
CCTGTCATGGGTATTATTTCTTTGCTTCTGGGGTAATAAATAACTGTAGT43960.5688479405789413No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC41740.540120860777184No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT39990.5174756402127356No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC39910.5164404301297895No Hit
GGACCACTTGGGATCATTTGCATTATCTCCAGAAAGGAGAAAATGTAGAA39580.5121701885376364No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG39410.5099703671113757No Hit
TCACAGCCATTAAGAGGACAAACTTACACAAAGTGGGAAGAAGCTCTGCA37460.48473712133956187No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG37450.4846077200791936No Hit
TCTCTTGGTATTAGTGCTTGATTCGTACGGTAGCAAAAGCGTCCAGAAAG35300.4567864491000142No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT35100.4541984238926487No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC34460.4459167432290791No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC33530.4338824260148294No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC31620.4091667852844887No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT31510.40774337142043765No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA31130.4028261235264431No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC30800.39855588193429003No Hit
TTAAATGGAGTCATCTGAGGAGAATACGGTAGCAGTTCTTTTGGTCATCA28410.36762898070627203No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC27690.3583120899597562No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA27440.3550770584505493No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC27140.35119502063950103No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA27070.3502892118169231No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT26760.3462777727455066No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT26040.33696088199899066No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA25550.3306202202409452No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC25370.3282909975543162No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA25170.32570297234695067No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT24670.3192329093285368No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT23600.3053869744691313No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA22810.2951642749000375No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA22170.2868825942364679No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG21540.2787303148332665No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT21290.27549528332405954No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA21100.27303665937706234No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC21000.2717426467733796No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT20950.27109564047153817No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTCCTTTGGTTCTG20920.27070743669043335No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT19810.25634389678955477No Hit
GGCATTCCTTTTTGAACAGTACCCGTTCCCTTGATGTCTACAATTTCACC18970.24547419091861955No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT18880.24430957957530505No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC18080.23395747874584297No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT17510.22658160690485124No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17500.22645220564448298No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC15620.2021247686952471No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA15470.20018374978972292No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT15350.19863093466530365No Hit
ATGTGTGGTTTGAAATTATATTCCAGTGTAGAACCATGTCGTCAGTGTAG15180.19643111323904297No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA15130.19578410693720155No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA15060.19487829811462362No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTGTACACC14660.1897022476998926No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG14560.18840823509620985No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA14000.18116176451558638No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG13720.17753852922527466No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC13510.17482110275754084No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG12920.16718642839581258No Hit
CCCCCTCCTCTGTTGCTGCACTGACGACACCTACCTGATACCCCAGATGT12900.16692762587507604No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT12830.16602181705249808No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA12720.16459840318844707No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTAGGGGGCTTTA12630.16343379184513257No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT12400.1604575628566622No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA11450.148164443121676No Hit
GGCATTCCTTTTTGAACAGTACCCGTTCCCTTGATGTCTACAATATCACC11160.144411806570996No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT10960.14182378136363047No Hit
TCAGCACCATTCAACCTTTGTACGGTAGCAGCAAGTCATCTATCACCATG10890.14091797254105254No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG10830.1401415649788429No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG10040.1299188654097491No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT9970.12901305658717116No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT9490.12280179608949392No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA9460.1224135923083891No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG9360.12111957970470633No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC9210.1191785607991822No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT8960.11594352928997527No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC8120.1050738234190401No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC8110.10494442215867182No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT8070.10442681711719873No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC7970.10313280451351597No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG7870.1018387919098332No Hit
GCAATTATGTTAGCTAGGAGCAGACCAAGGTCTACCACTCTATCAACCAT7850.10157998938909664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)